Abstract:
:For the ornithine fermentation pathway, described more than 70 years ago, genetic and biochemical information are still incomplete. We present here the experimental identification of the last four missing genes of this metabolic pathway. They encode L-ornithine racemase, (2R,4S)-2,4-diaminopentanoate dehydrogenase, and the two subunits of 2-amino-4-ketopentanoate thiolase. While described only for the Clostridiaceae to date, this pathway is shown to be more widespread.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Fonknechten N,Perret A,Perchat N,Tricot S,Lechaplais C,Vallenet D,Vergne C,Zaparucha A,Le Paslier D,Weissenbach J,Salanoubat Mdoi
10.1128/JB.01777-08subject
Has Abstractpub_date
2009-05-01 00:00:00pages
3162-7issue
9eissn
0021-9193issn
1098-5530pii
JB.01777-08journal_volume
191pub_type
杂志文章abstract::Escherichia coli B dependence on CO2 for growth was demonstrated. At suboptimal CO2 concentrations the rate of growth was controlled by CO2 concentration. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.1162-1164.1978
更新日期:1978-09-01 00:00:00
abstract::Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.6.1623-1630.1996
更新日期:1996-03-01 00:00:00
abstract::Mycoplasma iowae is associated mainly with reduced hatchability in turkeys and is well known for the unusual ability of phenotypic variation in the Mycoplasma surface components as well as a relative resistance to heat, bile salts, and many antimicrobials. A subset of unique genes and a gene cluster responsible for th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06297-11
更新日期:2012-01-01 00:00:00
abstract::Phytoplasmas are plant-pathogenic bacteria that cause numerous diseases. This study shows a strong positive selection on the phytoplasma antigenic membrane protein (Amp). The ratio of nonsynonymous to synonymous substitutions was >1 with all the methods we tested. The clear positive selections imply an important biolo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.9.3424-3428.2006
更新日期:2006-05-01 00:00:00
abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05245-11
更新日期:2011-08-01 00:00:00
abstract::Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.674-681.1972
更新日期:1972-09-01 00:00:00
abstract::High-affinity iron acquisition in Bacillus subtilis is mediated via the bacillibactin catechole siderophore pathway. Three of the four essential pathway steps, bacillibactin synthesis, Fe-bacillibactin uptake, and Fe-bacillibactin hydrolysis have been characterized previously. The functional and regulatory components ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00464-08
更新日期:2008-08-01 00:00:00
abstract::Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, have been cloned and sequenced, and they encode principal sigma factors of the RNA polymerase. The deduced amino acid sequences of SigA and SigB showed very high similarities to th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.2.550-553.1996
更新日期:1996-01-01 00:00:00
abstract::Legionella pneumophila expresses two peroxide-scavenging alkyl hydroperoxide reductase systems (AhpC1 and AhpC2D) that are expressed differentially during the bacterial growth cycle. Functional loss of the postexponentially expressed AhpC1 system is compensated for by increased expression of the exponentially expresse...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00141-08
更新日期:2008-05-01 00:00:00
abstract::Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.4056-4063.1990
更新日期:1990-07-01 00:00:00
abstract::The physiological effects of incubation at nonpermissive temperatures of Escherichia coli mutants that carry a temperature-sensitive dnaZ allele [dnaZ(Ts)2016] were examined. The temperature at which the dnaZ(Ts) protein becomes inactivated in vivo was investigated by measurements of deoxyribonucleic acid (DNA) synthe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.1.151-158.1977
更新日期:1977-10-01 00:00:00
abstract::A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynami...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.21.6056-6059.2002
更新日期:2002-11-01 00:00:00
abstract::The effects of variations in growth conditions on the penicillin response of Streptococcus faecium ATCC 9790 were studied. Changes in the growth temperature and medium composition were found to cause striking changes in the bacterial generation time, cellular penicillin sensitivity (minimum inhibitory concentration), ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.2.916-923.1983
更新日期:1983-05-01 00:00:00
abstract::Aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type II secretory pathway or secreton. ExeA is an essential component of this system and is necessary for the localization and/or multimerization of the secretin ExeD. ExeA contains two sequence motifs characteris...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.18.6370-6378.2005
更新日期:2005-09-01 00:00:00
abstract::Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.22.7136-7141.1991
更新日期:1991-11-01 00:00:00
abstract::In this report, we show that biofilm formation by Streptococcus pneumoniae serotype 19 gives rise to variants (the small mucoid variant [SMV] and the acapsular small-colony variant [SCV]) differing in capsule production, attachment, and biofilm formation compared to wild-type strains. All biofilm-derived variants harb...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00707-08
更新日期:2008-10-01 00:00:00
abstract::The mevalonate diphosphate decarboxylase is an enzyme which converts mevalonate diphosphate to isopentenyl diphosphate, the building block of isoprenoids. We used the Saccharomyces cerevisiae temperature-sensitive mutant defective for mevalonate diphosphate decarboxylase previously described (C. Chambon, V. Ladeveve, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4664-4670.1997
更新日期:1997-08-01 00:00:00
abstract::A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3898-3902.2005
更新日期:2005-06-01 00:00:00
abstract::Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.14.4012-4021.2000
更新日期:2000-07-01 00:00:00
abstract::Enterococcus faecalis is associated with a high proportion of nosocomial infections; however, little is known of the ability of this organism to proliferate in vivo. The ability of RNase B, a model glycoprotein with a single N-glycosylation site occupied by a family of high-mannose-type glycans (Man(5)- to Man(9)-GlcN...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.4.882-890.2000
更新日期:2000-02-01 00:00:00
abstract::Arginine deiminase activity was induced during the vegetative growth of Clostridium sporogenes. The enzyme was sensitive to catabolite repression. The other enzymes of the arginine dihydrolase pathway, namely, ornithine carbamoyl-transferase and carbamate kinase, did not show such variation. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.693-695.1977
更新日期:1977-08-01 00:00:00
abstract::Different preparations of the methylreductase were tested in a simplified methylcoenzyme M methylreductase assay with artificial electron donors under a nitrogen atmosphere. ATP and Mg2+ stimulated the reaction. Tris(2,2'-bipyridine)ruthenium (II), chromous chloride, chromous acetate, titanium III citrate, 2,8-diamino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2711-2715.1988
更新日期:1988-06-01 00:00:00
abstract::It was previously shown that the Rhodobacter capsulatus NtrC enhancer-binding protein activates the R. capsulatus housekeeping RNA polymerase but not the Escherichia coli RNA polymerase at the nifA1 promoter. We have tested the hypothesis that this activity is due to the high G+C content of the -10 sequence. A compara...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.3.740-749.2004
更新日期:2004-02-01 00:00:00
abstract::Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.479-489.1979
更新日期:1979-11-01 00:00:00
abstract::During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.5834-5839.2001
更新日期:2001-10-01 00:00:00
abstract::We previously described 14 rare spontaneous morphological mutants of Candida albicans that were associated with chromosomal aberrations (E. P. Rustchenko-Bulgac, F. Sherman, and J. B. Hicks, J. Bacteriol. 172:1276-1283, 1990). Improved conditions for separation of chromosomes, as well as hybridization probes, were use...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.20.6586-6596.1991
更新日期:1991-10-01 00:00:00
abstract::The 6th American Society for Microbiology Conference on Cell-Cell Communication in Bacteria convened from 16 to 19 October 2017 in Athens, GA. In this minireview, we highlight some of the research presented at that meeting that addresses central questions emerging in the field, including the following questions. How a...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00291-18
更新日期:2018-06-25 00:00:00
abstract::Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1332-1336.1985
更新日期:1985-12-01 00:00:00
abstract::Deletion of the citC gene, coding for isocitrate dehydrogenase, arrests sporulation of Bacillus subtilis at stage I after bipolar localization of the cell division protein FtsZ but before formation of the asymmetric septum. A spontaneous extragenic suppressor mutation that overcame the stage I block was found to map w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.11.3392-3401.1999
更新日期:1999-06-01 00:00:00
abstract::A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.440-446.1979
更新日期:1979-01-01 00:00:00