Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli.

Abstract:

:The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene encoding a 32,000-dalton polypeptide was located downstream of and adjacent to cir, but did not appear to be part of the same transcriptional unit. A 525-base-pair fragment from the 5' end of the 1.8-kilobase-pair receptor-coding region directed iron-regulated transcription and translation of a hybrid cir-lacZ gene. Two overlapping promoters were identified by determination of the transcriptional start sites and by sequence analysis. A small open reading frame (120 nucleotides) of unknown significance preceded the receptor-coding sequence. Examination of the amino acid sequence of the receptor purified from the outer membrane revealed that the gene product was processed by removal of a signal peptide and that the mature form had an amino acid sequence near its amino terminus which closely resembled that of several other TonB-dependent proteins.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Griggs DW,Tharp BB,Konisky J

doi

10.1128/jb.169.12.5343-5352.1987

subject

Has Abstract

pub_date

1987-12-01 00:00:00

pages

5343-52

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

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