Abstract:
:Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-l-Ala- d-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 42 C. NaCl had no influence on the Dpm adding enzyme activity in vitro. The growth rate of most temperature-resistant revertants was decreased, but their Dpm adding enzyme activity remained very low. Two revertants had a rather normal growth rate. Their Dpm adding enzyme activity was significantly increased, but much lower than in the wild type. The influence of growth rate on the viability of the mutants is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lugtenberg EJ,v Schijndel-van Dam Adoi
10.1128/JB.110.1.41-46.1972subject
Has Abstractpub_date
1972-04-01 00:00:00pages
41-6issue
1eissn
0021-9193issn
1098-5530journal_volume
110pub_type
杂志文章abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1990-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00
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更新日期:1968-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.170.10.4522-4527.1988
更新日期:1988-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.683-690.1996
更新日期:1996-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1991-01-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00
abstract::The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.895-899.1985
更新日期:1985-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00