Uncontrolled septation in a cell division cycle mutant of the fission yeast Schizosaccharomyces pombe.

abstract：:The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by ...

journal_title：Journal of bacteriology

authors： Fouts KE,Barbour SD

更新日期：1982-01-01 00:00:00

abstract：:The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially composed of N-acetylglycosaminyl-N-acetylmuramic acid disaccharide units. Small amounts of N-acetylglycosaminyl-N-glyc...

journal_title：Journal of bacteriology

authors： Janczura E,Leyh-Bouille M,Cocito C,Ghuysen JM

更新日期：1981-02-01 00:00:00

abstract：:A genomic library containing HindIII partial digests of Rhodopseudomonas sphaeroides HR DNA was constructed in the broad-host-range cosmid cloning vector pVK102. With a portion of this library as donor in complementation studies with the ribulose 1,5-bisphosphate carboxylase-oxygenase regulatory mutant R. sphaeroides ...

journal_title：Journal of bacteriology

authors： Weaver KE,Tabita FR

更新日期：1985-10-01 00:00:00

abstract：:A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase,...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Rosenfeld HJ,Leifer Z,Maas WK

更新日期：1970-03-01 00:00:00

abstract：:Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. T...

journal_title：Journal of bacteriology

authors： Tam JE,Pattee PA

更新日期：1986-11-01 00:00:00

abstract：:The terminus region of the Caulobacter crescentus chromosome and the dif chromosome dimer resolution site were characterized. The Caulobacter genome contains skewed sequences that abruptly switch strands at dif and may have roles in chromosome maintenance and segregation. Absence of dif or the XerCD recombinase result...

journal_title：Journal of bacteriology

authors： Jensen RB

更新日期：2006-08-01 00:00:00

abstract：:The lethal activity of a thymineless mutant of Staphylococcus aureus Wood 46 strain has been compared with that of three naturally occurring strains: parent Wood 46, Smith, and coagulase-negative SA-13. The thymineless mutant and the parent Wood 46 strain showed a sharp decline in culturable units from the peritoneal ...

journal_title：Journal of bacteriology

authors： Mathieu LG,De Repentigny J,Turgeon S,Sonea S

更新日期：1967-07-01 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:The polycistronic mRNA encoding the nine genes of the unc operon of Escherichia coli was studied. We demonstrated the ribosome-binding capabilities of six of the nine unc genes, uncB, uncE, uncF, uncH, uncA, and uncD, by using the technique of primer extension inhibition or "toeprinting." No toeprint was detected for ...

journal_title：Journal of bacteriology

authors： Schaefer EM,Hartz D,Gold L,Simoni RD

更新日期：1989-07-01 00:00:00

abstract：:Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-bindin...

journal_title：Journal of bacteriology

authors： Abraham SN,Land M,Ponniah S,Endres R,Hasty DL,Babu JP

更新日期：1992-08-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:Campylobacter jejuni commensally colonizes the cecum of birds. The RacR (reduced ability to colonize) response regulator was previously shown to be important in avian colonization. To explore the means by which RacR and its cognate sensor kinase RacS may modulate C. jejuni physiology and colonization, ΔracR and ΔracS ...

journal_title：Journal of bacteriology

authors： Apel D,Ellermeier J,Pryjma M,Dirita VJ,Gaynor EC

更新日期：2012-05-01 00:00:00

abstract：:Among its penicillin-binding proteins (PBPs), Enterococcus faecium possesses a low-affinity PBP5, PBP5fm, which is the main target involved in beta-lactam resistance. A 7.7-kb EcoRI chromosomal fragment of E. faecium D63r containing the pbp5fm gene was cloned and sequenced. Two open reading frames (ORFs) were found. A...

journal_title：Journal of bacteriology

authors： Zorzi W,Zhou XY,Dardenne O,Lamotte J,Raze D,Pierre J,Gutmann L,Coyette J

更新日期：1996-08-01 00:00:00

abstract：:Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...

journal_title：Journal of bacteriology

authors： Engman J,Rogstam A,Frees D,Ingmer H,von Wachenfeldt C

更新日期：2012-03-01 00:00:00

abstract：:Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

journal_title：Journal of bacteriology

authors： Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

更新日期：2008-11-01 00:00:00

abstract：UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

journal_title：Journal of bacteriology

authors： Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

更新日期：2015-07-01 00:00:00

abstract：:Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic sulfur compounds. Here we present the draft genome sequence of Acidithiobacillus caldus ATCC 51756 (the type strain of the spe...

journal_title：Journal of bacteriology

authors： Valdes J,Quatrini R,Hallberg K,Dopson M,Valenzuela PD,Holmes DS

更新日期：2009-09-01 00:00:00

abstract：:Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...

journal_title：Journal of bacteriology

authors： Norlander L,Davies JK,Hagblom P,Normark S

更新日期：1981-02-01 00:00:00

abstract：:The genome of the facultative ribulose monophosphate (RuMP) cycle methylotroph Bacillus methanolicus encodes two bisphosphatases (GlpX), one on the chromosome (GlpX(C)) and one on plasmid pBM19 (GlpX(P)), which is required for methylotrophy. Both enzymes were purified from recombinant Escherichia coli and were shown t...

journal_title：Journal of bacteriology

authors： Stolzenberger J,Lindner SN,Persicke M,Brautaset T,Wendisch VF

更新日期：2013-11-01 00:00:00

abstract：:We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...

journal_title：Journal of bacteriology

authors： Drahos DJ,Hendrix RW

更新日期：1982-03-01 00:00:00

abstract：:In Selenomonas ruminantium, a strictly anaerobic and gram-negative bacterium, the degradation of lysine/ornithine decarboxylase (LDC/ODC) by ATP-requiring protease(s) is accelerated by the binding of P22, which is a ribosomal protein of this strain. Amino acid sequence alignment of S. ruminantium P22 with the L10 ribo...

journal_title：Journal of bacteriology

authors： Yamaguchi Y,Takatsuka Y,Kamio Y

更新日期：2008-01-01 00:00:00

abstract：:Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. The invasion process requires the synthesis and secretion of the virulence plasmid-encoded Ipa proteins. Using TnphoA mutagenesis, we have identified two virulence plasmid genes, mxiJ and mxiM, that encode proteins exporte...

journal_title：Journal of bacteriology

authors： Allaoui A,Sansonetti PJ,Parsot C

更新日期：1992-12-01 00:00:00

abstract：:Little is known about the effect of the host on the genetic stability of bacterial plant pathogens. Crown gall, a plant disease caused by Agrobacterium tumefaciens, may represent a useful model to study this effect. Indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria su...

journal_title：Journal of bacteriology

authors： Bélanger C,Canfield ML,Moore LW,Dion P

更新日期：1995-07-01 00:00:00

abstract：:Complementation analysis of the ssaD1 mutation, isolated as a suppressor of the secA51(Ts) mutation that renders growth of Escherichia coli cold sensitive, was used to show that ssaD corresponds to nusB, a gene known to be important in transcription antitermination. DNA sequence analysis of the ssaD1 allele showed tha...

journal_title：Journal of bacteriology

authors： Rajapandi T,Oliver D

更新日期：1994-07-01 00:00:00

abstract：:Transcription of the virG gene of Agrobacterium tumefaciens was previously shown to be expressed from two tandem promoters and to be responsive to three stimuli: plant-released phenolic compounds, phosphate starvation, and acidic media. In this report, I describe a set of deletions and other alterations of the 5' end ...

journal_title：Journal of bacteriology

authors： Winans SC

更新日期：1990-05-01 00:00:00

abstract：:The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. An rpoS mutant was examined under steady-state conditions with acetate as an electron donor and fumarate as an electron acceptor and with additional transcriptional ...

journal_title：Journal of bacteriology

authors： Núñez C,Esteve-Núñez A,Giometti C,Tollaksen S,Khare T,Lin W,Lovley DR,Methé BA

更新日期：2006-04-01 00:00:00

abstract：:Antibiotic resistance in Neisseria gonorrhoeae has been associated with the acquisition of R plasmids from heterologous organisms. The broad-host-range plasmids of incompatibility groups P (IncP) and Q (IncQ) have played a role in this genetic exchange in nature. We have utilized derivatives of RSF1010 (IncQ) and RP1 ...

journal_title：Journal of bacteriology

authors： Butler CA,Gotschlich EC

更新日期：1991-09-01 00:00:00

abstract：:The potent tumor-promoting agent croton oil, which has been shown previously to be strongly mitogenic in mammalian cells, stimulates cell division in snake mutants of the blue-green bacterium Agmenellum quadruplicatum. ...

journal_title：Journal of bacteriology

authors： Ingram LO,Fisher WD

更新日期：1973-05-01 00:00:00

abstract：:We used a Southern hybridization assay to locate precisely the sites at which DNA replication is arrested in the terminus region of the Escherichia coli chromosome. The assay was based on the properties of restriction fragments that contain stalled replication forks. Replication forks that entered the terminus from th...

journal_title：Journal of bacteriology

authors： Pelletier AJ,Hill TM,Kuempel PL

更新日期：1988-09-01 00:00:00

abstract：:Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...

journal_title：Journal of bacteriology

authors： Matsushita O,Russell JB,Wilson DB

更新日期：1990-07-01 00:00:00