Regulatory mutations affecting the gluconate system in Escherichia coli.

abstract：:The maltose regulon consists of three operons controlled by a positive regulatory gene, malT. Deletions of the gene crp were introduced into strains which carried a malT-lacZ hybrid gene. From the observed reduction in beta-galactosidase activity it was concluded that the expression of malT-lacZ, and therefore of malT...

journal_title：Journal of bacteriology

authors： Chapon C

更新日期：1982-05-01 00:00:00

abstract：:PacI and SpeI restriction maps were obtained for the two chromosomes of each of the six species of the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, and B. neotomae. Three complementary techniques were used: hybridization with the two replicons as probes, cross-hybridization of restriction fra...

journal_title：Journal of bacteriology

authors： Michaux-Charachon S,Bourg G,Jumas-Bilak E,Guigue-Talet P,Allardet-Servent A,O'Callaghan D,Ramuz M

更新日期：1997-05-01 00:00:00

abstract：:In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...

journal_title：Journal of bacteriology

authors： Simon HM,Homer MJ,Roberts GP

更新日期：1996-05-01 00:00:00

abstract：:The antibiotic peptide nisin is the first known lantibiotic that uses a docking molecule within the bacterial cytoplasmic membrane for pore formation. Through specific interaction with the cell wall precursor lipid II, nisin forms defined pores which are stable for seconds and have pore diameters of 2 to 2.5 nm. ...

journal_title：Journal of bacteriology

authors： Wiedemann I,Benz R,Sahl HG

更新日期：2004-05-01 00:00:00

abstract：:In Escherichia coli, the following genes are involved in motility and chemotaxis. The H gene is the structural gene for flagellin. Mutation in the mot gene results in paralysis of the flagella, and mutation in the fla genes leads to an absence of flagella. The cheA, cheB, and cheC genes are required for chemotaxis. Th...

journal_title：Journal of bacteriology

authors： Armstrong JB,Adler J

更新日期：1969-01-01 00:00:00

abstract：:When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine...

journal_title：Journal of bacteriology

authors： Rana RS,Halvorson HO

更新日期：1972-02-01 00:00:00

abstract：:In previous work with membranes of Bacillus subtilis, the succinate dehydrogenase complex was isolated by immunoprecipitation of Triton X-100-solubilized membranes. The complex included a polypeptide with an apparent molecular weight of 19,000, probably attributable to apocytochrome. This paper reports the further cha...

journal_title：Journal of bacteriology

authors： Hederstedt L

更新日期：1980-12-01 00:00:00

abstract：:Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...

journal_title：Journal of bacteriology

authors： Rehm BH,Boheim G,Tommassen J,Winkler UK

更新日期：1994-09-01 00:00:00

abstract：:Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in in UvrB strains containing both plasmid pNP5 and...

journal_title：Journal of bacteriology

authors： Pannekoek H,Noordermeer I,van de Putte P

更新日期：1979-07-01 00:00:00

abstract：:Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved...

journal_title：Journal of bacteriology

authors： Nelson SS,Glocka PP,Agarwal S,Grimm DP,McBride MJ

更新日期：2007-10-01 00:00:00

abstract：:Salmonella enterica degrades 1,2-propanediol (1,2-PD) in a coenzyme B12 (adenosylcobalamin, AdoCbl)-dependent fashion. Salmonella obtains AdoCbl by assimilation of complex precursors, such as vitamin B12 and hydroxocobalamin. Assimilation of these compounds requires reduction of their central cobalt atom from Co3+ to ...

journal_title：Journal of bacteriology

authors： Cheng S,Bobik TA

更新日期：2010-10-01 00:00:00

abstract：:The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...

journal_title：Journal of bacteriology

authors： Ying CW,Ordal GW

更新日期：1989-03-01 00:00:00

abstract：:A transformable strain of Bacillus subtilis 6160, a derivative of B. subtilis 168, produces three kinds of casein hydrolytic enzymes (alkaline protease, neutral protease, and esterase) in a culture medium. B. natto IAM 1212 produces 15 to 20 times as much total proteolytic activity as does B. subtilis. Extracellular p...

journal_title：Journal of bacteriology

authors： Uehara H,Yoneda Y,Yamane K,Maruo B

更新日期：1974-07-01 00:00:00

abstract：:Small subunit rDNA sequences were determined for 20 species of the genera Acetogenium, Clostridium, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobium, and Thermobacteroides, 3 non-validly described species, and 5 isolates of anaerobic thermophilic bacteria, providing a basis for a phylogenetic analysis of th...

journal_title：Journal of bacteriology

authors： Rainey FA,Ward NL,Morgan HW,Toalster R,Stackebrandt E

更新日期：1993-08-01 00:00:00

abstract：:For the ornithine fermentation pathway, described more than 70 years ago, genetic and biochemical information are still incomplete. We present here the experimental identification of the last four missing genes of this metabolic pathway. They encode L-ornithine racemase, (2R,4S)-2,4-diaminopentanoate dehydrogenase, an...

journal_title：Journal of bacteriology

authors： Fonknechten N,Perret A,Perchat N,Tricot S,Lechaplais C,Vallenet D,Vergne C,Zaparucha A,Le Paslier D,Weissenbach J,Salanoubat M

更新日期：2009-05-01 00:00:00

abstract：:We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...

journal_title：Journal of bacteriology

authors： Winans SC,Kerstetter RA,Nester EW

更新日期：1988-09-01 00:00:00

abstract：:Escherichia coli K-12 deficient in galactose-1-phosphate uridyl transferase is capable of converting significant amounts of d-[1-(14)C]galactose to (14)CO(2), whereas strains deficient in other enzymes of the Leloir pathway cannot do so. ...

journal_title：Journal of bacteriology

authors： LaPolla RJ,Geier MR,Friedman TB,Merril CR

更新日期：1975-10-01 00:00:00

abstract：:We report here the identification of a new lipoprotein, NlpI, in Escherichia coli K-12. The NlpI structural gene (nlpI) is located between the genes pnp (polynucleotide phosphorylase) and deaD (RNA helicase) at 71 min on the E. coli chromosome. The nlpI gene encodes a putative polypeptide of approximately 34 kDa, and ...

journal_title：Journal of bacteriology

authors： Ohara M,Wu HC,Sankaran K,Rick PD

更新日期：1999-07-01 00:00:00

abstract：:When cells of two auxotrophic mutants of Sulfolobus acidocaldarius are mixed and incubated on solid medium, they form stable genetic recombinants which can be selected, enumerated, and characterized. Any of a variety of auxotrophic markers can recombine with each other, and the phenomenon has been observed at temperat...

journal_title：Journal of bacteriology

authors： Grogan DW

更新日期：1996-06-01 00:00:00

abstract：:Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...

journal_title：Journal of bacteriology

authors： Norlander L,Davies JK,Hagblom P,Normark S

更新日期：1981-02-01 00:00:00

abstract：:A number of laboratories are now engaged in the genetic analysis of Candida albicans. This diploid yeast, the major fungal pathogen of humans, is imperfect. Parasexual techniques have been devised for complementation and recombination analysis in this organism. This paper attempts to address the question of the extent...

journal_title：Journal of bacteriology

authors： Poulter R,Hanrahan V

更新日期：1983-11-01 00:00:00

abstract：:The potent tumor-promoting agent croton oil, which has been shown previously to be strongly mitogenic in mammalian cells, stimulates cell division in snake mutants of the blue-green bacterium Agmenellum quadruplicatum. ...

journal_title：Journal of bacteriology

authors： Ingram LO,Fisher WD

更新日期：1973-05-01 00:00:00

abstract：:Formylglutamate amidohydrolase (FGase) catalyzes the terminal reaction in the five-step pathway for histidine utilization in Pseudomonas putida. By this action, N-formyl-L-glutamate (FG) is hydrolyzed to produce L-glutamate plus formate. Urocanate, the first product in the pathway, induced all five enzymes, but FG was...

journal_title：Journal of bacteriology

authors： Hu L,Mulfinger LM,Phillips AT

更新日期：1987-10-01 00:00:00

abstract：:A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...

journal_title：Journal of bacteriology

authors： Dietzler DN,Leckie MP,Crimmins DL,Ungar JM,Sternheim WL

更新日期：1976-10-01 00:00:00

abstract：:Ureolytic clinical isolates of Providencia stuartii, Salmonella spp., and some Escherichia coli strains contain large urease-encoding plasmids. Expression of urease activity from these isolates is induced at least 20-fold by urea. In order to facilitate studies on the regulatory mechanism controlling this urea-inducib...

journal_title：Journal of bacteriology

authors： D'Orazio SE,Collins CM

更新日期：1993-06-01 00:00:00

abstract：:Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...

journal_title：Journal of bacteriology

authors： Merlo DJ,Nester EW

更新日期：1977-01-01 00:00:00

abstract：:Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...

journal_title：Journal of bacteriology

authors： Xu D,Yang C,Kaplan HB

更新日期：1998-12-01 00:00:00

abstract：:Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...

journal_title：Journal of bacteriology

authors： Brambl R

更新日期：1975-06-01 00:00:00

abstract：:We have presented experimental evidence to support the model that gliding motility of Myxococcus xanthus is driven by surface tension. (i) Motility is inhibited by the addition of sufficient exogenous, nontoxic surfactants to swamp out the cells' own surfactant gradient. (ii) M. xanthus does not move polystyrene latex...

journal_title：Journal of bacteriology

authors： Dworkin M,Keller KH,Weisberg D

更新日期：1983-09-01 00:00:00

abstract：:Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...

journal_title：Journal of bacteriology

authors： Chaillou S,Lokman BC,Leer RJ,Posthuma C,Postma PW,Pouwels PH

更新日期：1998-05-01 00:00:00