Abstract:
:An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1(+) strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Earl AM,Rankin SK,Kim KP,Lamendola ON,Battista JRdoi
10.1128/jb.184.4.1003-1009.2002keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
1003-9issue
4eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::The two highly homologous cbb operons of Alcaligenes eutrophus H16 that are located on the chromosome and on megaplasmid pHG1 contain genes encoding several enzymes of the Calvin carbon reduction cycle. Sequence analysis of a region from the promoter-distal part revealed two open reading frames, designated cbbT and cb...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7329-7340.1993
更新日期:1993-11-01 00:00:00
abstract::Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homolo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.1109-1114.1984
更新日期:1984-12-01 00:00:00
abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.), and J. H. Silliker. Food-poisoning potential of the enterococci. J. Bacteriol. 85:827-832. 1963.-A total of 23 enterococcus strains were fed to two and sometimes three human volunteers in an effort to elicit food-poisoning symptoms. Each culture was co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.827-832.1963
更新日期:1963-04-01 00:00:00
abstract::The fine structure of Paracoccidioides brasiliensis undergoing temperature-dependent transformation from mycelium to yeast and vice versa (M right harpoon over left harpoon Y) was studied. The transitional form to mycelium from the yeast appears as an elongated bud that extends from the yeast and which has a mixture o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1076-1082.1969
更新日期:1969-11-01 00:00:00
abstract::A number of heterologous plasmid deoxyribonucleic acids (DNAs) coding for erythromycin, tylosin, lincomycin, tetracycline, or chloramphenicol resistance have been introduced into Streptococcus pneumoniae via genetic transformation with frequencies that varied between 10(-5) to as high as 5 x 10(-1) per colony-forming ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.698-709.1980
更新日期:1980-11-01 00:00:00
abstract::Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.4056-4063.1990
更新日期:1990-07-01 00:00:00
abstract::The ftsZ gene of Escherichia coli, which lies in a cluster of cell division genes at 2 min on the genetic map, codes for a protein which is thought to play a key role in triggering cell division. Using an ftsZ::lacZ operon fusion, we have studied the transcription of the ftsZ gene under conditions in which cell divisi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1392-1399.1990
更新日期:1990-03-01 00:00:00
abstract::The growth cycle of Rickettsia quintana was studied for the first time in liquid culture. Growth of the microorganism in a transparent broth medium was made possible by the finding that fetal calf serum (FCS), but not calf serum (CS), satisfied the requirement of R. quintana (Fuller strain) for red blood cell lysate. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.1.184-190.1970
更新日期:1970-07-01 00:00:00
abstract:UNLABELLED:Streptococcus pneumoniae is able to integrate exogenous DNA into its genome by natural genetic transformation. Transient accumulation of high levels of the only S. pneumoniae alternative σ factor is insufficient for development of full competence without expression of a second competence-specific protein, Co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00354-16
更新日期:2016-08-11 00:00:00
abstract::In bacteria like Escherichia coli, the accumulation of glucose-6-phosphate (G6P) or its analogs such as α-methyl glucoside-6-phosphate (αMG6P) results in stress that appears in the form of growth inhibition. The small RNA SgrS is an essential part of the response that helps E. coli combat glucose-phosphate stress; the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00705-13
更新日期:2013-11-01 00:00:00
abstract::The products of the bvgAS locus coordinately regulate expression of the Bordetella pertussis virulence regulon in response to environmental signals. Transcription of bvgAS-activated genes is nearly eliminated by several modulating conditions, including the presence of sulfate anion or nicotinic acid and growth at low ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.970-979.1992
更新日期:1992-02-01 00:00:00
abstract::The dnaA operon of Escherichia coli contains the genes dnaA, dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF. When the DnaA concentration is raised, an increase in the number of DNA replication initiation events but a reduction in replication fork velocity occurs. Because DnaA is au...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.630-644.2003
更新日期:2003-01-01 00:00:00
abstract::A strain of Pseudomonas (P. putida HS1) was found to resemble P. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. The ability to degrade these substrates b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.952-964.1981
更新日期:1981-06-01 00:00:00
abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.299-302.1989
更新日期:1989-01-01 00:00:00
abstract::A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.862-870.1998
更新日期:1998-02-01 00:00:00
abstract::Genome comparisons have demonstrated that dramatic genetic change often underlies the emergence of new bacterial pathogens. Evolutionary analysis of Escherichia coli O157:H7, a pathogen that has emerged as a worldwide public health threat in the past two decades, has posited that this toxin-producing pathogen evolved ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.5.1783-1791.2005
更新日期:2005-03-01 00:00:00
abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.64-70.1969
更新日期:1969-10-01 00:00:00
abstract::Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 4...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.1.41-46.1972
更新日期:1972-04-01 00:00:00
abstract::The DNA binding proteins ArgR and AhrC are essential for regulation of arginine metabolism in Escherichia coli and Bacillus subtilis, respectively. A unique property of these regulators is that they form hexameric protein complexes, mediating repression of arginine biosynthetic pathways as well as activation of argini...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1147-1157.2004
更新日期:2004-02-01 00:00:00
abstract::We performed a systematic survey of transcription of a large region of the Rhizobium meliloti symbiotic plasmid pSym. This led to the discovery of two new sequences induced during symbiosis. The first sequence was linked to the known nitrogen fixation (nif-fix) gene cluster, and its expression depended on the nifA gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2239-2244.1987
更新日期:1987-05-01 00:00:00
abstract::The EcfG-type sigma factor RpoE2 is the regulator of the general stress response in Sinorhizobium meliloti. RpoE2 activity is negatively regulated by two NepR-type anti-sigma factors (RsiA1/A2), themselves under the control of two anti-anti-sigma factors (RsiB1/B2) belonging to the PhyR family of response regulators. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01623-14
更新日期:2014-07-01 00:00:00
abstract::Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) from Acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the Escherichia coli B enzyme. The molecular weights of the two native active enzymes as well as their alpha and beta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.435-443.1976
更新日期:1976-02-01 00:00:00
abstract::In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.11.3345-3353.1994
更新日期:1994-06-01 00:00:00
abstract::A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.867-875.1968
更新日期:1968-03-01 00:00:00
abstract::The product of the papD gene of uropathogenic Escherichia coli is required for the biogenesis of digalactoside-binding P pili. Mutations within papD result in complete degradation of the major pilus subunit, PapA, and of the pilinlike proteins PapE and PapF and also cause partial breakdown of the PapG adhesin. The pap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.6052-6058.1989
更新日期:1989-11-01 00:00:00
abstract::Nε-lysine acetylation was recently discovered on many bacterial proteins that function in diverse cellular processes. Thus, many questions remain unanswered. For example, what mechanisms regulate lysine acetylation? Does acetylation affect physiology? To help answer these questions, we studied the Escherichia coli res...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00383-13
更新日期:2013-09-01 00:00:00
abstract::Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00211-17
更新日期:2017-09-05 00:00:00
abstract::The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.4951-4958.1990
更新日期:1990-09-01 00:00:00
abstract::The synthesis of proteins necessary for the respiratory reduction of nitrate to dinitrogen is induced in most denitrifying bacteria by a shift to anaerobiosis. A homolog of the fur gene, which encodes a redox-active transcriptional activator in Escherichia coli, was isolated from Pseudomonas stutzeri by using the anr ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7236-7246.1993
更新日期:1993-11-01 00:00:00
abstract::Slein, Milton W. (Fort Detrick, Frederick, Md.) and Gerald F. Logan, Jr. Mechanism of action of the toxin of Bacillus anthracis. II. Alkaline phosphatasemia produced by culture filtrates of various bacilli. J. Bacteriol. 83:359-369. 1962.-A factor which produces hyperphosphatasemia after intravenous injection into ani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.359-369.1962
更新日期:1962-02-01 00:00:00