Reconstitution of pyrroloquinoline quinone-dependent D-glucose oxidase respiratory chain of Escherichia coli with cytochrome o oxidase.

Abstract:

:D-Glucose dehydrogenase is a pyrroloquinoline quinone-dependent primary dehydrogenase linked to the respiratory chain of a wide variety of bacteria. The enzyme exists in the membranes of Escherichia coli, mainly as an apoenzyme which can be activated by the addition of pyrroloquinoline quinone and magnesium. Thus, membrane vesicles of E. coli can oxidize D-glucose to gluconate and generate an electrochemical proton gradient in the presence of pyrroloquinoline quinone. The D-glucose oxidase-respiratory chain was reconstituted into proteoliposomes, which consisted of two proteins purified from E. coli membranes, D-glucose dehydrogenase and cytochrome o oxidase, and E. coli phospholipids containing ubiquinone 8. The electron transfer rate during D-glucose oxidation and the membrane potential generation in the reconstituted proteoliposomes were almost the same as those observed in the membrane vesicles when pyrroloquinoline quinone was added. The results demonstrate that the quinoprotein, D-glucose dehydrogenase, can reduce ubiquinone 8 directly within phospholipid bilayer and that the D-glucose oxidase system of E. coli has a relatively simple respiratory chain consisting of primary dehydrogenase, ubiquinone 8, and a terminal oxidase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Matsushita K,Nonobe M,Shinagawa E,Adachi O,Ameyama M

doi

10.1128/jb.169.1.205-209.1987

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

205-9

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

杂志文章
  • Effects of local anesthetics on bacterial cells.

    abstract::The membrane effects of chlorpromazine, nupercain, tetracain, and procain were studied using Bacillus cereus, B. megaterium, B. subtilis, and Streptococcus faecalis, protoplasts from S. faecalis, and isolated membranes from B. subtilis. Chlorpromazin, nupercain, and tetracain produced characteristic micromorphological...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.461-468.1979

    authors: Silva MT,Sousa JC,Polónia JJ,Macedo PM

    更新日期:1979-01-01 00:00:00

  • Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.

    abstract::In Escherichia coli, the phosphorylation and dephosphorylation of isocitrate dehydrogenase (IDH) are catalyzed by a bifunctional protein kinase/phosphatase. We have determined the nucleotide sequence of aceK, the gene encoding IDH kinase/phosphatase. This gene consists of a single open reading frame of 1,734 base pair...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.6.2763-2769.1988

    authors: Klumpp DJ,Plank DW,Bowdin LJ,Stueland CS,Chung T,LaPorte DC

    更新日期:1988-06-01 00:00:00

  • Characterization of lipase-deficient mutants of Acinetobacter calcoaceticus BD413: identification of a periplasmic lipase chaperone essential for the production of extracellular lipase.

    abstract::Acinetobacter calcoaceticus BD413 produces an extracellular lipase, which is encoded by the lipA gene. Five lipase-deficient mutants have been generated via random insertion mutagenesis. Phenotypic characterization of these mutants revealed the presence of as many as four lipolytic enzymes in A. calcoaceticus. Biochem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.11.3295-3307.1995

    authors: Kok RG,van Thor JJ,Nugteren-Roodzant IM,Vosman B,Hellingwerf KJ

    更新日期:1995-06-01 00:00:00

  • MP13, a generalized transducing bacteriophage for Bacillus megaterium.

    abstract::The first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1112-1119.1982

    authors: Vary PS,Garbe JC,Franzen M,Frampton EW

    更新日期:1982-03-01 00:00:00

  • The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis.

    abstract::Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00485-08

    authors: Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJ

    更新日期:2008-11-01 00:00:00

  • Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I.

    abstract::Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.676-681.1972

    authors: Shizuya H,Dykhuizen D

    更新日期:1972-11-01 00:00:00

  • Cytochemical localization of catalase activity in yeast peroxisomes.

    abstract::Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.581-584.1970

    authors: Hoffmann HP,Szabo A,Avers CJ

    更新日期:1970-10-01 00:00:00

  • Interaction of Streptococcus mutans YidC1 and YidC2 with translating and nontranslating ribosomes.

    abstract::The YidC/OxaI/Alb3 family of membrane proteins is involved in the biogenesis of integral membrane proteins in bacteria, mitochondria, and chloroplasts. Gram-positive bacteria often contain multiple YidC paralogs that can be subdivided into two major classes, namely, YidC1 and YidC2. The Streptococcus mutans YidC1 and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00792-13

    authors: Wu ZC,de Keyzer J,Berrelkamp-Lahpor GA,Driessen AJ

    更新日期:2013-10-01 00:00:00

  • Vibrio cholerae H-NS silences virulence gene expression at multiple steps in the ToxR regulatory cascade.

    abstract::H-NS is an abundant nucleoid-associated protein involved in the maintenance of chromosomal architecture in bacteria. H-NS also has a role in silencing the expression of a variety of environmentally regulated genes during growth under nonpermissive conditions. In this study we demonstrate a role for H-NS in the negativ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.15.4295-4303.2000

    authors: Nye MB,Pfau JD,Skorupski K,Taylor RK

    更新日期:2000-08-01 00:00:00

  • The carboxy-terminal portion of the CheA kinase mediates regulation of autophosphorylation by transducer and CheW.

    abstract::The CheA kinase is a central protein in the signal transduction network that controls chemotaxis in Escherichia coli. CheA receives information from a transmembrane receptor (e.g., Tar) and CheW proteins and relays it to the CheB and CheY proteins. The biochemical activities of CheA proteins truncated at various dista...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.2097-2101.1993

    authors: Bourret RB,Davagnino J,Simon MI

    更新日期:1993-04-01 00:00:00

  • Effect of oxygen on liquid holding recovery of Bacteroides fragilis.

    abstract::Liquid holding recovery (LHR) in ultraviolet-irradiated Bacteroides fragilis cells occurred under aerobic conditions but was inhibited by anaerobic conditions. The increase in survival after aerobic LHR resulted in an increase in the shoulder regions of the ultraviolet survival curves. Maximum LHR was obtained after h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.1-7.1981

    authors: Jones DT,Woods DR

    更新日期:1981-01-01 00:00:00

  • Localization of acid phosphatase activity in the basidia of Coprinus micaceus.

    abstract::Gill lamellae from the young fruiting bodies of the basidiomycete Coprinus micaceus possess cytoplasmic particles with cytochemically demonstrable acid phosphatase activity; they are presumed to be lysosomes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.443-446.1973

    authors: Nehemiah JL

    更新日期:1973-07-01 00:00:00

  • Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui.

    abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.18.6198-6207.2004

    authors: Johnsen U,Schönheit P

    更新日期:2004-09-01 00:00:00

  • The Yersinia Yop virulon: LcrV is required for extrusion of the translocators YopB and YopD.

    abstract::LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.5.1207-1214.1998

    authors: Sarker MR,Neyt C,Stainier I,Cornelis GR

    更新日期:1998-03-01 00:00:00

  • Manganese, superoxide dismutase, and oxygen tolerance in some lactic acid bacteria.

    abstract::A previous study of the aerotolerant bacterium Lactobacillus plantarum, which lacks superoxide dismutase (SOD), demonstrated that it possesses a novel substitute for this defensive enzyme. Thus, L. plantarum contains 20 to 25 mM Mn(II),m in a dialyzable form, which is able to scavenge O2- apparently as effectively as ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.3.928-936.1981

    authors: Archibald FS,Fridovich I

    更新日期:1981-06-01 00:00:00

  • Structural diversity among methanofurans from different methanogenic bacteria.

    abstract::An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4594-4597.1988

    authors: White RH

    更新日期:1988-10-01 00:00:00

  • Structural and genetic analysis of X-ray scattering by spores of Bacillus subtilis.

    abstract::Dormant spores of Bacillus subtilis exhibit two prominent X-ray scattering peaks. These peaks persisted in spores lacking most alpha/beta-type small, acid-soluble protein or the CotE protein responsible for assembly of much spore coat protein, but they were absent from spores of strains lacking the late sporulation-sp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01200-09

    authors: Qiu X,Setlow P

    更新日期:2009-12-01 00:00:00

  • A Genome-Wide Helicobacter pylori Morphology Screen Uncovers a Membrane-Spanning Helical Cell Shape Complex.

    abstract::Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00724-18

    authors: Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

    更新日期:2019-06-21 00:00:00

  • Localization of c-di-GMP-binding protein with the linear terminal complexes of Acetobacter xylinum.

    abstract::Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.19.5668-5674.2001

    authors: Kimura S,Chen HP,Saxena IM,Brown RM Jr,Itoh T

    更新日期:2001-10-01 00:00:00

  • Importance of the E-46-D-160 polypeptide segment of the non-penicillin-binding module for the folding of the low-affinity, multimodular class B penicillin-binding protein 5 of Enterococus hirae.

    abstract::Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1774-1775.1996

    authors: Mollerach ME,Partoune P,Coyette J,Ghuysen JM

    更新日期:1996-03-01 00:00:00

  • Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores.

    abstract::Bacterial spores have long been recognized as the sturdiest known life forms on earth, revealing extraordinary resistance to a broad range of environmental assaults. A family of highly conserved spore-specific DNA-binding proteins, termed alpha/beta-type small, acid-soluble spore proteins (SASP), plays a major role in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.11.3525-3530.2004

    authors: Frenkiel-Krispin D,Sack R,Englander J,Shimoni E,Eisenstein M,Bullitt E,Horowitz-Scherer R,Hayes CS,Setlow P,Minsky A,Wolf SG

    更新日期:2004-06-01 00:00:00

  • Escherichia coli mutants thermosensitive for deoxyribonucleic acid gyrase subunit A: effects on deoxyribonucleic acid replication, transcription, and bacteriophage growth.

    abstract::Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.424-435.1979

    authors: Kreuzer KN,Cozzarelli NR

    更新日期:1979-11-01 00:00:00

  • Transcription patterns of Rhizobium meliloti symbiotic plasmid pSym: identification of nifA-independent fix genes.

    abstract::We performed a systematic survey of transcription of a large region of the Rhizobium meliloti symbiotic plasmid pSym. This led to the discovery of two new sequences induced during symbiosis. The first sequence was linked to the known nitrogen fixation (nif-fix) gene cluster, and its expression depended on the nifA gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.2239-2244.1987

    authors: David M,Domergue O,Pognonec P,Kahn D

    更新日期:1987-05-01 00:00:00

  • Homologous c-di-GMP-Binding Scr Transcription Factors Orchestrate Biofilm Development in Vibrio parahaemolyticus.

    abstract::The marine bacterium and human pathogen Vibrio parahaemolyticus rapidly colonizes surfaces by using swarming motility and forming robust biofilms. Entering one of the two colonization programs, swarming motility or sessility, involves differential regulation of many genes, resulting in a dramatic shift in physiology a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00723-19

    authors: Kimbrough JH,Cribbs JT,McCarter LL

    更新日期:2020-02-25 00:00:00

  • Pyrogenic principle of the ribonucleic acid from the yeast Candida utilis.

    abstract::Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.3.738-743.1968

    authors: Kanoh S,Kohlhage H,Siegert R

    更新日期:1968-09-01 00:00:00

  • Phosphoenolpyruvate-dependent maltose:phosphotransferase activity in Fusobacterium mortiferum ATCC 25557: specificity, inducibility, and product analysis.

    abstract::Phosphoenolypyruvate-dependent maltose:phosphotransferase activity was induced in cells of Fusobacterium mortiferum ATCC 25557 during growth on maltose. The disaccharide was rapidly metabolized by washed cells maintained under anaerobic conditions, but fermentation ceased immediately upon exposure of the cell suspensi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3250-3256.1994

    authors: Robrish SA,Fales HM,Gentry-Weeks C,Thompson J

    更新日期:1994-06-01 00:00:00

  • 2-keto-3-deoxygluconate, an intermediate in the fermentation of gluconate by clostridia.

    abstract::Five clostridial species were found to ferment gluconate via 2-keto-3-deoxygluconate which subsequently is phosphorylated to yield 2-keto-3-deoxy-6-phosphogluconate (KDPG). This compound is then cleaved by KDPG aldolase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.570-573.1971

    authors: Bender R,Andreesen JR,Gottschalk G

    更新日期:1971-08-01 00:00:00

  • Artificial septal targeting of Bacillus subtilis cell division proteins in Escherichia coli: an interspecies approach to the study of protein-protein interactions in multiprotein complexes.

    abstract::Bacterial cell division is mediated by a set of proteins that assemble to form a large multiprotein complex called the divisome. Recent studies in Bacillus subtilis and Escherichia coli indicate that cell division proteins are involved in multiple cooperative binding interactions, thus presenting a technical challenge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00462-08

    authors: Robichon C,King GF,Goehring NW,Beckwith J

    更新日期:2008-09-01 00:00:00

  • Regulation of Escherichia coli aspartate transcarbamylase synthesis by guanosine tetraphosphate and pyrimidine ribonucleoside triphosphates.

    abstract::The effects of guanosine tetraphosphate (ppGpp) and pyrimidine ribonucleoside triphosphates on Escherichia coli aspartate transcarbamylase (ATCase) synthesis were examined. To determine the effect of ppGpp, a stringent (relA+) and relaxed (relA) isogenic pair of E. coli K-12 strains was starved for isoleucine, and the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.2.998-1007.1983

    authors: Turnbough CL Jr

    更新日期:1983-02-01 00:00:00

  • Isolation of a novel protein involved in the transport of fructose by an inducible phosphoenolpyruvate fructose phosphotransferase system in Streptococcus mutans.

    abstract::Fructose transport in Streptococcus mutans LG-1 is mediated by at least two distinct phosphoenolpyruvate fructose phosphotransferase systems. One system is constitutive and consists of membrane components enzyme II as well as enzyme I and heat-stable protein. The other system is inducible and requires, in addition to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.2.755-763.1984

    authors: Gauthier L,Mayrand D,Vadeboncoeur C

    更新日期:1984-11-01 00:00:00