Regulation of spo0H, a gene coding for the Bacillus subtilis sigma H factor.

abstract：:The prfA gene of Listeria monocytogenes was recently reported to be required for expression of hly, which encodes a pore-forming hemolysin essential for pathogenicity (M. Leimeister-Wachter, C. Haffner, E. Domann, W. Goebel, and T. Chakraborty, Proc. Natl. Acad. Sci. USA 87:8336-8340, 1990). We demonstrate here that a...

journal_title：Journal of bacteriology

authors： Freitag NE,Youngman P,Portnoy DA

更新日期：1992-02-01 00:00:00

abstract：:Sensory adaptation by the chemotaxis system of Escherichia coli requires adjustments of the extent of methyl esterification of the chemotaxis receptor proteins. One mechanism utilized by E. coli to make such adjustments is to control the activity of CheB, the enzyme responsible for removing receptor methyl ester group...

journal_title：Journal of bacteriology

authors： Stewart RC,Roth AF,Dahlquist FW

更新日期：1990-06-01 00:00:00

abstract：:Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

journal_title：Journal of bacteriology

authors： Schrempf H,Bujard H,Hopwood DA,Goebel W

更新日期：1975-02-01 00:00:00

abstract：:Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS). Earlier studies indicated that an alternate glucose transport system functions in this organism under conditions of high growth rates, low pH, or excess glucose. To identify this system, S. mutans...

journal_title：Journal of bacteriology

authors： Cvitkovitch DG,Boyd DA,Thevenot T,Hamilton IR

更新日期：1995-05-01 00:00:00

abstract：:In recent work, we described the excision of a large genomic region from Enterococcus faecium D344R in which the sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of in...

journal_title：Journal of bacteriology

authors： Rice LB,Carias LL,Hutton-Thomas R,Rudin S

更新日期：2007-05-01 00:00:00

abstract：:Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...

journal_title：Journal of bacteriology

authors： Grodberg J,Dunn JJ

更新日期：1988-03-01 00:00:00

abstract：:Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

journal_title：Journal of bacteriology

authors： Brandriss MC

更新日期：1979-06-01 00:00:00

abstract：:Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble alpha-glucosidase (maltase) and flanking sequences from Sulfolobus solfa...

journal_title：Journal of bacteriology

authors： Rolfsmeier M,Haseltine C,Bini E,Clark A,Blum P

更新日期：1998-03-01 00:00:00

abstract：:Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative ...

journal_title：Journal of bacteriology

authors： DeCicco BT,Stukus PE

更新日期：1968-04-01 00:00:00

abstract：:Plasmid R1 is a low-copy-number plasmid that is present at a level of about four or five copies per average cell. The copy number is controlled posttranscriptionally at the level of synthesis of the rate-limiting initiator protein RepA. In addition to this, R1 has an auxiliary system that derepresses a second promoter...

journal_title：Journal of bacteriology

authors： Olsson JA,Paulsson J,Nordström K

更新日期：2004-01-01 00:00:00

abstract：:CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...

journal_title：Journal of bacteriology

authors： Johnson VG,Nicholls PJ

更新日期：1994-08-01 00:00:00

abstract：:Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilizatio...

journal_title：Journal of bacteriology

authors： Joshua CJ,Dahl R,Benke PI,Keasling JD

更新日期：2011-03-01 00:00:00

abstract：:Oceaniovalibus guishaninsula, as a representative of a new genus within the family Rhodobacteraceae, was isolated from surface seawater that was sulfidic. Here, we present the draft genome sequence of the type strain, JLT2003(T). ...

journal_title：Journal of bacteriology

authors： Tang K,Liu K,Jiao N

更新日期：2012-12-01 00:00:00

abstract：:The alpha-aminoadipate pathway for lysine biosynthesis is present only in fungi. The alpha-aminoadipate reductase (AAR) of this pathway catalyzes the conversion of alpha-aminoadipic acid to alpha-aminoadipic-delta-semialdehyde by a complex mechanism involving two gene products, Lys2p and Lys5p. The LYS2 and LYS5 genes...

journal_title：Journal of bacteriology

authors： Guo S,Evans SA,Wilkes MB,Bhattacharjee JK

更新日期：2001-12-01 00:00:00

abstract：:A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme ...

journal_title：Journal of bacteriology

authors： Morbidoni HR,de Mendoza D,Cronan JE Jr

更新日期：1996-08-01 00:00:00

abstract：:We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....

journal_title：Journal of bacteriology

authors： Condee CW,Summers AO

更新日期：1992-12-01 00:00:00

abstract：:Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...

journal_title：Journal of bacteriology

authors： Benachour A,Muller C,Dabrowski-Coton M,Le Breton Y,Giard JC,Rincé A,Auffray Y,Hartke A

更新日期：2005-02-01 00:00:00

abstract：:Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...

journal_title：Journal of bacteriology

authors： Tucker AN,White DC

更新日期：1970-08-01 00:00:00

abstract：:We are currently investigating the role of ToxR-mediated gene regulation in Photobacterium profundum strain SS9. SS9 is a moderately piezophilic ("pressure loving") psychrotolerant marine bacterium belonging to the family Vibrionaceae. In Vibrio cholerae, ToxR is a transmembrane DNA binding protein involved in mediati...

journal_title：Journal of bacteriology

authors： Bidle KA,Bartlett DH

更新日期：2001-03-01 00:00:00

abstract：:Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...

journal_title：Journal of bacteriology

authors： Ebisu S,Tsuboi A,Takagi H,Naruse Y,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1990-03-01 00:00:00

abstract：:A conditionally germination-defective mutant of Neurospora crassa has been found to be partially curable by ferricrocin and other siderophores. The mutant conidia rapidly lose their membrane-bound siderophores when suspended in buffer or growth media. Germination is consequently delayed unless large numbers of conidia...

journal_title：Journal of bacteriology

authors： Charlang G,Williams NP

更新日期：1977-12-01 00:00:00

abstract：:Dental plaque is a complex biofilm that accretes in a series of discrete steps proceeding from a gram-positive streptococcus-rich biofilm to a structure rich in gram-negative anaerobes. This study investigated information flow between two unrelated plaque bacteria, Streptococcus cristatus and Porphyromonas gingivalis....

journal_title：Journal of bacteriology

authors： Xie H,Cook GS,Costerton JW,Bruce G,Rose TM,Lamont RJ

更新日期：2000-12-01 00:00:00

abstract：:It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied un...

journal_title：Journal of bacteriology

authors： Fung JC,MacAlister TJ,Weigand RA,Rothfield LI

更新日期：1980-08-01 00:00:00

abstract：:Insertional mutagenesis was conducted on Bacillus subtilis cells to screen for mutants resistant to catabolite repression. Three classes of mutants that were resistant to glucose-promoted but not mannitol-promoted catabolite repression were identified. Cloning and sequencing of the mutated genes revealed that the muta...

journal_title：Journal of bacteriology

authors： Paulsen IT,Chauvaux S,Choi P,Saier MH Jr

更新日期：1998-02-01 00:00:00

abstract：:Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...

journal_title：Journal of bacteriology

authors： Choih SJ,Ferro AJ,Shapiro SK

更新日期：1977-07-01 00:00:00

abstract：:The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...

journal_title：Journal of bacteriology

authors： McBeth DL

更新日期：1989-02-01 00:00:00

abstract：:In Ralstonia eutropha H16, the nitric oxide (NO)-responsive transcriptional activator NorR controls the expression of a dicistronic operon that encodes a membrane-bound NO reductase, NorB, and a protein of unknown function, NorA. The N-terminal domain (NTD) of NorR is responsible for perception of the signal molecule,...

journal_title：Journal of bacteriology

authors： Klink A,Elsner B,Strube K,Cramm R

更新日期：2007-04-01 00:00:00

abstract：:Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated ...

journal_title：Journal of bacteriology

authors： Swenson PA

更新日期：1979-08-01 00:00:00

abstract：:The highly defective rho-15 mutant of Escherichia coli produces plasmid DNA that is 22% less negatively supercoiled than DNA from an isogenic wild-type strain (J. S. Fassler, G. F. Arnold, and I. Tessman, Mol. Gen. Genet. 204:424-429, 1986). We extended our measurements of plasmid superhelicity to additional rho mutan...

journal_title：Journal of bacteriology

authors： Arnold GF,Tessman I

更新日期：1988-09-01 00:00:00

abstract：:Rough variants of serovars from the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex were isolated in high frequency from pellicle growth of the wild-type strains. Rough morphology could be correlated with the lack of an outer cell wall sheath and its constituent C-mycoside glycopept...

journal_title：Journal of bacteriology

authors： Barrow WW,Brennan PJ

更新日期：1982-04-01 00:00:00