Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12.

Abstract:

:Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that tolC is a member of the mar-sox regulon responsive to stress conditions. When a defective tolC gene was transferred to n-hexane- or cyclohexane-tolerant strains by P1 transduction, the organic solvent tolerance level was lowered dramatically to the decane-tolerant and nonane-sensitive level. The tolerance level was restored by transformation of the transductants with a wild-type tolC gene. Therefore, it is evident that TolC is essential for E. coli to maintain organic solvent tolerance.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Aono R,Tsukagoshi N,Yamamoto M

doi

10.1128/JB.180.4.938-944.1998

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

938-44

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

杂志文章
  • Functionally essential interaction between Yersinia YscO and the T3S4 domain of YscP.

    abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00876-13

    authors: Mukerjea R,Ghosh P

    更新日期:2013-10-01 00:00:00

  • Primary structure of the Escherichia coli serotype K42 capsular polysaccharide and its serological identity with the Klebsiella K63 polysaccharide.

    abstract::The Escherichia coli K42 capsular polysaccharide consists of leads to 3)-alpha-D-Galp-(1 leads to 3)-alpha-D-GalUAp-(1 leads to 3)-alpha-L-Fucp-(1 leads to repeating units. The E. coli K42 and Klebsiella K63 antigens are serologically identical. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.1.390-391.1978

    authors: Niemann H,Chakraborty AK,Friebolin H,Stirm S

    更新日期:1978-01-01 00:00:00

  • Characterization of the genes and proteins of a two-component system from the hyperthermophilic bacterium Thermotoga maritima.

    abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5579-5585.1996

    authors: Lee PJ,Stock AM

    更新日期:1996-10-01 00:00:00

  • The response of a Bacillus subtilis temperature-sensitive sigA mutant to heat stress.

    abstract::The mutant sigA allele of Bacillus subtilis DB1005 was confirmed to be temperature sensitive (ts) and transferable among strains of B. subtilis by chromosomal transformation and gene conversion. This ts sigA allele had a pleiotropic effect on gene expression of DB1005. The induction of certain heat shock proteins in D...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3102-3110.1994

    authors: Chang BY,Chen KY,Wen YD,Liao CT

    更新日期:1994-06-01 00:00:00

  • Relationship between sulfadiazine resistance and the failure to ferment maltose in Neisseria meningitidis.

    abstract::The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.3.557-561.1967

    authors: Kingsbury DT

    更新日期:1967-09-01 00:00:00

  • Regulation of formation of aerial mycelia and spores of Streptomyces viridochromogenes.

    abstract::Growth of Streptomyces viridochromogenes on a solid glycerol-NH4NO3 salts medium was accompanied by the formation of aerial mycelia and spores. Adding 0.5% or more casein hydrolysate to the medium stimulated growth while completely repressing the formation of aerial mycelia and spores. This repression was temporary, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1102-1111.1982

    authors: Coleman RH,Ensign JC

    更新日期:1982-03-01 00:00:00

  • An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima.

    abstract::Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.21.7267-7282.2005

    authors: Conners SB,Montero CI,Comfort DA,Shockley KR,Johnson MR,Chhabra SR,Kelly RM

    更新日期:2005-11-01 00:00:00

  • Noninvolvement of acyl carrier protein with citrate synthase and malate synthase.

    abstract::Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1281-1284.1968

    authors: Marcus SL,Reeves HC,Ajl SJ

    更新日期:1968-10-01 00:00:00

  • Isolation and characterization of mutants of colicin plasmids E1 and E2 after Mu bacteriophage infection.

    abstract::Cells colicinogenic for the colicin plasmids E1 or E2 (Col E1 and Col E2, respectively) were selected for a loss of colicin production after infection with bacteriophage Mu. Extrachromosomal deoxyribonucleic acid that was larger than the original colicin plasmids was found in such cells. A small insertion mutant in Co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.469-477.1974

    authors: Inselburg J

    更新日期:1974-08-01 00:00:00

  • Purification and properties of a serine protease from Pseudomonas matophilia.

    abstract::The extracellular protease of Pseudomonas maltophilia was partially purified by ammonium sulfate precipitation and chromatography on Sephadex G-75 and Bio-rex 70. Gel electrophoresis revealed minor impurities. The enzyme exhibited the following properties: (i) molecular weight, 35,000; (ii) A see article; 10.8; (iii) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.3.933-941.1975

    authors: Boethling RS

    更新日期:1975-03-01 00:00:00

  • Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.

    abstract::Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.479-489.1979

    authors: Linn T,Goman M,Scaife J

    更新日期:1979-11-01 00:00:00

  • Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus.

    abstract::We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.9.2711-2722.2003

    authors: Katayama Y,Takeuchi F,Ito T,Ma XX,Ui-Mizutani Y,Kobayashi I,Hiramatsu K

    更新日期:2003-05-01 00:00:00

  • Dosage of the smallest chromosome affects both the yeast-hyphal transition and the white-opaque transition of Candida albicans WO-1.

    abstract::The WO-1 strain of Candida albicans is capable of alternating between two highly distinct yeast cell types termed white and opaque (E. H. A. Rikkerrink, B. B. Magee, and P. T. Magee, J. Bacteriol. 170:895-899, 1988; B. Slutsky, M. Staebell, J. Anderson, L. Risen, M. Pfaller, and D. R. Soll, J. Bacteriol. 169:189-197, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7436-7442.1991

    authors: McEachern MJ,Hicks JB

    更新日期:1991-12-01 00:00:00

  • CATALASE ACTIVITY IN ANAPLASMA MARGINALE.

    abstract::Wallace, W. R. (Louisiana State University, Baton Rouge), and G. T. Dimopoullos. Catalase activity in Anaplasma marginale. J. Bacteriol. 91:309-311. 1965.-Extracts of erythrocytes infected with Anaplasma marginale were found to contain more catalase activity than normal erythrocytic preparations. The increase in catal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.309-311.1965

    authors: WALLACE WR,DIMOPOULLOS GT

    更新日期:1965-08-01 00:00:00

  • The RpoS sigma factor in the dissimilatory Fe(III)-reducing bacterium Geobacter sulfurreducens.

    abstract::Geobacter sulfurreducens RpoS sigma factor was shown to contribute to survival in stationary phase and upon oxygen exposure. Furthermore, a mutation in rpoS decreased the rate of reduction of insoluble Fe(III) but not of soluble forms of iron. This study suggests that RpoS plays a role in regulating metabolism of Geob...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.16.5543-5546.2004

    authors: Núñez C,Adams L,Childers S,Lovley DR

    更新日期:2004-08-01 00:00:00

  • Detection and quantification of superoxide formed within the periplasm of Escherichia coli.

    abstract::Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00554-06

    authors: Korshunov S,Imlay JA

    更新日期:2006-09-01 00:00:00

  • GENETIC INSTABILITY IN ESCHERICHIA COLI.

    abstract::Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) allel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.712-717.1965

    authors: SCHWARTZ NM

    更新日期:1965-03-01 00:00:00

  • Genomic sequence of C1, the first streptococcal phage.

    abstract::C(1), a lytic bacteriophage infecting group C streptococci, is one of the earliest-isolated phages, and the method of bacterial classification known as phage typing was defined by using this bacteriophage. We present for the first time a detailed analysis of this phage by use of electron microscopy, protein profiling,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.11.3325-3332.2003

    authors: Nelson D,Schuch R,Zhu S,Tscherne DM,Fischetti VA

    更新日期:2003-06-01 00:00:00

  • A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes.

    abstract::Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.5.1533-1540.1997

    authors: Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

    更新日期:1997-03-01 00:00:00

  • Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli.

    abstract::A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.350-355.1986

    authors: de Lorenzo V,Neilands JB

    更新日期:1986-07-01 00:00:00

  • Phospholipid biosynthesis in some anaerobic bacteria.

    abstract::We have identified and characterized enzymes of phospholipid synthesis in two plasmalogen-rich anaerobes. Megasphaera elsdenii and Veillonella parvula, and one anaerobe lacking plasmalogens. Desulfovibrio vulgaris. All three species contained phosphatidate cytidylyltransferase and phosphatidylserine synthase. Phosphat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.1.57-61.1981

    authors: Silber P,Borie RP,Mikowski EJ,Goldfine H

    更新日期:1981-07-01 00:00:00

  • Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.

    abstract::The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.20.6580-6589.1992

    authors: Godon JJ,Chopin MC,Ehrlich SD

    更新日期:1992-10-01 00:00:00

  • Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils.

    abstract::Haemophilus influenzae is a common gram-negative pathogen that initiates infection by colonizing the upper respiratory tract epithelium. In previous work, we reported the isolation of a locus involved in expression of short, thin surface fibrils by H. influenzae type b and presented evidence that surface fibrils promo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.21.6281-6287.1996

    authors: St Geme JW 3rd,Cutter D,Barenkamp SJ

    更新日期:1996-11-01 00:00:00

  • Genetic determinants of a nickel-specific transport system are part of the plasmid-encoded hydrogenase gene cluster in Alcaligenes eutrophus.

    abstract::Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.3.1340-1345.1989

    authors: Eberz G,Eitinger T,Friedrich B

    更新日期:1989-03-01 00:00:00

  • Purification of flagellar cores of Vibrio cholerae.

    abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.2.1121-1128.1977

    authors: Yang GC,Schrank GD,Freeman BA

    更新日期:1977-02-01 00:00:00

  • Extracellular Electron Transfer: Respiratory or Nutrient Homeostasis?

    abstract::Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens, such as Geobacter sulfurreducens and Shewanella oneidensis, are Gram negative. More recently, it has been reported that Gram-positive bacteria, such as L...

    journal_title:Journal of bacteriology

    pub_type: 评论,杂志文章

    doi:10.1128/JB.00029-20

    authors: Jeuken LJC,Hards K,Nakatani Y

    更新日期:2020-03-11 00:00:00

  • Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

    abstract::The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.21.6672-6676.1994

    authors: Kim SW,Kim CY,Benisek WF,Choi KY

    更新日期:1994-11-01 00:00:00

  • Characterization of nicotinamide mononucleotide adenylyltransferase from thermophilic archaea.

    abstract::The enzyme nicotinamide mononucleotide (NMN) adenylyltransferase (EC 2.7.7.1) catalyzes the synthesis of NAD+ and nicotinic acid adenine dinucleotide. It has been purified to homogeneity from cellular extracts of the thermophilic archaeon Sulfolobus solfataricus. Through a database search, a highly significant match w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.24.7718-7723.1997

    authors: Raffaelli N,Pisani FM,Lorenzi T,Emanuelli M,Amici A,Ruggieri S,Magni G

    更新日期:1997-12-01 00:00:00

  • Anaerobic electron transport in anaerobic flagellum formation in Escherichia coli.

    abstract::Flagellum formation by ubiquinone- and menaquinone-deficient mutant strains of Escherichia coli K-12 was studied under both aerobic and anaerobic growth conditions. Ubiquinone was found to be obligatory for aerobic flagellum formation but could be replaced by menaquinone for anaerobic flagellum formation. A mutant dev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.3.1034-1035.1977

    authors: Hertz R,Bar-Tana J

    更新日期:1977-12-01 00:00:00

  • Abbreviated Pathway for Biosynthesis of 2-Thiouridine in Bacillus subtilis.

    abstract:UNLABELLED:The 2-thiouridine (s(2)U) modification of the wobble position in glutamate, glutamine, and lysine tRNA molecules serves to stabilize the anticodon structure, improving ribosomal binding and overall efficiency of the translational process. Biosynthesis of s(2)U in Escherichia coli requires a cysteine desulfur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02625-14

    authors: Black KA,Dos Santos PC

    更新日期:2015-06-01 00:00:00