Abstract:
:Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequenced. An analysis of these sequences and of the previously determined sequence from E. coli indicated that the vast majority of changes were synonymous rather than nonsynonymous changes. Nucleotide changes occurred at 89 of 492 positions but resulted in amino acid changes at only 2 of 164 positions. This analysis suggests that the Lrp amino acid sequence is highly adapted for function and that almost all amino acid changes lead to a protein that functions less well than the wild-type protein.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Friedberg D,Platko JV,Tyler B,Calvo JMdoi
10.1128/jb.177.6.1624-1626.1995subject
Has Abstractpub_date
1995-03-01 00:00:00pages
1624-6issue
6eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00959-09
更新日期:2010-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4230-4237.1995
更新日期:1995-08-01 00:00:00
abstract::Maintaining cell envelope integrity is critical for bacterial survival, including bacteria living in a complex and dynamic environment such as the human oral cavity. Streptococcus mutans, a major etiological agent of dental caries, uses two-component signal transduction systems (TCSTSs) to monitor and respond to vario...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01563-08
更新日期:2009-05-01 00:00:00
abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.446-452.1965
更新日期:1965-08-01 00:00:00
abstract::Rabbits immunized with mucopeptide derived from cell wall debris of Streptococcus agalactiae by use of the formamide extraction technique developed specific antibodies for bovine heart, skeletal muscle, lymph nodes, and blood buffy coat extracts. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.577-579.1967
更新日期:1967-02-01 00:00:00
abstract::The product of the papD gene of uropathogenic Escherichia coli is required for the biogenesis of digalactoside-binding P pili. Mutations within papD result in complete degradation of the major pilus subunit, PapA, and of the pilinlike proteins PapE and PapF and also cause partial breakdown of the PapG adhesin. The pap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6052-6058.1989
更新日期:1989-11-01 00:00:00
abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00876-13
更新日期:2013-10-01 00:00:00
abstract::Escherichia coli B dependence on CO2 for growth was demonstrated. At suboptimal CO2 concentrations the rate of growth was controlled by CO2 concentration. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.1162-1164.1978
更新日期:1978-09-01 00:00:00
abstract::Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1534-1542.2003
更新日期:2003-03-01 00:00:00
abstract::Edwards, P. R. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), R. Sakazaki, and I. Kato. Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima. J. Bacteriol. 84:99-103. 1962-Study of cultures of Salmonella mikawashima isolated from reptiles revealed that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.99-103.1962
更新日期:1962-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1026-1033.1971
更新日期:1971-12-01 00:00:00
abstract::The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7280-7284.1995
更新日期:1995-12-01 00:00:00
abstract::Chlamydia spp. exhibit a unique biphasic developmental cycle whereby infectious elementary bodies (EBs) invade host epithelial cells and differentiate into noninfectious, metabolically active reticulate bodies (RBs). EBs posses a unique outer envelope where rigidity is achieved by disulfide bonding among cysteine-rich...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05163-11
更新日期:2011-12-01 00:00:00
abstract::A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cros...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.2.596-603.1977
更新日期:1977-05-01 00:00:00
abstract::Mutations in two chromosomal genes of Escherichia coli, cpxA and cpxB, produced a temperature-sensitive growth defect that was remedied specifically by the addition of isoleucine and valine to the minimal medium. This auxotrophy was manifested only when the medium contained exogenous leucine, suggesting that mutant ce...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.68-73.1980
更新日期:1980-10-01 00:00:00
abstract::An inducible phosphoenolpyruvate:fructose phosphotransferase system has been detected in Azospirillum brasilense, which requires a minimum of two components of the crude extracts for activity: (i) a soluble fraction (enzyme I) and (ii) a membrane fraction (enzyme II). The uninduced cells neither show any uptake of fru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.1204-1206.1984
更新日期:1984-12-01 00:00:00
abstract::Previous microarray studies have suggested that an indirect mechanism of Fur regulation may be present in meningococcus at the posttranscriptional level through a small regulatory RNA (sRNA) system analogous to that of Escherichia coli and Pseudomonas aeruginosa. Recently, a Fur-regulated sRNA, NrrF, was identified th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00849-08
更新日期:2009-02-01 00:00:00
abstract::Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06414-11
更新日期:2012-03-01 00:00:00
abstract::plsB mutants of Escherichia coli are sn-glycerol 3-phosphate auxotrophs which owe their requirement to a K(m) defect in sn-glycerol 3-phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. We have located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.227-233.1974
更新日期:1974-10-01 00:00:00
abstract::Salmonella enterica encodes a type III secretion system within a pathogenicity island located at centisome 63 that is essential for virulence. All type III secretion systems require the function of a family of low-molecular-weight proteins that aid the secretion process by acting as partitioning factors and/or secreti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2262-2268.2000
更新日期:2000-04-01 00:00:00
abstract::An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.606-607.1970
更新日期:1970-10-01 00:00:00
abstract::Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis and consists of two extracellular proteins, BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids, 64.5 kDa) is a bacteriolytic peptidoglycan D-isoglutamyl-L-lysine endopeptidase that induces cell lysis of E. faecal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02203-14
更新日期:2015-01-01 00:00:00
abstract::Of 20 clinical isolates of Haemophilus parainfluenzae, 13 produced a mannose-resistant hemagglutinin that agglutinated erythrocytes from chickens, horses, rabbits, and sheep. Examination with the electron microscope showed that only strain HR-885 was pilate. Grown in static liquid culture, the 12 hemagglutinating, non...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.1075-1078.1981
更新日期:1981-02-01 00:00:00
abstract::The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.7.2528-2532.2006
更新日期:2006-04-01 00:00:00
abstract::Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.184-191.1967
更新日期:1967-07-01 00:00:00
abstract::A map location of the gluconate-6-phosphate dehydrogenase (gnd) marker was estimated in Escherichia coli C at approximately 46 min by P1 transduction. The gnd locus appears to lie between the co-transducible histidine and prophage P2 location I markers. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.1056-1058.1973
更新日期:1973-11-01 00:00:00
abstract::Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsatu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.22.6623-6627.1996
更新日期:1996-11-01 00:00:00
abstract::A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.831-836.1986
更新日期:1986-03-01 00:00:00
abstract::A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important poly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01321-06
更新日期:2006-12-01 00:00:00
abstract::Salmonella typhimurium contains three "major proteins" or "porins" (34K, 35K, and 36K) in the outer membrane. A mutant strain producing only the 35K porin was first grown in media containing high concentrations of NaCl to "repress" the porin synthesis and then was shifted into a medium without NaCl. The newly made por...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.687-702.1978
更新日期:1978-08-01 00:00:00