Reconstitution of pyrroloquinoline quinone-dependent D-glucose oxidase respiratory chain of Escherichia coli with cytochrome o oxidase.

abstract：:The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

journal_title：Journal of bacteriology

authors： Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

更新日期：1993-04-01 00:00:00

abstract：:Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression of APase is regulated both negatively and positively. PhoP, a homolog of a family of bacterial transcription factors, and PhoR, a homolog of bacterial histidine pr...

journal_title：Journal of bacteriology

authors： Jensen KK,Sharkova E,Duggan MF,Qi Y,Koide A,Hoch JA,Hulett FM

更新日期：1993-06-01 00:00:00

abstract：:In the course of a study of genes located at min 44 of the Escherichia coli genome, we identified an open reading frame with the capacity to encode a 43-kDa polypeptide whose predicted amino acid sequence is strikingly similar to those of the well-known DD-carboxipeptidases penicillin-binding proteins PBP5 and PBP6. T...

journal_title：Journal of bacteriology

authors： Baquero MR,Bouzon M,Quintela JC,Ayala JA,Moreno F

更新日期：1996-12-01 00:00:00

abstract：:The buoyant density in rapidly growing Streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in Escherichia coli. Buoyant density in S. faecium was measured by using Percoll (Pharmacia Fine Chemicals, Piscataway, N.J.) density gradients. We found that the mean and coefficient of varia...

journal_title：Journal of bacteriology

authors： Glaser D,Higgins M

更新日期：1989-02-01 00:00:00

abstract：:Isolation of highly purified membrane fractions from phototrophically grown Rhodospirillum rubrum was achieved by velocity and isopyknic sedimentation under carefully controlled ionic conditions. Bacteriochlorophyll-rich and succinic dehydrogenase-rich chromatophores that were essentially devoid of contamination by no...

journal_title：Journal of bacteriology

authors： Collins ML,Niederman RA

更新日期：1976-06-01 00:00:00

abstract：:The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. py...

journal_title：Journal of bacteriology

authors： Odenbreit S,Wieland B,Haas R

更新日期：1996-12-01 00:00:00

abstract：:Dehalococcoides species are key players in the anaerobic transformation of halogenated solvents at contaminated sites. Here, we analyze isotopologue distributions in amino acid pools from peptides of Dehalococcoides strain CBDB1 after incubation with (13)C-labeled acetate or bicarbonate as a carbon source. The resulti...

journal_title：Journal of bacteriology

authors： Marco-Urrea E,Seifert J,von Bergen M,Adrian L

更新日期：2012-08-01 00:00:00

abstract：:Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...

journal_title：Journal of bacteriology

authors： Engman J,Rogstam A,Frees D,Ingmer H,von Wachenfeldt C

更新日期：2012-03-01 00:00:00

abstract：:Sulfolobus sulfataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryarchaeota and Crenarchaeota subdomains of the Archaea, contain proteins which are phosphorylated on tyrosine. These data raise fundamental questions as to the origin and evolution of tyrosine phosphorylati...

journal_title：Journal of bacteriology

authors： Smith SC,Kennelly PJ,Potts M

更新日期：1997-04-01 00:00:00

abstract：:Tetragenococcus halophila D10 catalyzes the decarboxylation of L-aspartate with nearly stoichiometric release of L-alanine and CO(2). This trait is encoded on a 25-kb plasmid, pD1. We found in this plasmid a putative asp operon consisting of two genes, which we designated aspD and aspT, encoding an L-aspartate-beta-de...

journal_title：Journal of bacteriology

authors： Abe K,Ohnishi F,Yagi K,Nakajima T,Higuchi T,Sano M,Machida M,Sarker RI,Maloney PC

更新日期：2002-06-01 00:00:00

abstract：:By extracellular labeling of peptides of intact Corynebacterium diphtheriae, followed by fractionation of the cells and chain completion by isolated polysomes, it is shown that diphtheria toxin is formed and secreted cotranslationally by membrane-bound polysomes; free polysomes from none. Moreover, when the chains on ...

journal_title：Journal of bacteriology

authors： Smith WP,Tai PC,Murphy JR,Davis BD

更新日期：1980-01-01 00:00:00

abstract：:Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium. ...

journal_title：Journal of bacteriology

authors： Robinson JP,Robinson RD,Hash JH

更新日期：1974-02-01 00:00:00

abstract：:XbaI digestion and pulsed-field gel electrophoresis of the genome of Salmonella typhimurium LT2 yields 24 fragments: 23 fragments (total size, 4,807 kb) are from the chromosome, and one fragment (90 kb) is from the virulence plasmid pSLT. Some of the 23 fragments from the chromosome were located on the linkage map by ...

journal_title：Journal of bacteriology

authors： Liu SL,Sanderson KE

更新日期：1992-03-01 00:00:00

abstract：:Chronic lung infections in cystic fibrosis (CF) could be treated more effectively if the effects of antimicrobials on pathogens in situ were known. Here, we compared changes in the microbial community composition and pathogen growth rates in longitudinal studies of seven pediatric CF patients undergoing intravenous an...

journal_title：Journal of bacteriology

authors： Neubauer C,Kasi AS,Grahl N,Sessions AL,Kopf SH,Kato R,Hogan DA,Newman DK

更新日期：2018-11-26 00:00:00

abstract：:The content and distribution of autolysin were measured in temperature-sensitive morphological mutants of Bacillus subtilis. Strains RUB1000 and RUB1012 grew as rods at 30 C. At 45 C the mutants contained disproportionately less teichoic acid than peptidoglycan and grew as irregular spheres. The amount of enzyme that ...

journal_title：Journal of bacteriology

authors： Brown WC,Wilson CR,Lukehart S,Young FE,Shiflett MA

更新日期：1976-01-01 00:00:00

abstract：:Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...

journal_title：Journal of bacteriology

authors： Donegan NP,Marvin JS,Cheung AL

更新日期：2014-12-01 00:00:00

abstract：:We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...

journal_title：Journal of bacteriology

authors： Batchelor RA,Haraguchi GE,Hull RA,Hull SI

更新日期：1991-09-01 00:00:00

abstract：:Streptococcus equi subsp. zooepidemicus is an opportunistic pathogen. It has caused a very large economic loss in the swine industry of China and has become a threat to human health. We announce the complete genome sequence of S. equi subsp. zooepidemicus strain ATCC 35246, which provides opportunities to understand i...

journal_title：Journal of bacteriology

authors： Ma Z,Geng J,Zhang H,Yu H,Yi L,Lei M,Lu CP,Fan HJ,Hu S

更新日期：2011-10-01 00:00:00

abstract：:The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD+, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for ener...

journal_title：Journal of bacteriology

authors： Westphal L,Wiechmann A,Baker J,Minton NP,Müller V

更新日期：2018-10-10 00:00:00

abstract：:Staphylococcus pseudintermedius is a member of the coagulase-positive staphylococci and is the commonest cause of canine pyoderma. We report the first genome sequence of S. pseudintermedius, which shows the presence of numerous virulence factors akin to those of the related human pathogen Staphylococcus aureus. ...

journal_title：Journal of bacteriology

authors： Tse H,Tsoi HW,Leung SP,Urquhart IJ,Lau SK,Woo PC,Yuen KY

更新日期：2011-04-01 00:00:00

abstract：:Plasmid pUB110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpC2 mutation in Bacillus subtilis from endonuclease EcoRI digested B. licheniformis, B. pumilus, and B. subtilis cellular deoxyribonucleic acid. Each of several such trp plasmids was subsequently shown to c...

journal_title：Journal of bacteriology

authors： Keggins KM,Lovett PS,Marrero R,Hoch SO

更新日期：1979-09-01 00:00:00

abstract：:Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...

journal_title：Journal of bacteriology

authors： Claus H,Friedrich A,Frosch M,Vogel U

更新日期：2000-03-01 00:00:00

abstract：:Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the ina...

journal_title：Journal of bacteriology

authors： Chandra TS,Friedrich CG

更新日期：1986-05-01 00:00:00

abstract：:LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...

journal_title：Journal of bacteriology

authors： Bowers CW,Lau F,Silhavy TJ

更新日期：2003-10-01 00:00:00

abstract：:Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugates obtained after mixed i...

journal_title：Journal of bacteriology

authors： LeBlanc DJ,Lee LN

更新日期：1982-05-01 00:00:00

abstract：:Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP):glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. As a result of the ptsG mutation, the apparent Km of the system for glucose transport is increased about 1,000...

journal_title：Journal of bacteriology

authors： Ruijter GJ,Postma PW,van Dam K

更新日期：1990-09-01 00:00:00

abstract：:Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms we...

journal_title：Journal of bacteriology

authors： Daumy GO,Danley D,McColl AS,Apostolakos D,Vinick FJ

更新日期：1985-09-01 00:00:00

abstract：:Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

journal_title：Journal of bacteriology

authors： Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

更新日期：2008-08-01 00:00:00

abstract：:The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods o...

journal_title：Journal of bacteriology

authors： Garnier M,Clerc M,Bové JM

更新日期：1984-04-01 00:00:00

abstract：:Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...

journal_title：Journal of bacteriology

authors： van der Ploeg J,Willemsen M,van Hall G,Janssen DB

更新日期：1995-03-01 00:00:00