Genomic rearrangements leading to overexpression of aldo-keto reductase YafB of Escherichia coli confer resistance to glyoxal.

abstract：:Anabaena variabilis ATCC 29413 is a heterotrophic, nitrogen-fixing cyanobacterium that has been reported to fix nitrogen and reduce acetylene to ethane in the absence of molybdenum. DNA from this strain hybridized well at low stringency to the nitrogenase 2 (vnfDGK) genes of Azotobacter vinelandii. The hybridizing reg...

journal_title：Journal of bacteriology

authors： Thiel T

更新日期：1993-10-01 00:00:00

abstract：:Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified...

journal_title：Journal of bacteriology

authors： Zinser ER,Kolter R

更新日期：2000-08-01 00:00:00

abstract：:The pelA and pelC genes from Erwinia chrysanthemi EC16 were sequenced and overexpressed in Escherichia coli cells. These genes and two others from the same strain that were characterized previously encode catalytically related pectate lyase proteins that are involved with the maceration and soft-rotting of plant tissu...

journal_title：Journal of bacteriology

authors： Tamaki SJ,Gold S,Robeson M,Manulis S,Keen NT

更新日期：1988-08-01 00:00:00

abstract：:A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medi...

journal_title：Journal of bacteriology

authors： Daron HH

更新日期：1967-02-01 00:00:00

abstract：:Cultures of the inducible penicillinase-producing strain 749 of Bacillus licheniformis, induced with small amounts of benzylpenicillin, synthesized penicillinase at a high rate for a short period, after which the rate of synthesis slowly declined. During the period of active synthesis, the rate of secretion, as a frac...

journal_title：Journal of bacteriology

authors： Sargent MG,Ghosh BK,Lampen JO

更新日期：1969-02-01 00:00:00

abstract：:The divergence of Salmonella enterica and Escherichia coli is estimated to have occurred approximately 140 million years ago. Despite this evolutionary distance, the genomes of these two species still share extensive synteny and homology. However, there are significant differences between the two species in terms of g...

journal_title：Journal of bacteriology

authors： Bishop AL,Baker S,Jenks S,Fookes M,Gaora PO,Pickard D,Anjum M,Farrar J,Hien TT,Ivens A,Dougan G

更新日期：2005-04-01 00:00:00

abstract：:Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schnaitman CA

更新日期：1978-03-01 00:00:00

abstract：:Multitrichously polar flagellated mutants were isolated from a monotrichously flagellated strain of Pseudomonas aeruginosa. The ability of the mutant cells to swarm in semisolid media at given gel strengths was increased by the multiflagellation. Observations of the mutant cells by electron microscopy revealed that th...

journal_title：Journal of bacteriology

authors： Suzuki T,Iino T

更新日期：1980-09-01 00:00:00

abstract：:We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

journal_title：Journal of bacteriology

authors： Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

更新日期：2007-01-01 00:00:00

abstract：:During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

journal_title：Journal of bacteriology

authors： Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

更新日期：1990-02-01 00:00:00

abstract：:Shewanella putrefaciens strain 200 respires a wide range of compounds as terminal electron acceptor. The respiratory versatility of Shewanella is attributed in part to a set of c-type cytochromes with widely varying midpoint redox potentials (E'(0)). A point mutant of S. putrefaciens, originally designated Urr14 and h...

journal_title：Journal of bacteriology

authors： Dale JR,Wade R Jr,Dichristina TJ

更新日期：2007-02-01 00:00:00

abstract：:FtsZ and ZipA are essential components of the septal ring apparatus, which mediates cell division in Escherichia coli. FtsZ is a cytoplasmic tubulin-like GTPase that forms protofilament-like homopolymers in vitro. In the cell, the protein assembles into a ring structure at the prospective division site early in the di...

journal_title：Journal of bacteriology

authors： Hale CA,Rhee AC,de Boer PA

更新日期：2000-09-01 00:00:00

abstract：:In this report, we describe some of the characteristics of the Comamonas testosteroni B-356 biphenyl (BPH)-chlorobiphenyl dioxygenase system, which includes the terminal oxygenase, an iron-sulfur protein (ISPBPH) made up of an alpha subunit (51 kDa) and a beta subunit (22 kDa) encoded by bphA and bphE, respectively; a...

journal_title：Journal of bacteriology

authors： Hurtubise Y,Barriault D,Powlowski J,Sylvestre M

更新日期：1995-11-01 00:00:00

abstract：:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

journal_title：Journal of bacteriology

authors： Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

更新日期：2008-03-01 00:00:00

abstract：:Upon induction, Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills B. megaterium and a few other bacterial species. The DNA region responsible for megacinogeny was cloned in B. megaterium. The nucleotide sequence of a 5,494-bp-long subfragment was determ...

journal_title：Journal of bacteriology

authors： Kiss A,Balikó G,Csorba A,Chuluunbaatar T,Medzihradszky KF,Alföldi L

更新日期：2008-10-01 00:00:00

abstract：:The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...

journal_title：Journal of bacteriology

authors： McBeth DL

更新日期：1989-02-01 00:00:00

abstract：:The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genoty...

journal_title：Journal of bacteriology

authors： Brownell GH,Kelly KL

更新日期：1969-07-01 00:00:00

abstract：:Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the...

journal_title：Journal of bacteriology

authors： Sardesai AA,Gowrishankar J

更新日期：2001-06-01 00:00:00

abstract：:A pentachlorophenol (PCP) hydroxylase which catalyzed the conversion of PCP to 2,3,5,6-tetrachlorohydroquinone and released iodide from triiodophenol in the presence of NADPH and oxygen was identified. The enzyme was purified by protamine sulfate precipitation, ammonium sulfate precipitation, hydrophobic chromatograph...

journal_title：Journal of bacteriology

authors： Xun L,Orser CS

更新日期：1991-07-01 00:00:00

abstract：:A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...

journal_title：Journal of bacteriology

authors： Day JM,Janssen GR

更新日期：2004-10-01 00:00:00

abstract：:Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...

journal_title：Journal of bacteriology

authors： Clarke BR,Pearce R,Roberts IS

更新日期：1999-04-01 00:00:00

abstract：:The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...

journal_title：Journal of bacteriology

authors： Lohrke SM,Nechaev S,Yang H,Severinov K,Jin SJ

更新日期：1999-08-01 00:00:00

abstract：:A 200-megadalton plasmid was mobilized from Rhizobium japonicum USDA 191 to other Rhizobium strains either that cannot nodulate soybeans or that form Fix- nodules on certain cultivars. The symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nit...

journal_title：Journal of bacteriology

authors： Appelbaum ER,McLoughlin TJ,O'Connell M,Chartrain N

更新日期：1985-07-01 00:00:00

abstract：:Using transposon Tn5-mediated mutagenesis, an essential Rhizobium meliloti nitrogen fixation (nif) gene was identified and located directly downstream of the regulatory gene nifA. Maxicell and DNA sequence analysis demonstrated that the new gene is transcribed in the same direction as nifA and codes for a 54-kilodalto...

journal_title：Journal of bacteriology

authors： Buikema WJ,Klingensmith JA,Gibbons SL,Ausubel FM

更新日期：1987-03-01 00:00:00

abstract：:The utilization of glycerol as a carbon source for growth by Klebsiella aerogenes, strain 2103, involves separate aerobic (sn-glycerol-3-phosphate or G3P) and anaerobic (dihydroxyacetone or DHA) pathways of catabolism. Enzyme and transport activities of the aerobic pathway are elevated in cells grown under oxygenated ...

journal_title：Journal of bacteriology

authors： Ruch FE,Lengeler J,Lin EC

更新日期：1974-07-01 00:00:00

abstract：:While studying a set of multiply marked mutants of Staphylococcus aureus strain 8325 by transformation, several instances of apparent genetic linkage were encountered. After showing that these linked transformations were readily inactivated by shearing of the deoxyribonucleic acid (DNA) but were resistant to dilution ...

journal_title：Journal of bacteriology

authors： Pattee PA,Neveln DS

更新日期：1975-10-01 00:00:00

abstract：:Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

journal_title：Journal of bacteriology

authors： Shum AC,Markovetz AJ

更新日期：1974-06-01 00:00:00

abstract：:The steps in the biosynthetic transformation of GTP to 7,8-dihydro-D-erythro-neopterin (H2neopterin), the precursor to the modified folates found in the methanogenic archaea, has been elucidated for the first time in two members of the domain Archaea. In Methanococcus thermophila and Methanobacterium thermoautotrophic...

journal_title：Journal of bacteriology

authors： Howell DM,White RH

更新日期：1997-08-01 00:00:00

abstract：:It is thought that in the gram-negative soil bacterium Sinorhizobium meliloti the protein ExoP is involved in biosynthesis of the acidic exopolysaccharide succinoglycan (EPS I). The amounts and compositions of EPS I produced by mutants expressing ExoP proteins characterized by specific amino acid substitutions in the ...

journal_title：Journal of bacteriology

authors： Niemeyer D,Becker A

更新日期：2001-09-01 00:00:00

abstract：:The well-known large catabolic potential of rhodococci is greatly facilitated by an impressive gene multiplicity. This study reports on the multiplicity of kshA, encoding the oxygenase component of 3-ketosteroid 9α-hydroxylase, a key enzyme in steroid catabolism. Five kshA homologues (kshA1 to kshA5) were previously i...

journal_title：Journal of bacteriology

authors： Petrusma M,Hessels G,Dijkhuizen L,van der Geize R

更新日期：2011-08-01 00:00:00