Abstract:
:The SecY39(Cs) (cold-sensitive) alteration of Arg357 results in a defect of translocation initiation. As a means to dissect the Sec translocation machinery, we isolated mutations that act as suppressors of the secY39 defect. A specific secE mutation, designated secE105, was thus isolated. This mutation proved to be identical with the prlG2 mutation and to suppress a number of cold-sensitive secY mutations. However, other prlG mutations did not effectively suppress the secY defects. Evidence indicates that the Ser105-to-Pro alteration in the C-terminal transmembrane segment of SecE weakens SecY-SecE association. In vitro analyses showed that the SecE(S105P) alteration preferentially stimulates the initial phase of translocation. It is suggested that the S105P alteration affects the SecYEG channel such that it is more prone to open and to accept the translocation initiation domain of a preprotein molecule.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Mori H,Akiyama Y,Ito Kdoi
10.1128/jb.185.3.948-956.2003keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
948-56issue
3eissn
0021-9193issn
1098-5530journal_volume
185pub_type
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更新日期:1970-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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更新日期:1986-03-01 00:00:00
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更新日期:1978-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.99.3.834-841.1969
更新日期:1969-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.490-497.1978
更新日期:1978-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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更新日期:2001-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5487-5492.1996
更新日期:1996-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2902-2910.1996
更新日期:1996-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1058-1062.1976
更新日期:1976-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1967-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.1.320-322.1979
更新日期:1979-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章,收录出版
doi:10.1128/jb.186.7.2061-2067.2004
更新日期:2004-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.440-444.1984
更新日期:1984-02-01 00:00:00