Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase.

abstract：:We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124,...

journal_title：Journal of bacteriology

authors： Addinall SG,Small E,Whitaker D,Sturrock S,Donachie WD,Khattar MM

更新日期：2005-01-01 00:00:00

abstract：:Chlamydia trachomatis is a human bacterial pathogen that multiplies only within an intracellular membrane-bound vacuole, the inclusion. C. trachomatis includes ocular and urogenital strains, usually causing infections restricted to epithelial cells of the conjunctiva and genital mucosa, respectively, and lymphogranulo...

journal_title：Journal of bacteriology

authors： Almeida F,Borges V,Ferreira R,Borrego MJ,Gomes JP,Mota LJ

更新日期：2012-12-01 00:00:00

abstract：:We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...

journal_title：Journal of bacteriology

authors： Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

更新日期：1988-08-01 00:00:00

abstract：:Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...

journal_title：Journal of bacteriology

authors： Qi Y,Kobayashi Y,Hulett FM

更新日期：1997-04-01 00:00:00

abstract：:The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted ...

journal_title：Journal of bacteriology

authors： Kitano K,Tuomanen E,Tomasz A

更新日期：1986-09-01 00:00:00

abstract：:The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to prod...

journal_title：Journal of bacteriology

authors： Heffron JD,Sherry N,Popham DL

更新日期：2011-01-01 00:00:00

abstract：:Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...

journal_title：Journal of bacteriology

authors： Noriea NF,Clark TR,Mead D,Hackstadt T

更新日期：2017-02-28 00:00:00

abstract：:Flavobacterium johnsoniae cells move rapidly over surfaces by gliding motility. Gliding results from the movement of adhesins such as SprB and RemA along the cell surface. These adhesins are delivered to the cell surface by a Bacteroidetes-specific secretion system referred to as the type IX secretion system (T9SS). G...

journal_title：Journal of bacteriology

authors： Shrivastava A,Johnston JJ,van Baaren JM,McBride MJ

更新日期：2013-07-01 00:00:00

abstract：:The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...

journal_title：Journal of bacteriology

authors： Hennig S,Ziebuhr W

更新日期：2008-02-01 00:00:00

abstract：:Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...

journal_title：Journal of bacteriology

authors： Pérez-Pantoja D,Ledger T,Pieper DH,González B

更新日期：2003-03-01 00:00:00

abstract：:The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...

journal_title：Journal of bacteriology

authors： Aygun-Sunar S,Bilaloglu R,Goldfine H,Daldal F

更新日期：2007-12-01 00:00:00

abstract：:Bacterial cells sense the extracellular environment and adapt to that environment by activating gene regulation circuits, often by means of signaling molecules. The Serratia marcescens hemophore is a signaling molecule that acts as an extracellular heme-scavenging protein. The heme-loaded hemophore interacts with its ...

journal_title：Journal of bacteriology

authors： Cwerman H,Wandersman C,Biville F

更新日期：2006-05-01 00:00:00

abstract：:The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. Muta...

journal_title：Journal of bacteriology

authors： Stanley NR,Findlay K,Berks BC,Palmer T

更新日期：2001-01-01 00:00:00

abstract：:Two isoleucine analogues and two leucine analogues were examined for their ability to replace the natural amino acid preventing the accumulation of threonine deaminase-forming potential. The procedure used to study repression by the analogues distinguishes between true repression and the formation of inactive enzyme b...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1973-11-01 00:00:00

abstract：:High-resolution electron microscopy of polarly flagellated bacteria revealed that their flagella originate at a circular, differentiated portion of the cytoplasmic membrane approximately 25 nm in diameter. The flagella also have discs attaching them to the cell wall. These attachment discs are extremely resistant to l...

journal_title：Journal of bacteriology

authors： Vaituzis Z,Doetsch RN

更新日期：1969-10-01 00:00:00

abstract：:A spontaneously arising regulatory mutant of the gluconate system in Escherichia coli was isolated. This mutant became constitutive, probably in one step, for gluconate high-affinity transport, gluconokinase, and gluconate-6-P dehydrase. The mutation involved (gntR18) is cotransducible with asd. Pseudorevertants, deri...

journal_title：Journal of bacteriology

authors： Zwaig N,Nagel de Zwaig R,Istúriz T,Wecksler M

更新日期：1973-05-01 00:00:00

abstract：:Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...

journal_title：Journal of bacteriology

authors： Rehm BH,Boheim G,Tommassen J,Winkler UK

更新日期：1994-09-01 00:00:00

abstract：:Salmonella enterica can utilize fructose-asparagine (F-Asn) as a source of carbon and nitrogen. This capability has been attributed to five genes in the fra locus. Previously, we determined that mutations in fraB (deglycase), fraD (kinase), or fraA (transporter) eliminated the ability of Salmonella to grow on F-Asn, w...

journal_title：Journal of bacteriology

authors： Sabag-Daigle A,Sengupta A,Blunk HM,Biswas PK,Cron MC,Bogard AJ,Behrman EJ,Gopalan V,Ahmer BMM

更新日期：2017-10-17 00:00:00

abstract：:A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...

journal_title：Journal of bacteriology

authors： Tomoyasu T,Arsène F,Ogura T,Bukau B

更新日期：2001-10-01 00:00:00

abstract：:We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...

journal_title：Journal of bacteriology

authors： Simonsen CC,Chen EY,Levinson AD

更新日期：1983-09-01 00:00:00

abstract：:The type III secretion system (T3SS) of Pseudomonas aeruginosa is an important virulence factor. The T3SS of P. aeruginosa can be induced by a low calcium signal or upon direct contact with the host cells. The exact pathway of signal sensing and T3SS activation is not clear. By screening a transposon insertion mutant ...

journal_title：Journal of bacteriology

authors： Wu W,Badrane H,Arora S,Baker HV,Jin S

更新日期：2004-11-01 00:00:00

abstract：:Antiserum was made against a single 30S protein, 30S-7. The amount of complement fixed by total 30S protein in the presence of this antiserum indicated that protein 30S-7 was the major antigen in the mixture of proteins. Each of the 30S ribosomal proteins was tested for cross-reactivity with anti-30S-7. This was done ...

journal_title：Journal of bacteriology

authors： Strnad BC,Sypherd PS

更新日期：1971-11-01 00:00:00

abstract：:Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine a...

journal_title：Journal of bacteriology

authors： PIZER LI

更新日期：1965-04-01 00:00:00

abstract：:Maleylacetate reductase (EC 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. Several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precu...

journal_title：Journal of bacteriology

authors： Kaschabek SR,Reineke W

更新日期：1995-01-01 00:00:00

abstract：:Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...

journal_title：Journal of bacteriology

authors： Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

更新日期：1998-01-01 00:00:00

abstract：:The response of Neurospora to changes in the availibility of exogenous arginine was investigated. Upon addition of arginine to the growth medium, catabolism is initiated within minutes. This occurs prior to expansion of the arginine pool or augmentation of catabolic enzyme levels. (Basal levels are approximately 25% o...

journal_title：Journal of bacteriology

authors： Weiss RL,Davis RH

更新日期：1977-02-01 00:00:00

abstract：:The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered w...

journal_title：Journal of bacteriology

authors： Organick AB,Lutsky II

更新日期：1968-07-01 00:00:00

abstract：:lisRK encodes a two-component regulatory system in the food pathogen Listeria monocytogenes LO28. Following identification of the operon in an acid-tolerant Tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol c...

journal_title：Journal of bacteriology

authors： Cotter PD,Emerson N,Gahan CG,Hill C

更新日期：1999-11-01 00:00:00

abstract：:Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

journal_title：Journal of bacteriology

authors： HOWARD DH

更新日期：1964-01-01 00:00:00

abstract：:Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...

journal_title：Journal of bacteriology

authors： Troiano AJ Jr,Zhang J,Cowan AE,Yu J,Setlow P

更新日期：2015-01-01 00:00:00