NahW, a novel, inducible salicylate hydroxylase involved in mineralization of naphthalene by Pseudomonas stutzeri AN10.

Abstract:

:Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated outside but in close proximity to this transcriptional unit. The nahG and nahW genes of P. stutzeri AN10 are induced and expressed upon incubation with salicylate, and the enzymes that are encoded, NahG and NahW, are involved in naphthalene and salicylate metabolism. Both genes, nahG and nahW, have been cloned in Escherichia coli JM109. The overexpression of these genes yields peptides with apparent molecular masses of 46 kDa (NahG) and 43 kDa (NahW), respectively. Both enzymes exhibit broad substrate specificities and metabolize salicylate, methylsalicylates, and chlorosalicylates. However, the relative rates by which the substituted analogs are transformed differ considerably.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bosch R,Moore ER,García-Valdés E,Pieper DH

doi

10.1128/JB.181.8.2315-2322.1999

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

2315-22

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

杂志文章
  • A priA Mutant Expressed in Two Pieces Has Almost Full Activity in Escherichia coli K-12.

    abstract::The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00267-17

    authors: Leroux M,Jani N,Sandler SJ

    更新日期:2017-08-08 00:00:00

  • Evidence for two genetically distinct DNA primase activities specified by plasmids of the B and I incompatibility groups.

    abstract::Plasmid ColIb-P9 of the I alpha incompatibility group is known to encode a DNA primase that acts in the conjugal transfer of the plasmid and can substitute for mutant dnaG gene product in vegetative replication of the Escherichia coli chromosome. The relevant genetic determinant (sog) has previously been cloned into a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.1-7.1982

    authors: Dalrymple BP,Boulnois GJ,Wilkins BM,Orr E,Williams PH

    更新日期:1982-07-01 00:00:00

  • Characterization of exochelins of Mycobacterium avium: evidence for saturated and unsaturated and for acid and ester forms.

    abstract::Mycobacterium avium secretes iron-binding siderophores called exochelins. The exochelins from M. avium have previously been reported to have unsaturated side chains that terminate in carboxylic acid. In contrast, our data show the side chains to be both saturated and unsaturated and to terminate with either a carboxyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.21.6394-6398.1996

    authors: Wong DK,Gobin J,Horwitz MA,Gibson BW

    更新日期:1996-11-01 00:00:00

  • Identification of FkpA as a key quality control factor for the biogenesis of outer membrane proteins under heat shock conditions.

    abstract::The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01069-13

    authors: Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

    更新日期:2014-02-01 00:00:00

  • Francisella novicida CRISPR-Cas Systems Can Functionally Complement Each Other in DNA Defense while Providing Target Flexibility.

    abstract::CRISPR-Cas systems are prokaryotic adaptive immune systems that facilitate protection of bacteria and archaea against infection by external mobile genetic elements. The model pathogen Francisella novicida encodes a CRISPR-Cas12a (FnoCas12a) system and a CRISPR-Cas9 (FnoCas9) system, the latter of which has an addition...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00670-19

    authors: Ratner HK,Weiss DS

    更新日期:2020-05-27 00:00:00

  • Targeting the HUβ Protein Prevents Porphyromonas gingivalis from Entering into Preexisting Biofilms.

    abstract::The oral cavity is home to a wide variety of bacterial species, both commensal, such as various streptococcal species, and pathogenic, such as Porphyromonas gingivalis, one of the main etiological agents of periodontal disease. Our understanding of how these bacteria ultimately cause disease is highly dependent upon u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00790-17

    authors: Rocco CJ,Bakaletz LO,Goodman SD

    更新日期:2018-05-09 00:00:00

  • Genome sequence of Enterococcus sp. strain C1, an azo dye decolorizer.

    abstract::Enterococcus sp. strain C1 is a facultative anaerobe which was coisolated with Citrobacter sp. strain A1 from a sewage oxidation pond. Strain C1 could degrade azo dyes very efficiently via azo reduction and desulfonation in a microaerophilic environment. Here the draft genome sequence of Enterococcus sp. C1 is reporte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01372-12

    authors: Chan GF,Gan HM,Rashid NA

    更新日期:2012-10-01 00:00:00

  • Genetic control of the rate of alpha-amylase synthesis in Bacillus subtilis.

    abstract::The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.410-415.1974

    authors: Yamaguchi K,Nagata Y,Maruo B

    更新日期:1974-08-01 00:00:00

  • Reaction kinetics of substrate transglycosylation catalyzed by TreX of Sulfolobus solfataricus and effects on glycogen breakdown.

    abstract::We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc₄-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01442-13

    authors: Nguyen DH,Park JT,Shim JH,Tran PL,Oktavina EF,Nguyen TL,Lee SJ,Park CS,Li D,Park SH,Stapleton D,Lee JS,Park KH

    更新日期:2014-06-01 00:00:00

  • The concentration of ammonia regulates nitrogen metabolism in Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae was grown in a continuous culture at a single dilution rate with input ammonia concentrations whose effects ranged from nitrogen limitation to nitrogen excess and glucose limitation. The rate of ammonia assimilation (in millimoles per gram of cells per hour) was approximately constant. Increas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6672-6675.1995

    authors: ter Schure EG,Silljé HH,Verkleij AJ,Boonstra J,Verrips CT

    更新日期:1995-11-01 00:00:00

  • Natural and immune human antibodies reactive with antigens of virulent Neisseria gonorrhoeae: immunoglobulins G, M, And A.

    abstract::Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for huma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.141-148.1967

    authors: Cohen IR

    更新日期:1967-07-01 00:00:00

  • Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12.

    abstract::The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.127-134.1982

    authors: Gray JE,Wallen JW,Calhoun DH

    更新日期:1982-07-01 00:00:00

  • Cell growth inhibition upon deletion of four toxin-antitoxin loci from the megaplasmids of Sinorhizobium meliloti.

    abstract::Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01104-13

    authors: Milunovic B,diCenzo GC,Morton RA,Finan TM

    更新日期:2014-02-01 00:00:00

  • Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity.

    abstract::A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.21.6732-6741.1991

    authors: Rumbak E,Rawlings DE,Lindsey GG,Woods DR

    更新日期:1991-11-01 00:00:00

  • Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range.

    abstract::The hpaB gene encoding an aromatic hydroxylase of Escherichia coli ATCC 11105, a penicillin G acylase-producing strain, has been cloned and expressed in E. coli K-12. This gene was located near the pacA gene coding for penicillin G acylase. The hydroxylase has a molecular mass of 59,000 Da, uses NADH as a cosubstrate,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.2162-2167.1993

    authors: Prieto MA,Perez-Aranda A,Garcia JL

    更新日期:1993-04-01 00:00:00

  • Effect of pH and biotin on a circadian rhyythm of conidiation in Neurospora crassa.

    abstract::Expression of a circadian rhythm of conidiation in Neurospora crassa is enhanced in cultures grown on medium that is neutral or deficient in biotin. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.387-389.1975

    authors: West DJ

    更新日期:1975-07-01 00:00:00

  • Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.

    abstract::The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5343-5352.1987

    authors: Griggs DW,Tharp BB,Konisky J

    更新日期:1987-12-01 00:00:00

  • Role of the dinB gene product in spontaneous mutation in Escherichia coli with an impaired replicative polymerase.

    abstract::We isolated several new mutator mutations of the Escherichia coli replicative polymerase dnaE subunit alpha and used them and a previously reported dnaE mutation to study spontaneous frameshift and base substitution mutations. Two of these dnaE strains produce many more mutants when grown on rich (Luria-Bertani) than ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.23.6742-6750.2000

    authors: Strauss BS,Roberts R,Francis L,Pouryazdanparast P

    更新日期:2000-12-01 00:00:00

  • Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.

    abstract::Ruminococcin A (RumA) is a trypsin-dependent lantibiotic produced by Ruminococcus gnavus E1, a gram-positive strict anaerobic strain isolated from a human intestinal microbiota. A 12.8-kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumA, has been cloned and sequenced. It consisted ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.18-28.2002

    authors: Gomez A,Ladiré M,Marcille F,Fons M

    更新日期:2002-01-01 00:00:00

  • The stability region of the large virulence plasmid of Shigella flexneri encodes an efficient postsegregational killing system.

    abstract::The large virulence plasmid pMYSH6000 of Shigella flexneri contains a determinant that is highly effective in stabilizing otherwise unstable plasmids in Escherichia coli. Expression of two small contiguous genes, mvpA and mvpT (formerly termed STBORF1 and STBORF2), was shown to be sufficient for stability. Mutations i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.9.2416-2421.2000

    authors: Sayeed S,Reaves L,Radnedge L,Austin S

    更新日期:2000-05-01 00:00:00

  • Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12.

    abstract::Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.938-944.1998

    authors: Aono R,Tsukagoshi N,Yamamoto M

    更新日期:1998-02-01 00:00:00

  • Role of the electrochemical proton gradient in genetic transformation of Haemophilus influenzae.

    abstract::The uptake of homologous DNA by Haemophilus influenzae was studied as a function of the proton motive force in completely competent cultures in the pH range of 6 to 8. The composition and magnitude of the proton motive force were varied by using the ionophores valinomycin and nigericin (in the presence of various pota...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.868-873.1984

    authors: Bremer W,Kooistra J,Hellingwerf KJ,Konings WN

    更新日期:1984-03-01 00:00:00

  • Functionally critical elements of CooA-related CO sensors.

    abstract::CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Furth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.5.1320-1329.2004

    authors: Youn H,Kerby RL,Conrad M,Roberts GP

    更新日期:2004-03-01 00:00:00

  • Transfer of Rhizobium meliloti pSym genes into Agrobacterium tumefaciens: host-specific nodulation by atypical infection.

    abstract::The pSym megaplasmid of Rhizobium meliloti 2011 mobilized by plasmid RP4, or plasmid pGMI42, an RP4-prime derivative which carries a 290-kilobase pSym fragment including nitrogenase and nod genes, was introduced into Agrobacterium tumefaciens. The resulting transconjugants induced root deformations specifically on the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.1.134-142.1984

    authors: Truchet G,Rosenberg C,Vasse J,Julliot JS,Camut S,Denarie J

    更新日期:1984-01-01 00:00:00

  • The surfactant of Legionella pneumophila Is secreted in a TolC-dependent manner and is antagonistic toward other Legionella species.

    abstract::When Legionella pneumophila grows on agar plates, it secretes a surfactant that promotes flagellum- and pilus-independent "sliding" motility. We isolated three mutants that were defective for surfactant. The first two had mutations in genes predicted to encode cytoplasmic enzymes involved in lipid metabolism. These ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05405-11

    authors: Stewart CR,Burnside DM,Cianciotto NP

    更新日期:2011-11-01 00:00:00

  • Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes.

    abstract::A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complement...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6058-6065.1991

    authors: Cameron B,Guilhot C,Blanche F,Cauchois L,Rouyez MC,Rigault S,Levy-Schil S,Crouzet J

    更新日期:1991-10-01 00:00:00

  • HYDROLYSIS OF PROTEINS AND NUCLEIC ACIDS BY LANCEFIELD GROUP A AND OTHER STREPTOCOCCI.

    abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.). Hydrolysis of proteins and nucleic acids by Lancefield group A and other streptococci. J. Bacteriol. 86:1270-1274. 1963.-Classically, group A streptococci have been considered to be nonproteolytic in spite of the observation that a proteinase is produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1270-1274.1963

    authors: DEIBEL RH

    更新日期:1963-12-01 00:00:00

  • Restoration by ubiquinone of Azotobacter vinelandii reduced nicotinamide adenine dinucleotide oxidase activity.

    abstract::Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.311-313.1969

    authors: Swank RT,Burris RH

    更新日期:1969-04-01 00:00:00

  • Uric acid utilization by Mycobacterium intracellulare and Mycobacterium scrofulaceum isolates.

    abstract::Forty-nine human and environmental isolates of Mycobacterium intracellulare and Mycobacterium scrofulaceum were tested for their ability to grow on uric acid and a number of its degradation products. Nearly all (88 to 90%) strains used uric acid or allantoin as a sole nitrogen source; fewer (47 to 69%) used allantoate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.1.36-39.1983

    authors: Falkinham JO 3rd,George KL,Parker BC,Gruft H

    更新日期:1983-07-01 00:00:00

  • A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms.

    abstract::To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7360-7368.1997

    authors: Seow KT,Meurer G,Gerlitz M,Wendt-Pienkowski E,Hutchinson CR,Davies J

    更新日期:1997-12-01 00:00:00