Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli.


:The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is formed as a precursor protein with a signal peptide of 21 amino acids; the mature form, with a molecular weight of 30,815, was previously found in the periplasm. The fecB genes of E. coli B and E. coli K-12 differed in 3 nucleotides, of which 2 gave rise to conservative amino acid exchanges. The fecC and fecD genes were found to encode very hydrophobic polypeptides with molecular weights of 35,367 and 34,148, respectively, both of which are localized in the cytoplasmic membrane. The fecE product was a rather hydrophilic but cytoplasmic membrane-bound protein of Mr 28,189 and contained regions of extensive homology to ATP-binding proteins. The number, structural characteristics, and locations of the FecBCDE proteins were typical for a periplasmic-binding-protein-dependent transport system. It is proposed that after FecA- and TonB-dependent transport of iron(III) dicitrate across the outer membrane, uptake through the cytoplasmic membrane follows the binding-protein-dependent transport mechanism. FecC and FecD exhibited homologies to each other, to the N- and C-terminal halves of FhuB of the iron(III) hydroxamate transport system, and to BtuC of the vitamin B12 transport system. FecB showed some homology to FhuD, suggesting that the latter may function in the same manner as a binding protein in iron(III) hydroxamate transport. The close homology between the proteins of the two iron transport systems and of the vitamin B12 transport system indicates a common evolution for all three systems.


J Bacteriol


Journal of bacteriology


Staudenmaier H,Van Hove B,Yaraghi Z,Braun V




Has Abstract


1989-05-01 00:00:00












  • Taxonomy of psychrophilic strains of Bacillus.

    abstract::The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Larkin JM,Stokes JL

    更新日期:1967-10-01 00:00:00

  • Identification of rhtX and fptX, novel genes encoding proteins that show homology and function in the utilization of the siderophores rhizobactin 1021 by Sinorhizobium meliloti and pyochelin by Pseudomonas aeruginosa, respectively.

    abstract::Rhizobactin 1021 is a hydroxymate siderophore produced by the soil bacterium Sinorhizobium meliloti 2011. A regulon comprising rhtA, encoding the outer membrane receptor protein for the ferrisiderophore; the biosynthesis operon rhbABCDEF; and rhrA, the Ara-C-like regulator of the receptor and biosynthesis genes has be...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cuív PO,Clarke P,Lynch D,O'Connell M

    更新日期:2004-05-01 00:00:00

  • The Bacillus subtilis tyrZ gene encodes a highly selective tyrosyl-tRNA synthetase and is regulated by a MarR regulator and T box riboswitch.

    abstract:UNLABELLED:Misincorporation of D-tyrosine (D-Tyr) into cellular proteins due to mischarging of tRNA(Tyr) with D-Tyr by tyrosyl-tRNA synthetase inhibits growth and biofilm formation of Bacillus subtilis. Furthermore, many B. subtilis strains lack a functional gene encoding D-aminoacyl-tRNA deacylase, which prevents misi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Williams-Wagner RN,Grundy FJ,Raina M,Ibba M,Henkin TM

    更新日期:2015-05-01 00:00:00

  • OmpC and LamB proteins can serve as substitute receptors for host range mutants of coliphage TuIa.

    abstract::Coliphage TuIa, which uses the OmpF protein as a receptor, can adapt by mutation to using instead the OmpC or LamB protein, or both. Most of the phage mutants retained the ability to use the OmpF protein, when present, but one class of mutants lost this ability and could only use the OmpC protein. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Moreno F,Wandersman C

    更新日期:1980-12-01 00:00:00

  • Characterization of the primary immunity region of the Escherichia coli linear plasmid prophage N15.

    abstract::N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This region contains a gene, cB, whose product shows homology to lambdoid ph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lobocka MB,Svarchevsky AN,Rybchin VN,Yarmolinsky MB

    更新日期:1996-05-01 00:00:00

  • Extracellular loops of lipid A 3-O-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonella enterica serovar typhimurium.

    abstract::Salmonella enterica serovar Typhimurium modifies its lipopolysaccharide (LPS), including the lipid A portion, in response to changes in its environment including host tissues. The lipid A 3-O-deacylase PagL, the expression of which is promoted under a host-mimetic environment, exhibits latency in S. enterica; deacylat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Manabe T,Kawasaki K

    更新日期:2008-08-01 00:00:00

  • A study of the double mutation of dnaJ and cbpA, whose gene products function as molecular chaperones in Escherichia coli.

    abstract::The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. To gain insight into the function of CbpA, we examined the nature of a cbpA null mutation with special reference to those of dnaK and dnaJ null mutations. In particular, the cbpA dnaJ double-null mutant was found to exhibit severe defec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ueguchi C,Shiozawa T,Kakeda M,Yamada H,Mizuno T

    更新日期:1995-07-01 00:00:00

  • Function and regulation of class I ribonucleotide reductase-encoding genes in mycobacteria.

    abstract::Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mowa MB,Warner DF,Kaplan G,Kana BD,Mizrahi V

    更新日期:2009-02-01 00:00:00

  • Induction of anaerobic gene expression in Rhodobacter capsulatus is not accompanied by a local change in chromosomal supercoiling as measured by a novel assay.

    abstract::In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cook DN,Armstrong GA,Hearst JE

    更新日期:1989-09-01 00:00:00

  • Control of cell growth in bacteria: experiments with thymine starvation.

    abstract::When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kubitschek HE

    更新日期:1971-02-01 00:00:00

  • Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.

    abstract::Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gregory MA,Till R,Smith MC

    更新日期:2003-09-01 00:00:00

  • Rhizobium japonicum nitrogenase Fe protein gene (nifH).

    abstract::A 12.1-kilobase PstI fragment from Rhizobium japonicum, which contains homology to both the Klebsiella pneumoniae and the Rhizobium meliloti nifH genes, was cloned into vector pHE3 . The nifH -homologous region was localized on the restriction enzyme cleavage map by Southern blot hybridization experiments. DNA fragmen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fuhrmann M,Hennecke H

    更新日期:1984-06-01 00:00:00

  • Introduction of the Escherichia coli gdhA gene into Rhizobium phaseoli: effect on nitrogen fixation.

    abstract::Rhizobium phaseoli lacks glutamate dehydrogenase (GDH) and assimilates ammonium by the glutamine synthetase-glutamate synthase pathway. A strain of R. phaseoli harboring the Escherichia coli GDH structural gene (gdhA) was constructed. GDH activity was expressed in R. phaseoli in the free-living state and in symbiosis....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bravo A,Becerril B,Mora J

    更新日期:1988-02-01 00:00:00

  • Identification of essential glutamates in the acetate kinase from Methanosarcina thermophila.

    abstract::Acetate kinase catalyzes the reversible phosphorylation of acetate (CH3COO- + ATP<-->CH3CO2PO3(2-) + ADP). A mechanism which involves a covalent phosphoryl-enzyme intermediate has been proposed, and chemical modification studies of the enzyme from Escherichia coli indicate an unspecified glutamate residue is phosphory...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Singh-Wissmann K,Ingram-Smith C,Miles RD,Ferry JG

    更新日期:1998-03-01 00:00:00

  • Partial purification and properties of D-glucosamine 6'-phosphate N-acetyltransferase from zoospores of Blastocladiella emersonii.

    abstract::A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Giddings TH,Cantino EC

    更新日期:1974-11-01 00:00:00

  • Role of the electrochemical proton gradient in genetic transformation of Haemophilus influenzae.

    abstract::The uptake of homologous DNA by Haemophilus influenzae was studied as a function of the proton motive force in completely competent cultures in the pH range of 6 to 8. The composition and magnitude of the proton motive force were varied by using the ionophores valinomycin and nigericin (in the presence of various pota...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bremer W,Kooistra J,Hellingwerf KJ,Konings WN

    更新日期:1984-03-01 00:00:00

  • Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus.

    abstract::Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kasai K,Nishizawa T,Takahashi K,Hosaka T,Aoki H,Ochi K

    更新日期:2006-10-01 00:00:00

  • Complete genome sequence of Carnobacterium sp. 17-4.

    abstract::Members of the carnobacteria have been extensively studied as probiotic cultures in aquacultures and protective cultures in seafood, diary, and meat. We report on the finished genome sequence of Carnobacterium sp. 17-4, which has been isolated from permanently cold seawater. The genetic information reveals a new circu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Voget S,Klippel B,Daniel R,Antranikian G

    更新日期:2011-07-01 00:00:00

  • Secretion of nucleoside diphosphate kinase by mucoid Pseudomonas aeruginosa 8821: involvement of a carboxy-terminal motif in secretion.

    abstract::Nucleoside diphosphate kinase (Ndk) is a ubiquitous enzyme which functions in balancing the nucleotide pool of the cell. We have recently reported that in addition to being intracellular in both mucoid and nonmucoid Pseudomonas aeruginosa, Ndk is also secreted into the extracellular environment by mucoid P. aeruginosa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kamath S,Chen ML,Chakrabarty AM

    更新日期:2000-07-01 00:00:00

  • Importance of the E-46-D-160 polypeptide segment of the non-penicillin-binding module for the folding of the low-affinity, multimodular class B penicillin-binding protein 5 of Enterococus hirae.

    abstract::Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mollerach ME,Partoune P,Coyette J,Ghuysen JM

    更新日期:1996-03-01 00:00:00

  • Characterization of the Campylobacter jejuni heptosyltransferase II gene, waaF, provides genetic evidence that extracellular polysaccharide is lipid A core independent.

    abstract::Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Oldfield NJ,Moran AP,Millar LA,Prendergast MM,Ketley JM

    更新日期:2002-04-01 00:00:00

  • Chromosome map of Xanthomonas campestris pv. campestris 17 with locations of genes involved in xanthan gum synthesis and yellow pigmentation.

    abstract::No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tseng YH,Choy KT,Hung CH,Lin NT,Liu JY,Lou CH,Yang BY,Wen FS,Weng SF,Wu JR

    更新日期:1999-01-01 00:00:00

  • Characterization of an ADP-ribosyltransferase toxin (AexT) from Aeromonas salmonicida subsp. salmonicida.

    abstract::An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Braun M,Stuber K,Schlatter Y,Wahli T,Kuhnert P,Frey J

    更新日期:2002-04-01 00:00:00

  • Mutations in motif II of Escherichia coli DNA helicase II render the enzyme nonfunctional in both mismatch repair and excision repair with differential effects on the unwinding reaction.

    abstract::Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brosh RM Jr,Matson SW

    更新日期:1995-10-01 00:00:00

  • Thermal adaptation in yeast: obligate psychrophiles are obligate aerobes, and obligate thermophiles are facultative anaerobes.

    abstract::The obligate psychrophilic yeasts Torulopsis psychrophila, T. austromarina, Leucosporidium frigidum, L. gelidum, and L. nivalis were obligate aerobes and were unable to grow anaerobically. In contrast, the obligate thermophilic yeasts T. bovina, T. pintolopesii, Candida slooffii, and Saccharomyces telluris were facult...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Watson K,Arthur H,Morton H

    更新日期:1978-11-01 00:00:00

  • Cloning, sequence analysis, and functional expression of the acetyl coenzyme A synthetase gene from Methanothrix soehngenii in Escherichia coli.

    abstract::In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the purified Acs. Afte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Eggen RI,Geerling AC,Boshoven AB,de Vos WM

    更新日期:1991-10-01 00:00:00

  • Genetic and molecular characteristics of Vir plasmids of bovine septicemic Escherichia coli.

    abstract::Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lopez-Alvarez J,Gyles CL,Shipley PL,Falkow S

    更新日期:1980-02-01 00:00:00

  • Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis.

    abstract::The kinA (spoIIJ) locus contains a single gene which codes for a protein of 69,170 daltons showing strong homology to the transmitter kinases of two component regulatory systems. The purified kinase autophosphorylates in the presence of ATP and mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Perego M,Cole SP,Burbulys D,Trach K,Hoch JA

    更新日期:1989-11-01 00:00:00

  • Genetic co-regulation of galactose and melibiose utilization in Saccharomyces.

    abstract::The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kew OM,Douglas HC

    更新日期:1976-01-01 00:00:00

  • Sulfolobus acidocaldarius synthesizes UMP via a standard de novo pathway: results of biochemical-genetic study.

    abstract::A genetic approach was used to establish the route of UMP biosynthesis in Sulfolobus acidocaldarius, a member of the hyperthermophilic division (the Crenarchaeota) of the Archaea domain. Pyrimidine auxotrophs of S. acidocaldarius DG6 were isolated by direct selection and by brute-force methods. Enzymatic assay of extr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Grogan DW,Gunsalus RP

    更新日期:1993-03-01 00:00:00