Transformation of Kluyveromyces fragilis.

Abstract:

:For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment by alkali cations and with the Kmr gene as the selective marker, a wild-type strain of K. fragilis was transformed to resistance against the antibiotic G418 . In the transformed cell the plasmid replicates autonomously. The same plasmid could also be used to transform S. cerevisiae trp1 mutant to Trp+. Thus, KARS2 of K. lactis enables the vector to replicate in K. fragilis, K. lactis, and S. cerevisiae, whereas ARS1 of S. cerevisiae allows autonomous replication only in S. cerevisiae.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Das S,Kellermann E,Hollenberg CP

doi

10.1128/JB.158.3.1165-1167.1984

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

1165-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

158

pub_type

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