Genetic evidence that the uvsE gene product of Deinococcus radiodurans R1 is a UV damage endonuclease.

Abstract:

:An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1(+) strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Earl AM,Rankin SK,Kim KP,Lamendola ON,Battista JR

doi

10.1128/jb.184.4.1003-1009.2002

keywords:

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

1003-9

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

184

pub_type

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