Analysis of autolysins in temperature-sensitive morphological mutants of Bacillus subtilis.

abstract：:The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...

journal_title：Journal of bacteriology

authors： Kennell D,Kotoulas A

更新日期：1967-01-01 00:00:00

abstract：:A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

journal_title：Journal of bacteriology

authors： Rastogi VK,Watson RJ

更新日期：1991-05-01 00:00:00

abstract：:Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...

journal_title：Journal of bacteriology

authors： Grogan DW,Rockwood J

更新日期：2010-09-01 00:00:00

abstract：:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

journal_title：Journal of bacteriology

authors： Bisercić M,Feutrier JY,Reeves PR

更新日期：1991-06-01 00:00:00

abstract：:Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosin...

journal_title：Journal of bacteriology

authors： Pester M,Brambilla E,Alazard D,Rattei T,Weinmaier T,Han J,Lucas S,Lapidus A,Cheng JF,Goodwin L,Pitluck S,Peters L,Ovchinnikova G,Teshima H,Detter JC,Han CS,Tapia R,Land ML,Hauser L,Kyrpides NC,Ivanova NN,Pagani

更新日期：2012-11-01 00:00:00

abstract：:The enzymatic properties and the physiological function of the type IV apurinic/apyrimidinic (AP)-endonuclease homolog of Bacillus subtilis, encoded by yqfS, a gene specifically expressed in spores, were studied here. To this end, a recombinant YqfS protein containing an N-terminal His6 tag was synthesized in Escheric...

journal_title：Journal of bacteriology

authors： Salas-Pacheco JM,Urtiz-Estrada N,Martínez-Cadena G,Yasbin RE,Pedraza-Reyes M

更新日期：2003-09-01 00:00:00

abstract：:Type 1 pili of Escherichia coli are the prototype of the somatic class of pili found on many strains of bacteria. As a first step in the genetic analysis of type 1 piliation, an extensive series of nonpiliated derivatives of E. coli K-12 strain AW405, was characterized to produce attached or free pili when examined in...

journal_title：Journal of bacteriology

authors： Swaney LM,Liu YP,To CM,To CC,Ippen-Ihler K,Brinton CC Jr

更新日期：1977-04-01 00:00:00

abstract：UNLABELLED:Biofilm formation is responsible for increased antibiotic tolerance in pathogenic bacteria. Cyclic di-GMP (c-di-GMP) is a widely used second-messenger signal that plays a key role in bacterial biofilm formation. c-di-GMP is synthesized by diguanylate cyclases (DGCs), a conserved class of enzymes absent in ma...

journal_title：Journal of bacteriology

authors： Fernicola S,Paiardini A,Giardina G,Rampioni G,Leoni L,Cutruzzolà F,Rinaldo S

更新日期：2015-09-28 00:00:00

abstract：:Clusters of cytoplasmic vesicles resembling those in growing hyphal apices of mycelial fungi are found near the tips of buds in three heterobasidiomycetous yeasts, Rhodotorula glutinis, Candida scottii, and Sporobolomyces salmonicolor. ...

journal_title：Journal of bacteriology

authors： McCully EK,Bracker CE

更新日期：1972-02-01 00:00:00

abstract：:Aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type II secretory pathway or secreton. ExeA is an essential component of this system and is necessary for the localization and/or multimerization of the secretin ExeD. ExeA contains two sequence motifs characteris...

journal_title：Journal of bacteriology

authors： Schoenhofen IC,Li G,Strozen TG,Howard SP

更新日期：2005-09-01 00:00:00

abstract：:Two activites causing nitrite disappearance are found in extracts of Neurospora; one, inducible by nitrate or nitrite and present only in nitrite-utilizing strains, catalyze the stoichiometric reduction of nitrite to ammonia; the other, present in all strains under all conditions, causes the disappearance of nitrite t...

journal_title：Journal of bacteriology

authors： Chang HC,Mulkins GJ,Dyer JC,Sorger GJ

更新日期：1975-08-01 00:00:00

abstract：UNLABELLED:Streptococcus pneumoniae is able to integrate exogenous DNA into its genome by natural genetic transformation. Transient accumulation of high levels of the only S. pneumoniae alternative σ factor is insufficient for development of full competence without expression of a second competence-specific protein, Co...

journal_title：Journal of bacteriology

authors： Tovpeko Y,Bai J,Morrison DA

更新日期：2016-08-11 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) quorum-sensing signaling is common to many Proteobacteria. Acyl-HSLs are synthesized by the LuxI family of synthases, and the signal response is mediated by members of the LuxR family of transcriptional regulators. Burkholderia thailandensis is a member of a closely related cluster o...

journal_title：Journal of bacteriology

authors： Chandler JR,Duerkop BA,Hinz A,West TE,Herman JP,Churchill ME,Skerrett SJ,Greenberg EP

更新日期：2009-10-01 00:00:00

abstract：:Bacterial lipases play important roles in bacterial metabolism and environmental response. Our laboratory recently discovered that a novel lipoprotein lysophospholipase, VolA, localizes on the surface of the Gram-negative aquatic pathogen Vibrio cholerae. VolA functions to cleave exogenous lysophosphatidylcholine, fre...

journal_title：Journal of bacteriology

authors： Pride AC,Guan Z,Trent MS

更新日期：2014-04-01 00:00:00

abstract：:Nicotinamide adenine dinucleotide (NAD) and reduced NAD (NADH) levels have been measured in bacterial cultures. The cofactors were assayed by using the very sensitive cycling assay described previously by Cartier. Control experiments showed that the level of total NAD(H) falls during harvesting, and so samples were ta...

journal_title：Journal of bacteriology

authors： Wimpenny JW,Firth A

更新日期：1972-07-01 00:00:00

abstract：:Partially purified inactive glucose dehydrogenase obtained from spores which were heated at 87 or 90 C for 30 min is converted to an active from by the addition of ethylenediaminetetraacetic acid, dipicolinic acid, or some salts. The molecular weight of the inactive glucose dehydrogenase in the heated spores is about ...

journal_title：Journal of bacteriology

authors： Hachisuka Y,Tochikubo K

更新日期：1971-08-01 00:00:00

abstract：:A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner foresp...

journal_title：Journal of bacteriology

authors： Germaine GR,Murrell WG

更新日期：1974-04-01 00:00:00

abstract：:The effects of various metabolic inhibitors on the motility of Spirochaeta aurantia were investigated. After 15 min in sodium arsenate buffer, 90% of cells remained motile even though adenosine triphosphate levels dropped from 5.6 to 0.1 nmol/mg (dry weight) of cells. After 70 min in sodium arsenate, 5% of cells were ...

journal_title：Journal of bacteriology

authors： Goulbourne EA Jr,Greenberg EP

更新日期：1980-09-01 00:00:00

abstract：:Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...

journal_title：Journal of bacteriology

authors： Price KE,Camilli A

更新日期：2009-04-01 00:00:00

abstract：:We detected inducible melibiose transport activity in cells of Enterobacter cloacae IID977. H+, but not Na+, was found to be the coupling cation for this transporter. We cloned and sequenced the gene encoding the melibiose transporter. A homology search of a protein sequence database revealed that this melibiose trans...

journal_title：Journal of bacteriology

authors： Okazaki N,Jue XX,Miyake H,Kuroda M,Shimamoto T,Tsuchiya T

更新日期：1997-07-01 00:00:00

abstract：:gldA, the structural gene for the NAD(+)-dependent glycerol dehydrogenase, was mapped at 89.2 min on the Escherichia coli linkage map, cotransducible with, but not adjacent to, the glpFKX operon encoding the proteins for the uptake and phosphorylation of glycerol. gldA was cloned, and its position on the physical map ...

journal_title：Journal of bacteriology

authors： Truniger V,Boos W

更新日期：1994-03-01 00:00:00

abstract：:Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (FeEnt) uptake through Escherichia coli FepA (EcoFepA). Among 165 inhibitors found...

journal_title：Journal of bacteriology

authors： Nairn BL,Eliasson OS,Hyder DR,Long NJ,Majumdar A,Chakravorty S,McDonald P,Roy A,Newton SM,Klebba PE

更新日期：2017-04-25 00:00:00

abstract：:We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

journal_title：Journal of bacteriology

authors： Lessner FH,Venters BJ,Keiler KC

更新日期：2007-01-01 00:00:00

abstract：:Bacteriophage phi29 protein p6 is a viral architectural protein, which binds along the whole linear phi29 DNA in vivo and is involved in initiation of DNA replication and transcription control. Protein p1 is a membrane-associated viral protein, proposed to attach the viral genome to the cell membrane. Protein p17 is i...

journal_title：Journal of bacteriology

authors： González-Huici V,Alcorlo M,Salas M,Hermoso JM

更新日期：2004-12-01 00:00:00

abstract：:The mechanisms by which Legionella pneumophila, a facultative intracellular parasite and the agent of Legionnaires' disease, acquires iron are largely unexplained. Several earlier studies indicated that L. pneumophila does not elaborate siderophores. However, we now present evidence that supernatants from L. pneumophi...

journal_title：Journal of bacteriology

authors： Liles MR,Scheel TA,Cianciotto NP

更新日期：2000-02-01 00:00:00

abstract：:Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread of genes when they are part of mobile cassettes. Most integrons are contained within chromosomes and are confined to specific bacte...

journal_title：Journal of bacteriology

authors： Stokes HW,Nesbø CL,Holley M,Bahl MI,Gillings MR,Boucher Y

更新日期：2006-08-01 00:00:00

abstract：:Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with (14)C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 mumole per hr per 10(8) cells and was mainly via the Embden-Meyerhof path...

journal_title：Journal of bacteriology

authors： Stjernholm RL

更新日期：1967-05-01 00:00:00

abstract：:ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...

journal_title：Journal of bacteriology

authors： Berlyn MB,Giles NH

更新日期：1969-07-01 00:00:00

abstract：:Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...

journal_title：Journal of bacteriology

authors： Camakaris J,Pittard J

更新日期：1971-08-01 00:00:00

abstract：:Under starvation conditions, a swarm of Myxococcus xanthus cells will undergo development, a multicellular process culminating in the formation of many aggregates called fruiting bodies, each of which contains up to 100,000 spores. The mechanics of symmetry breaking and the self-organization of cells into fruiting bod...

journal_title：Journal of bacteriology

authors： Zhang H,Angus S,Tran M,Xie C,Igoshin OA,Welch RD

更新日期：2011-10-01 00:00:00