Utilization of nitrate by pathogenic and saprophytic mycobacteria.

Abstract:

:Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by Mycobacterium butyricum, M. smegmatis, and M. tuberculosis H37Ra. Nitrate and nitrite were both utilized as sole sources of nitrogen by M. tuberculosis H37Rv. The saprophytes and drug-resistant strains of tubercle bacilli failed to reduce nitrate after having been cultured repeatedly and stored in Kirchner medium. It was necessary to add molybdenum to Kirchner medium for continued nitrate reduction by the saprophytic mycobacteria, but not for M. tuberculosis. Nitrate reductase of M. tuberculosis was inhibited by tungstate; the inhibition was reversed by molybdate at a concentration of 1:10,000. Optimal conditions for the reduction of nitrate by washed whole cells and by a particulate sonictreated extract of M. tuberculosis were studied. Reduced diphosphopyridine nucleotide was required for reduction of nitrate by the cell extract. Nitrate reduction was inhibited by isoniazid but not by p-aminosalicylic acid or streptomycin.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

HEDGECOCK LW,COSTELLO RL

doi

10.1128/JB.84.2.195-205.1962

keywords:

subject

Has Abstract

pub_date

1962-08-01 00:00:00

pages

195-205

eissn

0021-9193

issn

1098-5530

journal_volume

84

pub_type

杂志文章
  • Residual thymidine incorporation in the presence of chloramphenicol in synchronous cultures of Escherichia coli B-r.

    abstract::Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.1.565-567.1974

    authors: Helmstetter CE

    更新日期:1974-10-01 00:00:00

  • F factor-mediated immunity to lethal zygosis in Escherichia coli K-12.

    abstract::Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.1.100-106.1974

    authors: Skurray RA,Reeves P

    更新日期:1974-01-01 00:00:00

  • Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp. Strain PCC 7120.

    abstract::HetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and he...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00707-17

    authors: Zhang H,Wang S,Wang Y,Xu X

    更新日期:2018-04-09 00:00:00

  • Further electron microscope characterization of spore appendages of Clostridium bifermentans.

    abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.231-238.1968

    authors: Yolton DP,Pope L,Williams MG,Rode LJ

    更新日期:1968-01-01 00:00:00

  • Molecular cloning of genes involved with expression of A-band lipopolysaccharide, an antigenically conserved form, in Pseudomonas aeruginosa.

    abstract::Most strains of Pseudomonas aeruginosa can express two chemically and immunologically distinct types of lipopolysaccharide (LPS), an antigenically conserved form called A band and the serotype-specific form called B band. To study the molecular controls regulating expression of the A-band LPS antigen, we have cloned t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5624-5630.1991

    authors: Lightfoot J,Lam JS

    更新日期:1991-09-01 00:00:00

  • Target analysis of mitochondrial genetic units in yeast.

    abstract::Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1142-1148.1969

    authors: Allen NE,MacQuillan AM

    更新日期:1969-03-01 00:00:00

  • Properties of an inducible C 4 -dicarboxylic acid transport system in Bacillus subtilis.

    abstract::The transport of the tricarboxylic acid cycle C(4)-dicarboxylic acids was studied in both the wild-type strain and tricarboxylic acid cycle mutants of Bacillus subtilis. Active transport of malate, fumarate, and succinate was found to be inducible by these dicarboxylic acids or by precursors to them, whereas glucose o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.1.65-79.1973

    authors: Ghei OK,Kay WW

    更新日期:1973-04-01 00:00:00

  • A Trigger Residue for Transmembrane Signaling in the Escherichia coli Serine Chemoreceptor.

    abstract:UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00274-15

    authors: Kitanovic S,Ames P,Parkinson JS

    更新日期:2015-08-01 00:00:00

  • INDUCTION OF NONSPECIFIC RESISTANCE BY ENDOTOXIN IN UNRESPONSIVE MICE.

    abstract::Margherita, S. S. (Temple University School of Medicine, Philadelphia, Pa.), and H. Friedmann. Induction of nonspecific resistance by endotoxin in unresponsive mice. J. Bacteriol. 89:277-280. 1965.-Serratia marcescens endotoxin was tested for protective effect in animals of varying immunological competence. A quantity...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.277-280.1965

    authors: MARGHERITA SS,FRIEDMAN H

    更新日期:1965-02-01 00:00:00

  • Primary structure of the wall peptidoglycan of leprosy-derived corynebacteria.

    abstract::The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially composed of N-acetylglycosaminyl-N-acetylmuramic acid disaccharide units. Small amounts of N-acetylglycosaminyl-N-glyc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.775-779.1981

    authors: Janczura E,Leyh-Bouille M,Cocito C,Ghuysen JM

    更新日期:1981-02-01 00:00:00

  • A nonswarming mutant of Proteus mirabilis lacks the Lrp global transcriptional regulator.

    abstract::Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a tight nonswarming mutant that did not elongate or upregulate flagellar and hemolysin genes. Individual cell motility was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.15.4741-4746.1997

    authors: Hay NA,Tipper DJ,Gygi D,Hughes C

    更新日期:1997-08-01 00:00:00

  • Myxococcus xanthus sasN encodes a regulator that prevents developmental gene expression during growth.

    abstract::Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Xu D,Yang C,Kaplan HB

    更新日期:1998-12-01 00:00:00

  • Repression of cytosine deaminase by pyrimidines in Salmonella typhimurium.

    abstract::The synthesis of cytosine deaminase in Salmonella typhimurium is repressed by pyrimidines. This repression is mediated by both a uridine and a cytidine compound, indicating a distinct difference in the regulation of synthesis of cytosine deaminase from the regulation of the de novo pyrimidine pathway enzymes. A salvag...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1171-1174.1982

    authors: West TP,O'Donovan GA

    更新日期:1982-03-01 00:00:00

  • Difference between manganese ion requirements of pediococci and enterococci.

    abstract::For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.627-628.1972

    authors: Efthymiou CJ,Joseph SW

    更新日期:1972-10-01 00:00:00

  • Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli.

    abstract::The genes controlling synthesis of the Escherichia coli acidic polysaccharide capsular antigens K10 and K54 were transferred by conjugation to E. coli strains of other serotypes. The genes concerned with these K antigen determinants showed genetic linkage with the serA locus. We propose to name the K antigen-controlli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.1.43-51.1974

    authors: Orskov I,Nyman K

    更新日期:1974-10-01 00:00:00

  • 3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product.

    abstract::The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.11.3632-3638.1997

    authors: Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

    更新日期:1997-06-01 00:00:00

  • Rabbit ileal loop response to strains of Clostridium perfringens.

    abstract::The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1560-1566.1968

    authors: Duncan CL,Sugiyama H,Strong DH

    更新日期:1968-05-01 00:00:00

  • Activation of spo0A transcription by sigma H is necessary for sporulation but not for competence in Bacillus subtilis.

    abstract::spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.12.3812-3815.1994

    authors: Siranosian KJ,Grossman AD

    更新日期:1994-06-01 00:00:00

  • A new type of Alcaligenes eutrophus CH34 zinc resistance generated by mutations affecting regulation of the cnr cobalt-nickel resistance system.

    abstract::Spontaneous mutants that were resistant to zinc were isolated from Alcaligenes eutrophus CH34 containing either the native plasmid pMOL28 or a derivative derepressed for its self-transfer, pMOL50. With the cured plasmid-free derivative of CH34, strain AE104, such mutants were not detected. The mutations, which were sh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.3.779-784.1993

    authors: Collard JM,Provoost A,Taghavi S,Mergeay M

    更新日期:1993-02-01 00:00:00

  • Instability of pathogenicity islands in uropathogenic Escherichia coli 536.

    abstract::The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled al...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.10.3086-3096.2004

    authors: Middendorf B,Hochhut B,Leipold K,Dobrindt U,Blum-Oehler G,Hacker J

    更新日期:2004-05-01 00:00:00

  • Formation of the K30 (group I) capsule in Escherichia coli O9:K30 does not require attachment to lipopolysaccharide lipid A-core.

    abstract::Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.23.7515-7522.1993

    authors: MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

    更新日期:1993-12-01 00:00:00

  • Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.

    abstract::Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.1.146-154.1979

    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

  • Protoplast transformation of glutamate-producing bacteria with plasmid DNA.

    abstract::A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the eff...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.306-311.1984

    authors: Katsumata R,Ozaki A,Oka T,Furuya A

    更新日期:1984-07-01 00:00:00

  • Mutational analysis of the TonB1 energy coupler of Pseudomonas aeruginosa.

    abstract::Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.6.1503-1513.2002

    authors: Zhao Q,Poole K

    更新日期:2002-03-01 00:00:00

  • Protein and fatty acid composition of mesosomal vesicles and plasma membranes of Staphylococcus aureus.

    abstract::Qualitatively, the protein and fatty acid composition of purified mesosomal vesicles and the plasma membrane isolated from Staphylococcus aureus ATCC 6538P are identical, the major difference between these two cellular components being only quantitative in nature. Mesosomal vesicles and plasma membranes, when subjecte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.571-576.1973

    authors: Theodore TS,Panos C

    更新日期:1973-11-01 00:00:00

  • Rhodobacter capsulatus OlsA is a bifunctional enzyme active in both ornithine lipid and phosphatidic acid biosynthesis.

    abstract::The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01121-07

    authors: Aygun-Sunar S,Bilaloglu R,Goldfine H,Daldal F

    更新日期:2007-12-01 00:00:00

  • Ribosomes, polyribosomes, and deoxyribonucleic acid from thermophilic mesophilic, and psychrophilic clostridia.

    abstract::Analysis of deoxyribonucleic acid (DNA) from four species of Clostridium, including two thermophiles, a mesophile, and a psychrophile, revealed no obvious relationship between growth temperature and DNA base composition. The melting temperatures (T(m)) of the DNA from the four species varied no more among the thermoph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.252-262.1973

    authors: Irwin CC,Akagi JM,Himes RH

    更新日期:1973-01-01 00:00:00

  • Reconstitution of pyrroloquinoline quinone-dependent D-glucose oxidase respiratory chain of Escherichia coli with cytochrome o oxidase.

    abstract::D-Glucose dehydrogenase is a pyrroloquinoline quinone-dependent primary dehydrogenase linked to the respiratory chain of a wide variety of bacteria. The enzyme exists in the membranes of Escherichia coli, mainly as an apoenzyme which can be activated by the addition of pyrroloquinoline quinone and magnesium. Thus, mem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.205-209.1987

    authors: Matsushita K,Nonobe M,Shinagawa E,Adachi O,Ameyama M

    更新日期:1987-01-01 00:00:00

  • Core and panmetabolism in Escherichia coli.

    abstract::Escherichia coli exhibits a wide range of lifestyles encompassing commensalism and various pathogenic behaviors which its highly dynamic genome contributes to develop. How environmental and host factors shape the genetic structure of E. coli strains remains, however, largely unknown. Following a previous study of E. c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01192-10

    authors: Vieira G,Sabarly V,Bourguignon PY,Durot M,Le Fèvre F,Mornico D,Vallenet D,Bouvet O,Denamur E,Schachter V,Médigue C

    更新日期:2011-03-01 00:00:00

  • Properties of 2-oxoglutarate:ferredoxin oxidoreductase from Thauera aromatica and its role in enzymatic reduction of the aromatic ring.

    abstract::Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.14.3975-3983.2002

    authors: Dörner E,Boll M

    更新日期:2002-07-01 00:00:00