F factor-mediated immunity to lethal zygosis in Escherichia coli K-12.

Abstract:

:Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose in the zones of inhibition on solid media, was no longer sensitive to lethal zygosis and exhibited the following properties: sensitivity to male-specific phage, donor ability, and surface exclusion. Since these characteristics were sensitive to acridine orange treatment, the strains carry an F factor extrachromosomally. They are, however, defective in some way since they retain sensitivity to female-specific phage. Temporary sensitivity to lethal zygosis in these and in standard F(+) strains can be induced by the formation of F(-) phenocopies. We have suggested that there is an immunity to lethal zygosis (Ilz(+)) associated with the F factor and discuss the results in terms of this hypothesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Skurray RA,Reeves P

doi

10.1128/JB.117.1.100-106.1974

subject

Has Abstract

pub_date

1974-01-01 00:00:00

pages

100-6

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

117

pub_type

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