Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli.

abstract：:The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

journal_title：Journal of bacteriology

authors： Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

更新日期：1989-05-01 00:00:00

abstract：:The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo rep...

journal_title：Journal of bacteriology

authors： Wray LV Jr,Reznikoff WS

更新日期：1983-12-01 00:00:00

abstract：:The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

journal_title：Journal of bacteriology

authors： Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

更新日期：2014-02-01 00:00:00

abstract：:Lactobacillus casei and Streptococcus faecalis accumulated labeled folic acid and metabolized this compound to poly-gamma-glutamates of chain lengths of up to 11 and 5, respectively. Octa- and nonaglutamates predominated in L. casei, and tetraglutamates predominated in S. faecalis. The most effective monoglutamate sub...

journal_title：Journal of bacteriology

authors： Shane B,Bognar AL,Goldfarb RD,LeBowitz JH

更新日期：1983-01-01 00:00:00

abstract：:Most motile bacteria are able to bias their movement towards more favorable environments or to escape from obnoxious substances by a process called chemotaxis. Chemotaxis depends on a chemosensory system that is able to sense specific environmental signals and generate a behavioral response. Typically, the signal is t...

journal_title：Journal of bacteriology

authors： Ringgaard S,Yang W,Alvarado A,Schirner K,Briegel A

更新日期：2018-03-12 00:00:00

abstract：:The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...

journal_title：Journal of bacteriology

authors： Chatfield LK,Orr E,Boulnois GJ,Wilkins BM

更新日期：1982-12-01 00:00:00

abstract：:Autoinducer-2 (AI-2) is required for biofilm formation and virulence of the oral pathogen Aggregatibacter actinomycetemcomitans, and we previously showed that lsrB codes for a receptor for AI-2. The lsrB gene is expressed as part of the lsrACDBFG operon, which is divergently transcribed from an adjacent lsrRK operon. ...

journal_title：Journal of bacteriology

authors： Torres-Escobar A,Juárez-Rodríguez MD,Lamont RJ,Demuth DR

更新日期：2013-01-01 00:00:00

abstract：:An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4...

journal_title：Journal of bacteriology

authors： Siegel EC

更新日期：1973-01-01 00:00:00

abstract：:PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...

journal_title：Journal of bacteriology

authors： Barendt SM,Land AD,Sham LT,Ng WL,Tsui HC,Arnold RJ,Winkler ME

更新日期：2009-05-01 00:00:00

abstract：:Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...

journal_title：Journal of bacteriology

authors： Sutcliffe J,Blumenthal R,Walter A,Foulds J

更新日期：1983-11-01 00:00:00

abstract：:West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...

journal_title：Journal of bacteriology

authors： Evans EE,Weinheimer PF,Painter B,Acton RT,Evans ML

更新日期：1969-06-01 00:00:00

abstract：:Rhodobacter sphaeroides maintained intracellular ammonium pools of 1.1 to 2.6 mM during growth in several fixed nitrogen sources as well as during diazotrophic growth. Addition of 0.15 mM NH4+ to washed, nitrogen-free cell suspensions was followed by linear uptake of NH4+ from the medium and transient formation of int...

journal_title：Journal of bacteriology

authors： Cordts ML,Gibson J

更新日期：1987-04-01 00:00:00

abstract：:Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...

journal_title：Journal of bacteriology

authors： Wookey PJ,Hussein S,Braun V

更新日期：1981-06-01 00:00:00

abstract：:Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...

journal_title：Journal of bacteriology

authors： Tokunaga T,Rashid MH,Kuroda A,Sekiguchi J

更新日期：1994-08-01 00:00:00

abstract：:Members of Thermococcales harbor a number of genes encoding putative aminotransferase Class III enzymes. Here, we characterized the TK1211 protein from the hyperthermophilic archaeon Thermococcus kodakarensis The TK1211 gene was expressed in T. kodakarensis under the control of the strong, constitutive promoter of the...

journal_title：Journal of bacteriology

authors： Zheng RC,Lu XF,Tomita H,Hachisuka SI,Zheng YG,Atomi H

更新日期：2021-01-19 00:00:00

abstract：:Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS). Earlier studies indicated that an alternate glucose transport system functions in this organism under conditions of high growth rates, low pH, or excess glucose. To identify this system, S. mutans...

journal_title：Journal of bacteriology

authors： Cvitkovitch DG,Boyd DA,Thevenot T,Hamilton IR

更新日期：1995-05-01 00:00:00

abstract：:Saccharomyces cerevisiae degrades allantoin in five steps to ammonia, CO(2), and glyoxylate. Previously we demonstrated that allophanic acid, the last intermediate of the pathway, was required for induction of all five degradative enzymes. The data presented here indicate that oxaluric acid, an allophanate analogue, i...

journal_title：Journal of bacteriology

authors： Sumrada R,Cooper TG

更新日期：1974-03-01 00:00:00

abstract：:Expression of virulence factors by Bordetella pertussis is altered by environmental signals (antigenic modulation) and is dependent on an activator encoded by a gene called vir. We have used TnphoA (Tn5 IS50L::phoA) gene fusions to define two sets of genes whose expression is either activated (vag loci) or repressed (...

journal_title：Journal of bacteriology

authors： Knapp S,Mekalanos JJ

更新日期：1988-11-01 00:00:00

abstract：:Chlamydia spp. exhibit a unique biphasic developmental cycle whereby infectious elementary bodies (EBs) invade host epithelial cells and differentiate into noninfectious, metabolically active reticulate bodies (RBs). EBs posses a unique outer envelope where rigidity is achieved by disulfide bonding among cysteine-rich...

journal_title：Journal of bacteriology

authors： Betts-Hampikian HJ,Fields KA

更新日期：2011-12-01 00:00:00

abstract：:Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...

journal_title：Journal of bacteriology

authors： Kwon M,Lee J,Lee C,Park C

更新日期：2012-04-01 00:00:00

abstract：:Mutations (env) which resulted in increased sensitivity of gonococci to diverse compounds were studied by transformation. Strains carrying an env mutation were more sensitive than wild-type strains to several antibiotics, dyes, and detergents. The env mutations resulted in complete phenotypic suppression of low-level ...

journal_title：Journal of bacteriology

authors： Sarubbi FA Jr,Sparling PF,Blackman E,Lewis E

更新日期：1975-11-01 00:00:00

abstract：:The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control elements, we show that the heat shock induction of 32 (out of 56) heat shock proteins is independent of RpoH and HrcA. These...

journal_title：Journal of bacteriology

authors： Rosen R,Büttner K,Becher D,Nakahigashi K,Yura T,Hecker M,Ron EZ

更新日期：2002-03-01 00:00:00

abstract：:We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footprinting with dimethyl s...

journal_title：Journal of bacteriology

authors： Ingmer H,Cohen SN

更新日期：1993-09-01 00:00:00

abstract：:DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

journal_title：Journal of bacteriology

authors： Asai T,Kogoma T

更新日期：1994-11-01 00:00:00

abstract：:Analysis of two virulence mutants of Pseudomonas syringae B728a revealed that the Tn 5 sites of insertion were within the gidA open reading frame (ORF). These mutations were pleiotropic, affecting diverse phenotypic traits, such as lipodepsipeptide (syringomycin and syringopeptin) antibiotic production, swarming, pres...

journal_title：Journal of bacteriology

authors： Kinscherf TG,Willis DK

更新日期：2002-04-01 00:00:00

abstract：:In Listeria monocytogenes, the full details of how stress signals are integrated into the σB regulatory pathway are not yet available. To help shed light on this question, we investigated a collection of transposon mutants that were predicted to have compromised activity of the alternative sigma factor B (σB). These m...

journal_title：Journal of bacteriology

authors： Guerreiro DN,Wu J,Dessaux C,Oliveira AH,Tiensuu T,Gudynaite D,Marinho CM,Boyd A,García-Del Portillo F,Johansson J,O'Byrne CP

更新日期：2020-04-09 00:00:00

abstract：:Between 59 and 60 min on the Escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. About 24 kilobase pairs downstream of the 14 repeats, a similar...

journal_title：Journal of bacteriology

authors： Nakata A,Amemura M,Makino K

更新日期：1989-06-01 00:00:00

abstract：:A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...

journal_title：Journal of bacteriology

authors： Hurlbert JC,Preston JF 3rd

更新日期：2001-03-01 00:00:00

abstract：:Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...

journal_title：Journal of bacteriology

authors： Skurray RA,Reeves P

更新日期：1974-01-01 00:00:00

abstract：:Two key elements that are thought to be required for replication initiation in eubacteria are the DnaA protein, a trans-acting factor, and the replication origin, a cis-acting element. As a first step in studying the replication initiation process in mycobacteria, we have isolated a 4-kb chromosomal DNA fragment from ...

journal_title：Journal of bacteriology

authors： Rajagopalan M,Qin MH,Nash DR,Madiraju MV

更新日期：1995-11-01 00:00:00