Abstract:
:N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This region contains a gene, cB, whose product shows homology to lambdoid phage repressors. The cB3 mutation confers thermoinducibility on N15 lysogens, consistent with CB being the primary repressor of N15. Downstream of cB lies the locus of N15 plasmid replication. Upstream of cB lies an operon predicted to encode two products: one homologous to the late repressor of P22 (Cro), the other homologous to the late antiterminator of phi 82 (Q). The Q-like protein is essential for phage development. We show that CB protein regulates the expression of genes that flank the cB gene by binding to DNA at symmetric 16-bp sites. Three sites are clustered upstream of cB and overlap a predicted promoter of the cro and Q-like genes as well as two predicted promoters of cB itself. Two sites downstream of cB overlap a predicted promoter of a plasmid replication gene, repA, consistent with the higher copy number of the mutant, N15cB3. The leader region of repA contains terminators in both orientations and a putative promoter. The organization of these regulatory elements suggests that N15 plasmid replication is controlled not only by CB but also by an antisense RNA and by a balance between termination and antitermination.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lobocka MB,Svarchevsky AN,Rybchin VN,Yarmolinsky MBdoi
10.1128/jb.178.10.2902-2910.1996subject
Has Abstractpub_date
1996-05-01 00:00:00pages
2902-10issue
10eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to purified LPS and cells in ...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.197-203.1975
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.1-3.1977
更新日期:1977-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.1172-1179.1976
更新日期:1976-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5762-5766.1995
更新日期:1995-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2011-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4591-4598.1997
更新日期:1997-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4581-4588.1987
更新日期:1987-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3638-3646.1987
更新日期:1987-08-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1980-11-01 00:00:00
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更新日期:2009-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.118.2.725-734.1974
更新日期:1974-05-01 00:00:00
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doi:10.1128/jb.176.21.6672-6676.1994
更新日期:1994-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2020-03-26 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1970-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1975-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2000-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2009-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-06-01 00:00:00
abstract::Arginine utilization in Pseudomonas aeruginosa with multiple catabolic pathways represents one of the best examples of the metabolic versatility of this organism. To identify genes involved in arginine catabolism, we have employed DNA microarrays to analyze the transcriptional profiles of this organism in response to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00