Abstract:
:Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose in the zones of inhibition on solid media, was no longer sensitive to lethal zygosis and exhibited the following properties: sensitivity to male-specific phage, donor ability, and surface exclusion. Since these characteristics were sensitive to acridine orange treatment, the strains carry an F factor extrachromosomally. They are, however, defective in some way since they retain sensitivity to female-specific phage. Temporary sensitivity to lethal zygosis in these and in standard F(+) strains can be induced by the formation of F(-) phenocopies. We have suggested that there is an immunity to lethal zygosis (Ilz(+)) associated with the F factor and discuss the results in terms of this hypothesis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Skurray RA,Reeves Pdoi
10.1128/JB.117.1.100-106.1974subject
Has Abstractpub_date
1974-01-01 00:00:00pages
100-6issue
1eissn
0021-9193issn
1098-5530journal_volume
117pub_type
杂志文章abstract::The DNA sequence of the gene which codes for the major outer membrane porin (Omp32) of Comamonas acidovorans has been determined. The structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. Comparisons with amino acid sequences of outer membrane protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.7.2196-2205.1991
更新日期:1991-04-01 00:00:00
abstract::In anaerobic cultures of Mucor rouxii, morphogenesis was strongly dependent on hexose concentration as well as pCO(2). At low levels of hexose or CO(2), or both, hyphal development occurred; at high levels, the fungus developed as yeast cells. Other dimorphic strains of Mucor responded similarly to hexose and CO(2) bu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1586-1594.1968
更新日期:1968-11-01 00:00:00
abstract::The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00213-10
更新日期:2010-06-01 00:00:00
abstract::The synthesis of pyruvate carboxylase (PC) was studied by using quantitative immunoblot analysis with an antibody raised against PC purified from Rhodobacter capsulatus and was found to vary 20-fold depending on the growth conditions. The PC content was high in cells grown on pyruvate or on carbon substrates metaboliz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.5.1460-1468.1997
更新日期:1997-03-01 00:00:00
abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01194-07
更新日期:2008-06-01 00:00:00
abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1287-1293.1962
更新日期:1962-06-01 00:00:00
abstract::Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.2.727-734.1979
更新日期:1979-02-01 00:00:00
abstract::Heterocyst differentiation is orchestrated by the N control transcriptional regulator NtcA and the differentiation-specific factor HetR. In Anabaena sp. strain PCC 7120, the devBCA operon is expressed from two different promoters activated upon N stepdown. The distal devB promoter (transcription start point [TSP] loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05994-11
更新日期:2012-06-01 00:00:00
abstract::Functional domains in the RepI replication initiator protein have been identified by classical and site-directed mutagenesis techniques. Mutations conferring an increase in plasmid copy number contained alterations in a key position of a putative helix-turn-helix DNA binding motif. The mutations did not appear to affe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.11.3563-3569.1993
更新日期:1993-06-01 00:00:00
abstract::Mild alkaline extraction of native poly-beta-hydroxybutyrate (PHB) granules results in the solubilization of a protein fraction. Both the solubilized protein fraction and the extracted granules are essentially devoid of PHB synthetase activity unless recombined. The protein fraction has been separated by chromatograph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.2.782-789.1971
更新日期:1971-11-01 00:00:00
abstract::Chlamydial lipopolysaccharide (LPS) agglutinated mouse and rabbit erythrocytes but not human, guinea pig, or pronghorn antelope erythrocytes. Hemagglutination was not specific for Chlamydia spp., as rough LPSs from Coxiella burnetii and Escherichia coli also agglutinated erythrocytes from the same animal species. Nona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3826-3828.1987
更新日期:1987-08-01 00:00:00
abstract::The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7210-7221.1995
更新日期:1995-12-01 00:00:00
abstract::Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.628-633.1966
更新日期:1966-02-01 00:00:00
abstract::The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo rep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.3.1188-1191.1983
更新日期:1983-12-01 00:00:00
abstract::Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.3.677-683.1997
更新日期:1997-02-01 00:00:00
abstract::The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2197-2204.1993
更新日期:1993-04-01 00:00:00
abstract::Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.2.595-603.1971
更新日期:1971-02-01 00:00:00
abstract::The formation of bacteriolytic enzymes of Staphylococcus aureus, with special reference to strain M18, was investigated under a variety of conditions. The bacteriolytic activity was tested by using whole cells of Micrococcus lysodeikticus as a substrate. Complex media were required for production, and a Casein Hydroly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.227-233.1970
更新日期:1970-10-01 00:00:00
abstract::The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3375-3380.1998
更新日期:1998-07-01 00:00:00
abstract::An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5904-5911.1994
更新日期:1994-10-01 00:00:00
abstract::During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3204-3210.2001
更新日期:2001-05-01 00:00:00
abstract::In Salmonella enterica serovar Typhimurium a mutation in the purF gene encoding the first enzyme in the purine pathway blocks, besides the synthesis of purine, the synthesis of thiamine when glucose is used as the carbon source. On carbon sources other than glucose, a purF mutant does not require thiamine, since the a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.750-759.2003
更新日期:2003-02-01 00:00:00
abstract::Deletions of the Escherichia coli K-12 chromosome between trpR and thr render the bacterium sensitive to the dye toluidine blue (Dye-), and if male (Hfr or F'), the strain is sterile (Fex-), failing to donate F' or chromosomal markers and resistant to male-specific phages as a consequence of its inability to elaborate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1309-1314.1983
更新日期:1983-06-01 00:00:00
abstract::In the chloroplasts of higher plants and algae, the biosynthesis of the chlorophyll precursor delta-aminolevulinic acid (ALA) involves at least three enzymes and a tRNA species. Here we demonstrate that in cell extracts of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 ALA was formed from glutamate i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3810-3816.1988
更新日期:1988-09-01 00:00:00
abstract::Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.641-648.1972
更新日期:1972-09-01 00:00:00
abstract::In Escherichia coli, T:G mismatches in specific contexts are corrected by a very short patch (VSP) repair system. Previous studies have shown that the product of gene vsr mediates correction of T:G to C:G in the 5'CTAGG/3'GGTCC context and in some related contexts. Amber mutations that arose in CAG sequences in gene c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.660-666.1995
更新日期:1995-02-01 00:00:00
abstract::Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be posttranslationally modified for full functionality; in enterobacteria, this is accomplished by two enzymes, namely, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00722-18
更新日期:2019-05-08 00:00:00
abstract::Mutants of Escherichia coli K-12 constitutive for fatty acid degradation (fadR) showed an increased rate of utilization of exogenous acetate. Acetate transport, oxidation, and incorporation into macromolecules was approximately fivefold greater in fadR mutants than fadR+ strains during growth on succinate as a carbon ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.1.83-90.1981
更新日期:1981-10-01 00:00:00
abstract::Two nifA-like genes, designated anfA and vnfA, have been identified in Azotobacter vinelandii. The anfA gene is located upstream from the nitrogenase-3 structural gene cluster (anfHDGK) and is preceded by a sequence that is potentially part of a ntrA-dependent promoter. The product of anfA appears to be required for e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3258-3267.1989
更新日期:1989-06-01 00:00:00
abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.821-824.2000
更新日期:2000-02-01 00:00:00