Abstract:
:The cellular fatty acid composition of Mycobacterium vaccae JOB5 and Mycobacterium convolutum R22 was examined after growth on n-alkanes and compared with the fatty acids of the organisms after growth on 1-chlorohexadecane and 1-chlorooctadecane. Growth on n-alkanes resulted in normal fatty acid profiles. Mass spectral analyses indicated that, after growth on the terminally chlorinated n-alkanes, 75 to 86% of the fatty acids in M. convolutum and ca. 55% of the fatty acids in M. vaccae contained chlorine. Neither organism could utilize chloroacetate or 3-chloropropionate as sole source of carbon and energy. When these compounds were added to a growth medium with n-hexadecane as substrate, there was no evidence that chlorinated fatty acids were produced. Terminally chlorinated n-alkanes can be added to the list of n-alkanes, alkenes, and cyclohexylalkane derivatives that can be directly incorporated into cellular fatty acids of hydrocarbon-utilizing organisms.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Murphy GL,Perry JJdoi
10.1128/JB.156.3.1158-1164.1983subject
Has Abstractpub_date
1983-12-01 00:00:00pages
1158-64issue
3eissn
0021-9193issn
1098-5530journal_volume
156pub_type
杂志文章abstract::A search by subtractive hybridization for sequences present in only certain strains of Helicobacter pylori led to the discovery of a 2-kb transposable element to be called IS607, which further PCR and hybridization tests indicated was present in about one-fifth of H. pylori strains worldwide. IS607 contained two open ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.19.5300-5308.2000
更新日期:2000-10-01 00:00:00
abstract::Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.10.2583-2589.1998
更新日期:1998-05-01 00:00:00
abstract::Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7378-7389.2004
更新日期:2004-11-01 00:00:00
abstract::Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.404-410.1970
更新日期:1970-05-01 00:00:00
abstract::Two key elements that are thought to be required for replication initiation in eubacteria are the DnaA protein, a trans-acting factor, and the replication origin, a cis-acting element. As a first step in studying the replication initiation process in mycobacteria, we have isolated a 4-kb chromosomal DNA fragment from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.22.6527-6535.1995
更新日期:1995-11-01 00:00:00
abstract::Geobacter sulfurreducens RpoS sigma factor was shown to contribute to survival in stationary phase and upon oxygen exposure. Furthermore, a mutation in rpoS decreased the rate of reduction of insoluble Fe(III) but not of soluble forms of iron. This study suggests that RpoS plays a role in regulating metabolism of Geob...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.16.5543-5546.2004
更新日期:2004-08-01 00:00:00
abstract::The deletion of a gene coding for a histidine kinase (sll0750, Hik8) in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 resulted in a conditional lethal phenotype with a pleiotropic effect on the expression of genes involved in glucose metabolism. This mutant had comparable doubling times to wild type...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2368-2376.2005
更新日期:2005-04-01 00:00:00
abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3872-3880.1989
更新日期:1989-07-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system (T3SS) to evade phagocytosis and damage eukaryotic cells. Transcription of the T3SS regulon is controlled by ExsA, a member of the AraC/XylS family of transcriptional regulators. These family members generally consist of an approxim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00002-09
更新日期:2009-06-01 00:00:00
abstract::Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.24.7397-7402.2001
更新日期:2001-12-01 00:00:00
abstract::In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.7.1741-1749.1998
更新日期:1998-04-01 00:00:00
abstract::pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4809-4812.1995
更新日期:1995-08-01 00:00:00
abstract::The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00970-10
更新日期:2011-01-01 00:00:00
abstract::In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.131-135.1982
更新日期:1982-01-01 00:00:00
abstract::In Selenomonas ruminantium, a strictly anaerobic and gram-negative bacterium, the degradation of lysine/ornithine decarboxylase (LDC/ODC) by ATP-requiring protease(s) is accelerated by the binding of P22, which is a ribosomal protein of this strain. Amino acid sequence alignment of S. ruminantium P22 with the L10 ribo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01429-07
更新日期:2008-01-01 00:00:00
abstract::Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00959-09
更新日期:2010-02-01 00:00:00
abstract::Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01848-07
更新日期:2008-06-01 00:00:00
abstract::Formation of the Escherichia coli division septum is catalyzed by a number of essential proteins (named Fts) that assemble into a ring-like structure at the future division site. Many of these Fts proteins are intrinsic transmembrane proteins whose functions are largely unknown. In the present study, we attempted to i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00331-08
更新日期:2009-01-01 00:00:00
abstract::Methylation of DNA at the C-5 position of cytosine occurs in diverse organisms. This modification can increase the rate of C→T transitions at the methylated position. In Escherichia coli and related enteric bacteria, the inner C residues of the sequence CCWGG (W is A or T) are methylated by the Dcm enzyme. These sites...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00371-18
更新日期:2018-11-26 00:00:00
abstract::Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.344-353.1975
更新日期:1975-01-01 00:00:00
abstract::Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.3.700-708.1999
更新日期:1999-02-01 00:00:00
abstract::Beggs, William H. (University of Minnesota, Minneapolis), and Palmer Rogers. Galactose repression of beta-galactosidase induction in Escherichia coli. J. Bacteriol. 91:1869-1874. 1966.-Galactose repression of beta-galactosidase induction in Escherichia coli was investigated to determine whether the galactose molecule ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1869-1874.1966
更新日期:1966-05-01 00:00:00
abstract::The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.507-509.1982
更新日期:1982-07-01 00:00:00
abstract::Double-stranded, 1.9-kilobase-pair (kbp) DNA molecules were found in 18 strains representing three pathogenic races of Fusarium oxysporum f. sp. conglutinans. The DNA element (pFOXC1) from a race 1 strain and the DNA element (pFOXC2) from a race 2 strain were shown by restriction endonuclease mapping to be linear. pFO...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.587-593.1986
更新日期:1986-08-01 00:00:00
abstract::Isolated sheaths of Leptothrix discophora SP-6 (ATCC 51168) were tested for susceptibility to degradation by a variety of chemical denaturants and lytic enzymes and found to be resistant to many reagents and enzyme treatments. However, disulfide bond-reducing agents such as dithiothreitol (DTT), beta-mercaptoethanol, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.7819-7827.1993
更新日期:1993-12-01 00:00:00
abstract::Copper (Cu) is a required micronutrient, but it is highly toxic at high concentrations. Therefore, the levels of Cu must be tightly regulated in all living cells. The phagosome of Mycobacterium tuberculosis has been shown to have variable levels of Cu. Previously, we showed that M. tuberculosis contains a copper-sensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01847-07
更新日期:2008-04-01 00:00:00
abstract::Listeria monocytogenes is a model facultative intracellular pathogen. Tight regulation of virulence proteins is essential for a successful infection, and the gene encoding the annotated thioredoxin YjbH was identified in two forward genetic screens as required for virulence factor production. Accordingly, an L. monocy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00099-20
更新日期:2020-05-27 00:00:00
abstract::The salmonellae are a diverse group of bacteria within the family Enterobacteriaceae that includes two species, Salmonella enterica and Salmonella bongori. In order to characterize the phylogenetic relationships of the species and subspecies of Salmonella, we analyzed four housekeeping genes, gapA, phoP, mdh and recA,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01552-07
更新日期:2008-11-01 00:00:00
abstract::Survivors of nitrosoguanidine-treated cultures of a colicinogenic strain of Salmonella typhimurium were tested for spontaneous production of colicin E1. Of about 1,000 colonies tested, 13 produced no (or very narrow) colicin zones. Four of these isolates proved to be more sensitive to ultraviolet (UV) light, X rays, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.345-350.1970
更新日期:1970-10-01 00:00:00
abstract::Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.21.5966-5970.2002
更新日期:2002-11-01 00:00:00