Functionally critical elements of CooA-related CO sensors.

Abstract:

:CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Further in vitro analysis of two CooA homologs from Azotobacter vinelandii and Carboxydothermus hydrogenoformans corroborated the in vivo data by revealing the ability of CO to bind to these hemoproteins and stimulate their binding at specific DNA sequences. These data, as well as the patterns of conserved residues in the homologs, are compared to what is already known about functionally important residues in the CooA protein of R. rubrum. The results identify critical regions of CooA and indicate features that distinguish CooAs from the general family of cyclic AMP receptor proteins.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Youn H,Kerby RL,Conrad M,Roberts GP

doi

10.1128/jb.186.5.1320-1329.2004

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

1320-9

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

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