Abstract:
:The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble products during a period of many hours. Rates of DNA synthesis were calculated by correcting the net amounts of DNA per milliliter to values that would have appeared had there been no decay. After the first few hours, this rate was constant, the amount of DNA present at the start of Mg(++) starvation being synthesized every 130 min. Rates of synthesis of total ribonucleic acid (RNA) were established in two ways: (i) by measurements of the incorporation of exogeneous uracil and glucose carbon into RNA, and (ii) by the accumulation of transfer RNA (tRNA), since this component is stable during Mg(++) starvation. After the first few hours, this rate was constant, the amount of RNA present at the start of Mg(++) starvation being synthesized about every 120 min. Fractionation by gradient centrifugation revealed that at all times of starvation the ratio of newly synthesized tRNA-rRNA was the same as it was during exponential growth. Furthermore, newly synthesized ribosomal RNA (rRNA) became a part of polysomal structures. Thus, in the absence of Mg(++), DNA, tRNA, and rRNA were synthesized in the same relative proportions as during exponential growth, at rates close to one-half the instantaneous rates of synthesis in the bacteria growing exponentially at the start of starvation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kennell D,Kotoulas Adoi
10.1128/JB.93.1.345-356.1967subject
Has Abstractpub_date
1967-01-01 00:00:00pages
345-56issue
1eissn
0021-9193issn
1098-5530journal_volume
93pub_type
杂志文章abstract::Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.210-216.1969
更新日期:1969-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.3.1322-1331.1983
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-11-01 00:00:00
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更新日期:2015-08-31 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.186.23.7980-7987.2004
更新日期:2004-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.3.1232-1238.1972
更新日期:1972-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
abstract::Wiley, W. R. (Washington State University, Pullman) and J. L. Stokes. Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. J. Bacteriol. 84:730-734. 1962-Resting-cell suspensions of Bacillus paseurii require a pH higher than 8.0 and also NH(4) (+) for the oxidation of glycine, alani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.730-734.1962
更新日期:1962-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.135.1.270-273.1978
更新日期:1978-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1775-1782.1988
更新日期:1988-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.22.7045-7054.1994
更新日期:1994-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.218-223.1990
更新日期:1990-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.583-588.1965
更新日期:1965-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.346-351.1968
更新日期:1968-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.3.1158-1164.1983
更新日期:1983-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2415-2423.1989
更新日期:1989-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.8.2200-2209.1994
更新日期:1994-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
abstract::After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.1.199-206.1970
更新日期:1970-07-01 00:00:00