Flagellin glycosylation island in Pseudomonas syringae pv. glycinea and its role in host specificity.

Abstract:

:The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To investigate the role of this posttranslational modification in the interactions between plants and bacterial pathogens, we isolated genes that are potentially involved in the posttranslational modification of flagellin in P. syringae pv. glycinea (glycosylation island); then defective mutants with mutations in these genes were generated. There are three open reading frames in the glycosylation island, designated orf1, orf2, and orf3. orf1 and orf2 encode putative glycosyltransferases, and mutants with defects in these open reading frames, deltaorf1 and deltaorf2, secreted nonglycosylated and slightly glycosylated flagellins, respectively. Inoculation tests performed with these mutants and original nonhost tobacco leaves revealed that deltaorf1 and deltaorf2 could grow on tobacco leaves and caused symptom-like changes. In contrast, these mutants failed to cause symptoms on original host soybean leaves. These data indicate that putative glycosyltransferases encoded in the flagellin glycosylation island are strongly involved in recognition by plants and could be the specific determinants of compatibility between phytopathogenic bacteria and plant species.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Takeuchi K,Taguchi F,Inagaki Y,Toyoda K,Shiraishi T,Ichinose Y

doi

10.1128/jb.185.22.6658-6665.2003

keywords:

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

6658-65

issue

22

eissn

0021-9193

issn

1098-5530

journal_volume

185

pub_type

杂志文章
  • A dominant-negative fur mutation in Bradyrhizobium japonicum.

    abstract::In many bacteria, the ferric uptake regulator (Fur) protein plays a central role in the regulation of iron uptake genes. Because iron figures prominently in the agriculturally important symbiosis between soybean and its nitrogen-fixing endosymbiont Bradyrhizobium japonicum, we wanted to assess the role of Fur in the i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.5.1409-1414.2004

    authors: Benson HP,LeVier K,Guerinot ML

    更新日期:2004-03-01 00:00:00

  • Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2.

    abstract::The complete nucleotide sequence of the linear plasmid pBD2 from Rhodococcus erythropolis BD2 comprises 210,205 bp. Sequence analyses of pBD2 revealed 212 putative open reading frames (ORFs), 97 of which had an annotatable function. These ORFs could be assigned to six functional groups: plasmid replication and mainten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.17.5269-5274.2003

    authors: Stecker C,Johann A,Herzberg C,Averhoff B,Gottschalk G

    更新日期:2003-09-01 00:00:00

  • Relationship between cell wall, cytoplasmic membrane, and bacterial motility.

    abstract::High-resolution electron microscopy of polarly flagellated bacteria revealed that their flagella originate at a circular, differentiated portion of the cytoplasmic membrane approximately 25 nm in diameter. The flagella also have discs attaching them to the cell wall. These attachment discs are extremely resistant to l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.512-521.1969

    authors: Vaituzis Z,Doetsch RN

    更新日期:1969-10-01 00:00:00

  • The amino acid sequence of Lrp is highly conserved in four enteric microorganisms.

    abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.6.1624-1626.1995

    authors: Friedberg D,Platko JV,Tyler B,Calvo JM

    更新日期:1995-03-01 00:00:00

  • Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281.

    abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4417-4425.1987

    authors: Jin SG,Komari T,Gordon MP,Nester EW

    更新日期:1987-10-01 00:00:00

  • The virulence regulator Sae of Staphylococcus aureus: promoter activities and response to phagocytosis-related signals.

    abstract::The two-component system SaeRS of Staphylococcus aureus is closely involved in the regulation of major virulence factors. However, little is known about the signals leading to saeRS activation. A total of four overlapping transcripts (T1 to T4) from three different transcription starting points are expressed in the sa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01927-07

    authors: Geiger T,Goerke C,Mainiero M,Kraus D,Wolz C

    更新日期:2008-05-01 00:00:00

  • Flagellar assembly in Salmonella typhimurium: analysis with temperature-sensitive mutants.

    abstract::The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.3.1327-1339.1990

    authors: Jones CJ,Macnab RM

    更新日期:1990-03-01 00:00:00

  • Glutamate transport in Escherichia coli K-12: nonidentity of carriers mediating entry and exit.

    abstract::The exit of glutamate from Escherichia coli K-12 cells preloaded with the radioactive amino acid and its relation to the reaction of entry were studied. Experiments with cells preloaded to different intracellular concentrations of radioactive glutamate confirmed our earlier conclusion that glutamate exit was a first-o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.51-57.1973

    authors: Halpern YS,Barash H,Druck K

    更新日期:1973-01-01 00:00:00

  • Complete genome sequence of Helicobacter cinaedi strain PAGU611, isolated in a case of human bacteremia.

    abstract::We report the complete genome sequence of Helicobacter cinaedi strain PAGU611, isolated in a case of human bacteremia. The PAGU611 genome comprises a 2,078,348-bp chromosome and a 23,054-bp plasmid. The chromosome contains a unique genomic island, encoding a type VI secretion system and clustered regularly interspaced...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00645-12

    authors: Goto T,Ogura Y,Hirakawa H,Tomida J,Morita Y,Akaike T,Hayashi T,Kawamura Y

    更新日期:2012-07-01 00:00:00

  • Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from Bacillus subtilis 168.

    abstract::Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.3.784-794.1974

    authors: Joenje H,Konings WN,Venema G

    更新日期:1974-09-01 00:00:00

  • Identification of Nudix hydrolase family members with an antimutator role in Mycobacterium tuberculosis and Mycobacterium smegmatis.

    abstract::Mycobacterium tuberculosis and Mycobacterium smegmatis MutT1, MutT2, MutT3, and Rv3908 (MutT4) enzymes were screened for an antimutator role. Results indicate that both MutT1, in M. tuberculosis and M. smegmatis, and MutT4, in M. smegmatis, have that role. Furthermore, an 8-oxo-guanosine triphosphatase function for Mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.3159-3161.2006

    authors: Dos Vultos T,Blázquez J,Rauzier J,Matic I,Gicquel B

    更新日期:2006-04-01 00:00:00

  • Escherichia coli NsrR regulates a pathway for the oxidation of 3-nitrotyramine to 4-hydroxy-3-nitrophenylacetate.

    abstract::Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PE...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00508-08

    authors: Rankin LD,Bodenmiller DM,Partridge JD,Nishino SF,Spain JC,Spiro S

    更新日期:2008-09-01 00:00:00

  • Cross-reactive antigen shared by Streptococcus agalactiae and certain bovine tissues.

    abstract::Rabbits immunized with mucopeptide derived from cell wall debris of Streptococcus agalactiae by use of the formamide extraction technique developed specific antibodies for bovine heart, skeletal muscle, lymph nodes, and blood buffy coat extracts. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.2.577-579.1967

    authors: Dodd K,Norcross NL

    更新日期:1967-02-01 00:00:00

  • Characterization of two tetracycline resistance determinants in Streptococcus faecalis JH1.

    abstract::Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugates obtained after mixed i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.835-843.1982

    authors: LeBlanc DJ,Lee LN

    更新日期:1982-05-01 00:00:00

  • Relation of catalase to substrate utilization by Mycoplasma pneumoniae.

    abstract::No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.4.1425-1430.1968

    authors: Low IE,Eaton MD,Proctor P

    更新日期:1968-04-01 00:00:00

  • The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases.

    abstract::The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.191-199.2002

    authors: Hoa NT,Brannigan JA,Cutting SM

    更新日期:2002-01-01 00:00:00

  • Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis.

    abstract::Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.4.793-796.1962

    authors: FUKUYAMA TT,O'KANE DJ

    更新日期:1962-10-01 00:00:00

  • Wide distribution of homologs of Escherichia coli Kdp K+-ATPase among gram-negative bacteria.

    abstract::We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.1192-1195.1989

    authors: Walderhaug MO,Litwack ED,Epstein W

    更新日期:1989-02-01 00:00:00

  • Cloning, sequence analysis, and characterization of the genes involved in isoprimeverose metabolism in Lactobacillus pentosus.

    abstract::Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.9.2312-2320.1998

    authors: Chaillou S,Lokman BC,Leer RJ,Posthuma C,Postma PW,Pouwels PH

    更新日期:1998-05-01 00:00:00

  • Heme and a five-amino-acid hemophore region form the bipartite stimulus triggering the has signaling cascade.

    abstract::Bacterial cells sense the extracellular environment and adapt to that environment by activating gene regulation circuits, often by means of signaling molecules. The Serratia marcescens hemophore is a signaling molecule that acts as an extracellular heme-scavenging protein. The heme-loaded hemophore interacts with its ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.9.3357-3364.2006

    authors: Cwerman H,Wandersman C,Biville F

    更新日期:2006-05-01 00:00:00

  • Regulation of Escherichia coli pyrC by the purine regulon repressor protein.

    abstract::The purine regulon repressor, PurR, was identified as a component of the Escherichia coli regulatory system for pyrC, the gene that encodes dihydroorotase, an enzyme in de novo pyrimidine nucleotide synthesis. PurR binds to a pyrC control site that resembles a pur regulon operator and represses expression by twofold. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3201-3207.1990

    authors: Choi KY,Zalkin H

    更新日期:1990-06-01 00:00:00

  • Dam methylation controls O-antigen chain length in Salmonella enterica serovar enteritidis by regulating the expression of Wzz protein.

    abstract::We reported previously that a Salmonella enterica serovar Enteritidis dam mutant expressing a truncated Dam protein does not agglutinate in the presence of specific antibodies against O9 polysaccharide. Here we investigate the participation of Dam in lipopolysaccharide (LPS) synthesis in Salmonella. The LPS O-antigen ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00839-09

    authors: Sarnacki SH,Marolda CL,Noto Llana M,Giacomodonato MN,Valvano MA,Cerquetti MC

    更新日期:2009-11-01 00:00:00

  • The caulobacter Tol-Pal complex is essential for outer membrane integrity and the positioning of a polar localization factor.

    abstract::Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00607-10

    authors: Yeh YC,Comolli LR,Downing KH,Shapiro L,McAdams HH

    更新日期:2010-10-01 00:00:00

  • VIRULENCE-LINKED COLONIAL AND MORPHOLOGICAL VARIATION IN LEPTOSPIRA.

    abstract::Faine, S. (University of Sydney, Sydney, Australia), and J. van der Hoeden. Virulence-linked colonial and morphological variation in Leptospira. J. Bacteriol. 88:1493-1496. 1964.-Large-colony typical hooked Leptospira icterohaemorrhagiae was virulent for hamsters and guinea pigs. On cultivation, it was gradually repla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1493-1496.1964

    authors: FAINE S,VANDERHOEDEN J

    更新日期:1964-11-01 00:00:00

  • Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis.

    abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4288-4296.1991

    authors: Stibitz S,Yang MS

    更新日期:1991-07-01 00:00:00

  • Formation of protoporphyrin from coproporphyrinogen in extracts of various bacteria.

    abstract::The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.398-403.1970

    authors: Jacobs NJ,Jacobs JM,Brent P

    更新日期:1970-05-01 00:00:00

  • Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties.

    abstract::Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. The purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. The enzyme contained 2 g-atoms of iron per mol of protein. The enzyme had a broa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.536-544.1976

    authors: Patel RN,Hou CT,Felix A,Lillard MO

    更新日期:1976-07-01 00:00:00

  • Molecular cloning and transcriptional analysis of a guanosine kinase gene of Brevibacterium acetylicum ATCC 953.

    abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.6959-6964.1997

    authors: Usuda Y,Kawasaki H,Shimaoka M,Utagawa T

    更新日期:1997-11-01 00:00:00

  • EslB Is Required for Cell Wall Biosynthesis and Modification in Listeria monocytogenes.

    abstract::Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00553-20

    authors: Rismondo J,Schulz LM,Yacoub M,Wadhawan A,Hoppert M,Dionne MS,Gründling A

    更新日期:2021-01-25 00:00:00

  • Photooxidative death in blue-green algae.

    abstract::When incubated in the light under 100% oxygen, wild-type blue-green algae (Anacystis nidulans, Synechococcus cedrorum) die out rapidly at temperatures of 4 to 15 C, and at 35 C (or at 26 C in the case of S. cedrorum) in the absence of CO(2). Photosynthesis is impaired in these cells long before they die. Blocking of p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.3.682-689.1972

    authors: Abeliovich A,Shilo M

    更新日期:1972-09-01 00:00:00