Characterization of the signaling domain of the NO-responsive regulator NorR from Ralstonia eutropha H16 by site-directed mutagenesis.

abstract：:Cytochrome bd oxidase operons from more than 50 species of bacteria contain a short gene encoding a small protein that ranges from ∼30 to 50 amino acids and is predicted to localize to the cell membrane. Although cytochrome bd oxidases have been studied for more than 70 years, little is known about the role of this sm...

journal_title：Journal of bacteriology

authors： VanOrsdel CE,Bhatt S,Allen RJ,Brenner EP,Hobson JJ,Jamil A,Haynes BM,Genson AM,Hemm MR

更新日期：2013-08-01 00:00:00

abstract：:The mechanisms protecting nitrogenase in Gluconacetobacter diazotrophicus from damage by oxygen were studied. Evidence is provided suggesting that in G. diazotrophicus these mechanisms include respiratory protection as well as conformational protection in which a putative FeSII Shethna protein is involved. ...

journal_title：Journal of bacteriology

authors： Ureta A,Nordlund S

更新日期：2002-10-01 00:00:00

abstract：:ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...

journal_title：Journal of bacteriology

authors： Berlyn MB,Giles NH

更新日期：1969-07-01 00:00:00

abstract：:The Escherichia coli strain cs2-68 is a cold-sensitive (c) mutant that forms a long filamentous cell at 20 degrees C with a large nucleoid mass in its central region. We have recently shown that the pcsA68 mutation causing the cs phenotype is a single-base substitution within the dinD gene, a DNA damage-inducible gene...

journal_title：Journal of bacteriology

authors： Yasuda T,Nagata T,Ohmori H

更新日期：1996-07-01 00:00:00

abstract：:Recently, a new type of K+ transporter, Ktr, has been identified in the bacterium Vibrio alginolyticus (T. Nakamura, R. Yuda, T. Unemoto, and E. P. Bakker, J. Bacteriol. 180:3491-3494, 1998). The Ktr transport system consists of KtrB, an integral membrane subunit, and KtrA, a subunit peripherally bound to the cytoplas...

journal_title：Journal of bacteriology

authors： Holtmann G,Bakker EP,Uozumi N,Bremer E

更新日期：2003-02-01 00:00:00

abstract：:Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...

journal_title：Journal of bacteriology

authors： Díaz-Celis C,Risca VI,Hurtado F,Polka JK,Hansen SD,Maturana D,Lagos R,Mullins RD,Monasterio O

更新日期：2017-09-05 00:00:00

abstract：:For cells of the yeast Saccharomyces cerevisiae, the size at initiation of budding is proportional to growth rate for rates from 0.33 to 0.23 h-1. At growth rates lower than 0.23 h-1, cells displayed a minimum cell size at bud initiation independent of growth rate. Regardless of growth rate, cells displayed an increas...

journal_title：Journal of bacteriology

authors： Johnston GC,Ehrhardt CW,Lorincz A,Carter BL

更新日期：1979-01-01 00:00:00

abstract：:We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...

journal_title：Journal of bacteriology

authors： Henriques AO,Bryan EM,Beall BW,Moran CP Jr

更新日期：1997-01-01 00:00:00

abstract：:A stereospecific enzyme activity capable of cleaving the amide bond of the synthetic substrate N-benzoyl-D-arginine-p-nitroanilide (D-BAPA) has been found in all aerobic and anaerobic members of the family Bacillaceae tested by us. Cells of nonsporeforming gram-positive or gram-negative bacteria contain a hydrolase ac...

journal_title：Journal of bacteriology

authors： Gofshtein-Gandman LV,Keynan A,Milner Y

更新日期：1988-12-01 00:00:00

abstract：:The emerging coverage of diverse habitats by metagenomic shotgun data opens new avenues of discovering functional novelty using computational tools. Here, we apply three different concepts for predicting novel functions within light-mediated microbial pathways in five diverse environments. Using phylogenetic approache...

journal_title：Journal of bacteriology

authors： Singh AH,Doerks T,Letunic I,Raes J,Bork P

更新日期：2009-01-01 00:00:00

abstract：:Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III ...

journal_title：Journal of bacteriology

authors： Burr SE,Stuber K,Wahli T,Frey J

更新日期：2002-11-01 00:00:00

abstract：:Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...

journal_title：Journal of bacteriology

authors： Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJ

更新日期：2008-11-01 00:00:00

abstract：:The bacteriophage P22-based challenge-phage system was used to study the binding of Xis and FIS to their sites in attP of bacteriophage lambda. Challenge phages were constructed that contained the X1, X2, and F sites within the P22 Pant promoter, which is required for expression of antirepressor. If Xis and FIS bind t...

journal_title：Journal of bacteriology

authors： Numrych TE,Gumport RI,Gardner JF

更新日期：1991-10-01 00:00:00

abstract：:Caulobacter crescentus initiates a single round of DNA replication during each cell cycle. Following the initiation of DNA replication, the essential CckA histidine kinase is activated by phosphorylation, which (via the ChpT phosphotransferase) enables the phosphorylation and activation of the CtrA global regulator. C...

journal_title：Journal of bacteriology

authors： Iniesta AA,Hillson NJ,Shapiro L

更新日期：2010-08-01 00:00:00

abstract：:Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...

journal_title：Journal of bacteriology

authors： Chen Y,Strain EA,Allard M,Brown EW

更新日期：2011-07-01 00:00:00

abstract：:Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the impo...

journal_title：Journal of bacteriology

authors： Rosey EL,Kennedy MJ,Petrella DK,Ulrich RG,Yancey RJ Jr

更新日期：1995-10-01 00:00:00

abstract：:The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...

journal_title：Journal of bacteriology

authors： Loroch AI,Nguyen BG,Ludwig RA

更新日期：1995-12-01 00:00:00

abstract：:The conditions for the infection of Bacillus stearothermophilus 4S with TP-1C phage deoxyribonucleic acid (DNA) are described. Cells from log-phase cultures are the most susceptible to phage DNA infection (transfection). A cellular component (competence factor) which enhances transfection is released into the culture ...

journal_title：Journal of bacteriology

authors： Streips UN,Welker NE

更新日期：1971-06-01 00:00:00

abstract：:We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced ...

journal_title：Journal of bacteriology

authors： Nørregaard-Madsen M,McFall E,Valentin-Hansen P

更新日期：1995-11-01 00:00:00

abstract：:The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...

journal_title：Journal of bacteriology

authors： Cooper S,Weinberger M

更新日期：1977-04-01 00:00:00

abstract：:Construction and characterization of a class of multicopy plasmid cloning vehicles containing the replication system of miniplasmid P15A are described. The constructed plasmids have cleavage sites within antibiotic resistance genes for a variety of commonly employed site-specific endonucleases, permitting convenient u...

journal_title：Journal of bacteriology

authors： Chang AC,Cohen SN

更新日期：1978-06-01 00:00:00

abstract：UNLABELLED:An ability to sense and respond to changes in extracellular phosphate is critical for the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation throug...

journal_title：Journal of bacteriology

authors： Lubin EA,Henry JT,Fiebig A,Crosson S,Laub MT

更新日期：2015-10-19 00:00:00

abstract：:The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

journal_title：Journal of bacteriology

authors： Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

更新日期：1975-11-01 00:00:00

abstract：:A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...

journal_title：Journal of bacteriology

authors： Warner JR,Morgan SA,Shulman RW

更新日期：1976-03-01 00:00:00

abstract：:The DNA sequence of a 2,100-bp region containing the argE gene from Escherichia coli has been determined. The nucleotide sequence of the ppc-argE intergenic region was also solved and shown to contain six tandemly repeated REP sequences. Moreover, the oxyR gene has been mapped on the E. coli chromosome and shown to fl...

journal_title：Journal of bacteriology

authors： Meinnel T,Schmitt E,Mechulam Y,Blanquet S

更新日期：1992-04-01 00:00:00

abstract：:The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant b...

journal_title：Journal of bacteriology

authors： Jackson DW,Suzuki K,Oakford L,Simecka JW,Hart ME,Romeo T

更新日期：2002-01-01 00:00:00

abstract：:When Legionella pneumophila grows on agar plates, it secretes a surfactant that promotes flagellum- and pilus-independent "sliding" motility. We isolated three mutants that were defective for surfactant. The first two had mutations in genes predicted to encode cytoplasmic enzymes involved in lipid metabolism. These ge...

journal_title：Journal of bacteriology

authors： Stewart CR,Burnside DM,Cianciotto NP

更新日期：2011-11-01 00:00:00

abstract：:Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding seque...

journal_title：Journal of bacteriology

authors： Horazdovsky BF,Hogg RW

更新日期：1989-06-01 00:00:00

abstract：:Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms. The E. coli che region was introduced into Salmonella recipients by means of a series of F-prime e...

journal_title：Journal of bacteriology

authors： DeFranco AL,Parkinson JS,Koshland DE Jr

更新日期：1979-07-01 00:00:00

abstract：:ParB is one of two P1-encoded proteins that are required for active partition of the P1 prophage in Escherichia coli. To probe the native domain structure of ParB, we performed limited proteolytic digestions of full-length ParB, as well as of several N-terminal and C-terminal deletion fragments of ParB. The C-terminal...

journal_title：Journal of bacteriology

authors： Surtees JA,Funnell BE

更新日期：1999-10-01 00:00:00