Abstract:
:Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated outside but in close proximity to this transcriptional unit. The nahG and nahW genes of P. stutzeri AN10 are induced and expressed upon incubation with salicylate, and the enzymes that are encoded, NahG and NahW, are involved in naphthalene and salicylate metabolism. Both genes, nahG and nahW, have been cloned in Escherichia coli JM109. The overexpression of these genes yields peptides with apparent molecular masses of 46 kDa (NahG) and 43 kDa (NahW), respectively. Both enzymes exhibit broad substrate specificities and metabolize salicylate, methylsalicylates, and chlorosalicylates. However, the relative rates by which the substituted analogs are transformed differ considerably.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bosch R,Moore ER,García-Valdés E,Pieper DHdoi
10.1128/JB.181.8.2315-2322.1999keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
2315-22issue
8eissn
0021-9193issn
1098-5530journal_volume
181pub_type
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更新日期:1983-07-01 00:00:00
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更新日期:1997-12-01 00:00:00