ssaD1, a suppressor of secA51(Ts) that renders growth of Escherichia coli cold sensitive, is an early amber mutation in the transcription factor gene nusB.

Abstract:

:Complementation analysis of the ssaD1 mutation, isolated as a suppressor of the secA51(Ts) mutation that renders growth of Escherichia coli cold sensitive, was used to show that ssaD corresponds to nusB, a gene known to be important in transcription antitermination. DNA sequence analysis of the ssaD1 allele showed that it creates an amber mutation in the 15th codon of nusB. Analysis of the effect of different levels of NusB protein on secA transcription and translation suggested that NusB plays little or no role in the control of secA expression. Accordingly, mechanisms by which nusB inactivation can lead to suppression of secA51(Ts) and secY24(Ts) mutations without affecting secA expression need to be considered.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rajapandi T,Oliver D

doi

10.1128/jb.176.14.4444-4447.1994

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

4444-7

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

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