Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diaminopimelic acid adding enzyme.

Abstract:

:Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 42 C. NaCl had no influence on the Dpm adding enzyme activity in vitro. The growth rate of most temperature-resistant revertants was decreased, but their Dpm adding enzyme activity remained very low. Two revertants had a rather normal growth rate. Their Dpm adding enzyme activity was significantly increased, but much lower than in the wild type. The influence of growth rate on the viability of the mutants is discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lugtenberg EJ,v Schijndel-van Dam A

doi

10.1128/JB.110.1.41-46.1972

subject

Has Abstract

pub_date

1972-04-01 00:00:00

pages

41-6

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

110

pub_type

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