Abstract:
:Hedgecock, L. W. (Veterans Administration Hospital, Kansas City, Mo.) and R. L. Costello. Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bacteriol. 84:195-205. 1962-The ability of mycobacteria to utilize nitrate as a sole source of nitrogen was examined. Nitrate-nitrogen was readily utilized by Mycobacterium butyricum, M. smegmatis, and M. tuberculosis H37Ra. Nitrate and nitrite were both utilized as sole sources of nitrogen by M. tuberculosis H37Rv. The saprophytes and drug-resistant strains of tubercle bacilli failed to reduce nitrate after having been cultured repeatedly and stored in Kirchner medium. It was necessary to add molybdenum to Kirchner medium for continued nitrate reduction by the saprophytic mycobacteria, but not for M. tuberculosis. Nitrate reductase of M. tuberculosis was inhibited by tungstate; the inhibition was reversed by molybdate at a concentration of 1:10,000. Optimal conditions for the reduction of nitrate by washed whole cells and by a particulate sonictreated extract of M. tuberculosis were studied. Reduced diphosphopyridine nucleotide was required for reduction of nitrate by the cell extract. Nitrate reduction was inhibited by isoniazid but not by p-aminosalicylic acid or streptomycin.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
HEDGECOCK LW,COSTELLO RLdoi
10.1128/JB.84.2.195-205.1962keywords:
subject
Has Abstractpub_date
1962-08-01 00:00:00pages
195-205eissn
0021-9193issn
1098-5530journal_volume
84pub_type
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doi:10.1128/JB.95.5.1560-1566.1968
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更新日期:1979-04-01 00:00:00
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更新日期:1984-07-01 00:00:00
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更新日期:2002-03-01 00:00:00
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doi:10.1128/JB.116.2.571-576.1973
更新日期:1973-11-01 00:00:00
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更新日期:1973-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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