Detoxification of protoanemonin by dienelactone hydrolase.

Abstract:

:Protoanemonin is a toxic metabolite which may be formed during the degradation of some chloroaromatic compounds, such as polychlorinated biphenyls, by natural microbial consortia. We show here that protoanemonin can be transformed by dienelactone hydrolase of Pseudomonas sp. strain B13 to cis-acetylacrylate. Although similar Km values were observed for cis-dienelactone and protoanemonin, the turnover rate of protoanemonin was only 1% that of cis-dienelactone. This indicates that at least this percentage of the enzyme is in the active state, even in the absence of activation. The trans-dienelactone hydrolase of Pseudomonas sp. strain RW10 did not detectably transform protoanemonin. Obviously, Pseudomonas sp. strain B13 possesses at least two mechanisms to avoid protoanemonin toxicity, namely a highly active chloromuconate cycloisomerase, which routes most of the 3-chloro-cis,cis-muconate to the cis-dienelactone, thereby largely preventing protoanemonin formation, and dienelactone hydrolase, which detoxifies any small amount of protoanemonin that might nevertheless be formed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Brückmann M,Blasco R,Timmis KN,Pieper DH

doi

10.1128/JB.180.2.400-402.1998

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

400-2

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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