Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10.

Abstract:

:The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo repressor titration assay. The results indicate that repressor specifically binds both regions of dyad symmetry.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wray LV Jr,Reznikoff WS

doi

10.1128/JB.156.3.1188-1191.1983

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

1188-91

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

156

pub_type

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