The concentration of ammonia regulates nitrogen metabolism in Saccharomyces cerevisiae.

Abstract:

:Saccharomyces cerevisiae was grown in a continuous culture at a single dilution rate with input ammonia concentrations whose effects ranged from nitrogen limitation to nitrogen excess and glucose limitation. The rate of ammonia assimilation (in millimoles per gram of cells per hour) was approximately constant. Increased extracellular ammonia concentrations are correlated with increased intracellular glutamate and glutamine concentrations, increases in levels of NAD-dependent glutamate dehydrogenase activity and its mRNA (gene GDH2), and decreases in levels of NADPH-dependent glutamate dehydrogenase activity and its mRNA (gene GDH1), as well as decreases in the levels of mRNA for the amino acid permease-encoding genes GAP1 and PUT4. The governing factor of nitrogen metabolism might be the concentration of ammonia rather than its flux.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

ter Schure EG,Silljé HH,Verkleij AJ,Boonstra J,Verrips CT

doi

10.1128/jb.177.22.6672-6675.1995

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

6672-5

issue

22

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

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