Abstract:
:The rates of DNA synthesis during the cell-division cycle were measured in Myxococcus xanthus growing in three different media permitting a twofold variation in doubling time. In all three media, simple DNA cycles were observed. Synthesis of DNA occurred during 85% of the cell-division cycle, independent of generation time, from 5 to 11 h. Cells were observed to contain one bacterial nucleoid at birth that later divided synchronously midway through the cell cycle. Nucleoid segregation appeared to begin before chromosome replication was completed. The DNA content of exponential-phase bacteria was determined to be about 20 +/- 3 X 10(-9) microgram per cell; newborn bacteria contained about 14 +/- 2 X 10(-9) microgram of DNA per cell. Exponential-phase bacteria showed about a 50% increase in DNA in the presence of chloramphenicol (50 microgram/ml). The number of randomly segregating chromosomes present in exponential-phase bacteria was determined by following the fate of prelabeled DNA during outgrowth in nonradioactive media. The results are consistent with a model in which cells are born with exactly one complete unreplicated chromosome. The molecular weight of such a chromosome is about 8.4 +/- 1.2 X 10(9).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zusman DR,Krotoski DM,Cumsky Mdoi
10.1128/JB.133.1.122-129.1978subject
Has Abstractpub_date
1978-01-01 00:00:00pages
122-9issue
1eissn
0021-9193issn
1098-5530journal_volume
133pub_type
杂志文章abstract::Alpha granules were isolated from a blue-green alga, Nostoc muscorum, in large quantities and high purity by sucrose density gradient centrifugation. Sodium deoxycholate was used to eliminate membrane contamination. Isolated alpha granules from this species have average dimension of 31 nm in width and 65 nm in length....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.331-338.1971
更新日期:1971-01-01 00:00:00
abstract:UNLABELLED:Biofilm formation is responsible for increased antibiotic tolerance in pathogenic bacteria. Cyclic di-GMP (c-di-GMP) is a widely used second-messenger signal that plays a key role in bacterial biofilm formation. c-di-GMP is synthesized by diguanylate cyclases (DGCs), a conserved class of enzymes absent in ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00742-15
更新日期:2015-09-28 00:00:00
abstract::Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated ou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2315-2322.1999
更新日期:1999-04-01 00:00:00
abstract::Type IIb heat-labile enterotoxin (LT-IIb) is produced by Escherichia coli 41. Restriction fragments of total cell DNA from strain 41 were cloned into a cosmid vector, and one cosmid clone that encoded LT-IIb was identified. The genes for LT-IIb were subcloned into a variety of plasmids, expressed in minicells, sequenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4945-4952.1989
更新日期:1989-09-01 00:00:00
abstract::Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.384-389.1985
更新日期:1985-10-01 00:00:00
abstract::Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.1117-1122.1978
更新日期:1978-06-01 00:00:00
abstract::The ability of Clostridium perfringens type A to produce an enterotoxin active in human food poisoning has been shown to be directly related to the ability of the organism to sporulate. Enterotoxin was produced only in a sporulation medium and not in a growth medium in which sporulation was repressed. Mutants with an ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.1.378-391.1972
更新日期:1972-04-01 00:00:00
abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.852-854.1985
更新日期:1985-05-01 00:00:00
abstract::Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the smooth-rough mutation in Salmonella minnesota. J. Bacteriol. 89:343-354. 1965.-A comparative study of the O antigen from the smoot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.2.343-354.1965
更新日期:1965-02-01 00:00:00
abstract::Two related killer strains of Saccharomyces cerevisiae were mutagenized and screened for nonkiller variants. About 20% of the mutants derived from one strain lacked all detectable double-straned ribonucleic acid (dsRNA). About 70% of the mutants from the other strain lacked one of the dsRNA species normally associated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.1.346-348.1977
更新日期:1977-10-01 00:00:00
abstract::Two nonconjugative R-plasmids, N-SuSm and N-Tc, have been characterized. Both were of relatively small size (5 x 10(6) to 6 x 10(6) daltons) and present in multiple copies within their respective bacterial hosts. N-SuSm possessed a guanine plus cytosine content of 55%, whereas N-Tc was 49% guanine plus cytosine. Altho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.619-630.1974
更新日期:1974-02-01 00:00:00
abstract::The exosporium of Bacillus anthracis is comprised of two distinct layers: a basal layer and a hair-like nap that covers the basal layer. The hair-like nap contains the glycoproteins BclA and, most likely, BclB. BclA and BclB are directed to assemble into the exosporium by motifs in their N-terminal domains. Here, we i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05658-11
更新日期:2011-10-01 00:00:00
abstract::The application of a simple negative staining technique and freeze-etching has revealed detailed information on the species-specific surface structure of spores and aerial hyphae of streptomycetes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.318-322.1970
更新日期:1970-01-01 00:00:00
abstract::During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5384-5388.1999
更新日期:1999-09-01 00:00:00
abstract::Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00390-06
更新日期:2006-08-01 00:00:00
abstract::The effects of guanosine tetraphosphate (ppGpp) and pyrimidine ribonucleoside triphosphates on Escherichia coli aspartate transcarbamylase (ATCase) synthesis were examined. To determine the effect of ppGpp, a stringent (relA+) and relaxed (relA) isogenic pair of E. coli K-12 strains was starved for isoleucine, and the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.998-1007.1983
更新日期:1983-02-01 00:00:00
abstract::Protoanemonin is a toxic metabolite which may be formed during the degradation of some chloroaromatic compounds, such as polychlorinated biphenyls, by natural microbial consortia. We show here that protoanemonin can be transformed by dienelactone hydrolase of Pseudomonas sp. strain B13 to cis-acetylacrylate. Although ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.2.400-402.1998
更新日期:1998-01-01 00:00:00
abstract::Transcriptomic, metabolomic, physiological, and computational modeling approaches were integrated to gain insight into the mechanisms of antibiotic tolerance in an in vitro biofilm system. Pseudomonas aeruginosa biofilms were grown in drip flow reactors on a medium composed to mimic the exudate from a chronic wound. A...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00307-19
更新日期:2019-10-21 00:00:00
abstract::The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1934-1939.1988
更新日期:1988-04-01 00:00:00
abstract::The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.15.4533-4539.1999
更新日期:1999-08-01 00:00:00
abstract::Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.1.219-223.1977
更新日期:1977-10-01 00:00:00
abstract::Anabaena variabilis ATCC 29413 has one Mo nitrogenase that is made under oxic growth conditions in specialized cells called heterocysts and a second Mo nitrogenase that is made only under anoxic conditions in vegetative cells. The two large nif gene clusters responsible for these two nitrogenases are under the control...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00674-16
更新日期:2017-01-30 00:00:00
abstract::The carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. Increasing concentrations of CO(2) were added as NaHCO(3) to a specially prepared CO(2)-free medium which was tubed and inoculated under nitrogen. Prior depletion of CO(2) in the inoculum was found...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.70-76.1971
更新日期:1971-01-01 00:00:00
abstract::Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, grown on methanol was found to show dihydroxyacetone synthase, dihydroxyacetone kinase, and ribulose 1,5-bisphosphate carboxylase, but no hydroxypyruvate reductase and very low hexulose 6-phosphate synthase, activities. The dihydroxyacetone synthase was foun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6041-6047.1997
更新日期:1997-10-01 00:00:00
abstract::Two nrdF genes, nrdF1 and nrdF2, encoding the small subunit (R2) of ribonucleotide reductase (RR) from Mycobacterium tuberculosis have 71% identity at the amino acid level and are both highly homologous with Salmonella typhimurium R2F. The calculated molecular masses of R2-1 and R2-2 are 36,588 (322 amino acids [aa]) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.20.6408-6415.1997
更新日期:1997-10-01 00:00:00
abstract::Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.536-540.1963
更新日期:1963-03-01 00:00:00
abstract::In an effort to identify the Agrobacterium tumefaciens phosphate regulatory gene(s), we isolated a clone from an A. tumefaciens cosmid library that restored regulated alkaline phosphatase activity to an Escherichia coli phoB mutant. The gene that complemented phoB was localized by subcloning and deletion analysis, and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6626-6636.1993
更新日期:1993-10-01 00:00:00
abstract::A colorimetric assay was developed for studying the kinetics of iron oxidation with whole cells of the chemoautotroph, Ferrobacillus ferrooxidans. The assay was more advantageous than the conventional method of Warburg manometry because of its simplicity, rapidity, and the small amount of cells required. The assay mea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.2.552-557.1969
更新日期:1969-08-01 00:00:00
abstract::The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.23-28.1984
更新日期:1984-04-01 00:00:00
abstract::We previously showed that the Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica (Y. Irie, S. Mattoo, and M. H. Yuk, J. Bacteriol. 186:5692-5698, 2004). Analyses of the extracellular components of B. bronchiseptica biofilm matrix revealed that the major sugar component in the matrix ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00605-06
更新日期:2006-09-01 00:00:00