Abstract:
:The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo repressor titration assay. The results indicate that repressor specifically binds both regions of dyad symmetry.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Wray LV Jr,Reznikoff WSdoi
10.1128/JB.156.3.1188-1191.1983subject
Has Abstractpub_date
1983-12-01 00:00:00pages
1188-91issue
3eissn
0021-9193issn
1098-5530journal_volume
156pub_type
杂志文章abstract::We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts transla...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.757-763.1973
更新日期:1973-11-01 00:00:00
abstract::The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.527-535.1978
更新日期:1978-02-01 00:00:00
abstract::Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.2.782-784.1975
更新日期:1975-05-01 00:00:00
abstract::The origin of replication of the plasmid R1162 contains an initiation site for the synthesis of each DNA strand. When one of these sites (oriL) is deleted, synthesis on the corresponding strand is no longer initiated efficiently in vitro by the R1162-encoded replication proteins, and the plasmid is no longer stably ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.16.4870-4876.1996
更新日期:1996-08-01 00:00:00
abstract::The function of the long propeptides of fungal proteinases is not known. Aspergillus fumigatus produces a 33-kDa serine proteinase of the subtilisin family and a 42-kDa metalloproteinase of the thermolysin family. These extracellular enzymes are synthesized as preproenzymes containing large amino-terminal propeptides....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2211-2215.1996
更新日期:1996-04-01 00:00:00
abstract::We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A. 73:64-68, 1976), have been used to refine the map positions of tra cistrons on the F factor as well as to define a new D...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1383-1392.1978
更新日期:1978-03-01 00:00:00
abstract::The regulatory system that controls the expression of the Ti plasmid-borne octopine degradation (uad) and transfer (tra) genes in Agrobacterium tumefaciens was studied. A deletion mutant derived from the cointegrate plasmid R702::Ti-B6S3 was isolated, which was compatible with a wild-type Ti plasmid and which had reta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.424-431.1979
更新日期:1979-08-01 00:00:00
abstract::Expression of a circadian rhythm of conidiation in Neurospora crassa is enhanced in cultures grown on medium that is neutral or deficient in biotin. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.387-389.1975
更新日期:1975-07-01 00:00:00
abstract::The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.6.1841-1843.1993
更新日期:1993-03-01 00:00:00
abstract::The adherence of Ruminococcus albus 8 to crystalline cellulose was studied, and an affinity-based assay was also used to identify candidate cellulose-binding protein(s). Bacterial adherence in cellulose-binding assays was significantly increased by the inclusion of either ruminal fluid or micromolar concentrations of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.22.5921-5927.1998
更新日期:1998-11-01 00:00:00
abstract::Using nitrogen-fixing Sinorhizobium species that interact with Medicago plants as a model system, we aimed at clarifying how sex has shaped the diversity of bacteria associated with the genus Medicago on the interspecific and intraspecific scales. To gain insights into the diversification of these symbionts, we inferr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00105-07
更新日期:2007-07-01 00:00:00
abstract::Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.7.1900-1902.1995
更新日期:1995-04-01 00:00:00
abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1180-1191.1964
更新日期:1964-05-01 00:00:00
abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2247-2258.1997
更新日期:1997-04-01 00:00:00
abstract::The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.533-535.1983
更新日期:1983-04-01 00:00:00
abstract::An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruptio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.4.1003-1009.2002
更新日期:2002-02-01 00:00:00
abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.18.6198-6207.2004
更新日期:2004-09-01 00:00:00
abstract::Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.544-553.1968
更新日期:1968-08-01 00:00:00
abstract::The ribosome content was markedly reduced when illumination (at 28 C) was repeatedly interrupted; an opposite trend was observed with continuous light as the temperature was decreased. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1518-1519.1969
更新日期:1969-03-01 00:00:00
abstract::The anti-inflammatory drug phenylbutazone has been found to inhibit both engulfment and intracellular killing of E. coli by guinea pig peritoneal polymorphonuclear (PMN) leukocytes. The bactericidal activity of leukocytic homogenates was also inhibited by the drug. Addition of the drug at various time intervals to a p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.6.1982-1990.1968
更新日期:1968-12-01 00:00:00
abstract::A protein carboxyl methyltransferase (EC 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. This enzyme catalyzes the transfer of methyl groups from S-adenosylmethionine to the carboxyl groups of D-aspartyl o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.355-361.1992
更新日期:1992-01-01 00:00:00
abstract::A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to investigate the effect of induction and carbon catabolite derepression on the synthesis of multiple forms of endoglucana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3689-3693.1988
更新日期:1988-08-01 00:00:00
abstract::A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.471-474.1975
更新日期:1975-02-01 00:00:00
abstract::The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.30-35.1970
更新日期:1970-04-01 00:00:00
abstract::The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.973-976.1982
更新日期:1982-05-01 00:00:00
abstract::Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.2971-2976.1995
更新日期:1995-06-01 00:00:00
abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01569-07
更新日期:2008-10-01 00:00:00
abstract::Microbacterium yannicii is a Gram-positive, aerobic, yellow-pigmented, rod-shaped, nonmotile, oxidase-negative, and catalase-positive bacterium isolated on Columbia colistin-nalidixic acid (CNA) agar with 5% sheep blood from the sputum of a cystic fibrosis patient. The present study reports the draft genome of a Micro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01088-12
更新日期:2012-09-01 00:00:00
abstract::lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in devel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.11.3587-3590.1991
更新日期:1991-06-01 00:00:00
abstract::Mutations (env) which resulted in increased sensitivity of gonococci to diverse compounds were studied by transformation. Strains carrying an env mutation were more sensitive than wild-type strains to several antibiotics, dyes, and detergents. The env mutations resulted in complete phenotypic suppression of low-level ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.2.750-756.1975
更新日期:1975-11-01 00:00:00