Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction.

abstract：:The sequences of the capsular biosynthetic (cps) loci of 90 serotypes of Streptococcus pneumoniae have recently been determined. Bioinformatic procedures were used to predict the general functions of 1,973 of the 1,999 gene products and to identify proteins within the same homology group, Pfam family, and CAZy glycosy...

journal_title：Journal of bacteriology

authors： Aanensen DM,Mavroidi A,Bentley SD,Reeves PR,Spratt BG

更新日期：2007-11-01 00:00:00

abstract：:We have devised a coupled radiochemical assay for detecting phosphatidylglycerolphosphate (PGP) phosphatase activity in Escherichia coli colonies immobilized on filter paper. There appeared to be at least two enzymes capable of dephosphorylating PGP, as judged by the characterization of mutations in two genes designat...

journal_title：Journal of bacteriology

authors： Icho T,Raetz CR

更新日期：1983-02-01 00:00:00

abstract：:Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohyd...

journal_title：Journal of bacteriology

authors： Leighton TJ,Stock JJ,Kelln RA

更新日期：1970-08-01 00:00:00

abstract：:Sulfhydryl oxidants and stabilizers caused changes demonstrating the sulfhydryl content of beta-galactosidase for Streptococcus lactis 7962. Ammonium sulfate (0.85 m) rendered the enzyme insensitive to the oxidants. Titrations revealed 11.5 moles of sulfhydryl per mole of enzyme. ...

journal_title：Journal of bacteriology

authors： McFeters GA,Sandine WE,Elliker PR

更新日期：1971-10-01 00:00:00

abstract：:The gene (bdb) for protein thiol-disulfide oxidoreductase cloned from Bacillus brevis was found to encode a polypeptide consisting of 117 amino acid residues with a signal peptide of 27 residues. Bdb contains a well-conserved motif, Cys-X-X-Cys, which functions as the active center of disulfide oxidoreductases such as...

journal_title：Journal of bacteriology

authors： Ishihara T,Tomita H,Hasegawa Y,Tsukagoshi N,Yamagata H,Udaka S

更新日期：1995-02-01 00:00:00

abstract：:We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...

journal_title：Journal of bacteriology

authors： Park JT,Mahadevan S

更新日期：1988-08-01 00:00:00

abstract：:We identified several promoters responsible for the expression of regA, which encodes a global virulence regulator in Citrobacter rodentium. Expression of some of the promoters was strongly autoactivated by RegA in conjunction with bicarbonate. Biochemical and mutational analyses were used to determine the consensus s...

journal_title：Journal of bacteriology

authors： Tan A,Yang J,Tauschek M,Praszkier J,Robins-Browne RM

更新日期：2011-04-01 00:00:00

abstract：:The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phe...

journal_title：Journal of bacteriology

authors： Wehrl W,Niederweis M,Schumann W

更新日期：2000-07-01 00:00:00

abstract：:Salmonella typhimurium contains a transport system for sn-glycerol-3-phosphate that is inducible by growth on glycerol and sn-glycerol-3-phosphate. In fully induced cells, the system exhibited an apparent Km of 50 microM and a Vmax of 2.2 nmol/min . 10(8) cells. The corresponding system in Escherichia coli exhibits, u...

journal_title：Journal of bacteriology

authors： Hengge R,Larson TJ,Boos W

更新日期：1983-07-01 00:00:00

abstract：:The gene specifying a membrane-bound nucleoside diphosphate sugar hydrolase of Salmonella typhimurium was mapped near the metA locus by using intergeneric crosses between this bacterium and Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Minton N,Gunn J,Beacham IR

更新日期：1979-03-01 00:00:00

abstract：:West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...

journal_title：Journal of bacteriology

authors： Evans EE,Weinheimer PF,Painter B,Acton RT,Evans ML

更新日期：1969-06-01 00:00:00

abstract：:Rhodobacter sphaeroides maintained intracellular ammonium pools of 1.1 to 2.6 mM during growth in several fixed nitrogen sources as well as during diazotrophic growth. Addition of 0.15 mM NH4+ to washed, nitrogen-free cell suspensions was followed by linear uptake of NH4+ from the medium and transient formation of int...

journal_title：Journal of bacteriology

authors： Cordts ML,Gibson J

更新日期：1987-04-01 00:00:00

abstract：:The Escherichia coli K-12 hisT gene has been cloned, and its organization and expression have been analyzed on multicopy plasmids. The hisT gene, which encodes tRNA pseudouridine synthase I (PSUI), was isolated on a Clarke-Carbon plasmid known to contain the purF gene. The presence of the hisT gene on this plasmid was...

journal_title：Journal of bacteriology

authors： Marvel CC,Arps PJ,Rubin BC,Kammen HO,Penhoet EE,Winkler ME

更新日期：1985-01-01 00:00:00

abstract：:The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site, the two gene products TnpS and TnpT and (ii) the 2.4-kb tnpT-res-tnpS region is 48 kb apart from the tnpA gene (M. Tsuda,...

journal_title：Journal of bacteriology

authors： Genka H,Nagata Y,Tsuda M

更新日期：2002-09-01 00:00:00

abstract：:Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

journal_title：Journal of bacteriology

authors： Kosugi A,Murashima K,Doi RH

更新日期：2001-12-01 00:00:00

abstract：:We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli...

journal_title：Journal of bacteriology

authors： Daniel RA,Haiech J,Denizot F,Errington J

更新日期：1997-09-01 00:00:00

abstract：:The enterohepatic Helicobacter species Helicobacter hepaticus colonizes the murine intestinal and hepatobiliary tract and is associated with chronic intestinal inflammation, gall stone formation, hepatitis, and hepatocellular carcinoma. Thus far, the role of H. hepaticus motility and flagella in intestinal colonizatio...

journal_title：Journal of bacteriology

authors： Sterzenbach T,Bartonickova L,Behrens W,Brenneke B,Schulze J,Kops F,Chin EY,Katzowitsch E,Schauer DB,Fox JG,Suerbaum S,Josenhans C

更新日期：2008-10-01 00:00:00

abstract：:Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...

journal_title：Journal of bacteriology

authors： Krebs MP,Khorana HG

更新日期：1993-03-01 00:00:00

abstract：:Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with (14)C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 mumole per hr per 10(8) cells and was mainly via the Embden-Meyerhof path...

journal_title：Journal of bacteriology

authors： Stjernholm RL

更新日期：1967-05-01 00:00:00

abstract：:PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

journal_title：Journal of bacteriology

authors： Trott AE,Stevens AM

更新日期：2001-01-01 00:00:00

abstract：:Although BBA74 initially was described as a 28-kDa virulence-associated outer-membrane-spanning protein with porin-like function, subsequent studies revealed that it is periplasmic and downregulated in mammalian host-adapted spirochetes. To further elucidate the role of this protein in the Borrelia burgdorferi tick-ma...

journal_title：Journal of bacteriology

authors： Mulay VB,Caimano MJ,Iyer R,Dunham-Ems S,Liveris D,Petzke MM,Schwartz I,Radolf JD

更新日期：2009-04-01 00:00:00

abstract：:The function of the long propeptides of fungal proteinases is not known. Aspergillus fumigatus produces a 33-kDa serine proteinase of the subtilisin family and a 42-kDa metalloproteinase of the thermolysin family. These extracellular enzymes are synthesized as preproenzymes containing large amino-terminal propeptides....

journal_title：Journal of bacteriology

authors： Markaryan A,Lee JD,Sirakova TD,Kolattukudy PE

更新日期：1996-04-01 00:00:00

abstract：:The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of beta-mercaptoethanol or incubated with thiol-specific reagents. The enzyme was protected from inactivation in the presence ...

journal_title：Journal of bacteriology

authors： Pompeo F,van Heijenoort J,Mengin-Lecreulx D

更新日期：1998-09-01 00:00:00

abstract：:ICE6013 represents one of two families of integrative conjugative elements (ICEs) identified in the pan-genome of the human and animal pathogen Staphylococcus aureus Here we investigated the excision and conjugation functions of ICE6013 and further characterized the diversity of this element. ICE6013 excision was not ...

journal_title：Journal of bacteriology

authors： Sansevere EA,Luo X,Park JY,Yoon S,Seo KS,Robinson DA

更新日期：2017-03-28 00:00:00

abstract：:Sequences of the 16S ribosomal DNA (rDNA) from psychrotolerant and mesophilic strains of the Bacillus cereus group revealed signatures which were specific for these two thermal groups of bacteria. Further analysis of the genomic DNA from a wide range of food and soil isolates showed that B. cereus group strains have b...

journal_title：Journal of bacteriology

authors： Prüss BM,Francis KP,von Stetten F,Scherer S

更新日期：1999-04-01 00:00:00

abstract：:The SecY39(Cs) (cold-sensitive) alteration of Arg357 results in a defect of translocation initiation. As a means to dissect the Sec translocation machinery, we isolated mutations that act as suppressors of the secY39 defect. A specific secE mutation, designated secE105, was thus isolated. This mutation proved to be id...

journal_title：Journal of bacteriology

authors： Mori H,Akiyama Y,Ito K

更新日期：2003-02-01 00:00:00

abstract：:Mutants deficient in the production of bacteriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus. Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were ...

journal_title：Journal of bacteriology

authors： Pierson BK,Keith LM,Leovy JG

更新日期：1984-07-01 00:00:00

abstract：:The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which is mediated by short-chain fatty acids. During this fatty acid-mediated expression of spv, RpoS is required for inducti...

journal_title：Journal of bacteriology

authors： El-Gedaily A,Paesold G,Chen CY,Guiney DG,Krause M

更新日期：1997-02-01 00:00:00

abstract：:A method of assay of immunogenic potency of the cholera gut toxin is described; it is based on the relation of dose of antigen to neutralizing antibody titer produced in the rabbit under defined conditions and allows quantification of immunogenicity as immunogenic units per milligram of protein. Evidence, based on imm...

journal_title：Journal of bacteriology

authors： Kaur J,Burrows W,Cercavski L

更新日期：1969-11-01 00:00:00

abstract：:No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths ...

journal_title：Journal of bacteriology

authors： Tseng YH,Choy KT,Hung CH,Lin NT,Liu JY,Lou CH,Yang BY,Wen FS,Weng SF,Wu JR

更新日期：1999-01-01 00:00:00