Porphyrin binding by the surface array virulence protein of Aeromonas salmonicida.

Abstract:

:Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells by a kinetically distinct mechanism (Kd, 0.25 microM) which was absent in A- isogenic strains. Purified A-layer protein ("A protein") protein A also bound Congo red with similar affinity (Kd, 0.40 microM). Congo red binding was structurally specific; it was not influenced by a wide variety of compounds including amino acids and nucleotides and only weakly inhibited by structurally similar dyes. However, protoporphyrin IX and hemin were strong competitive inhibitors of Congo red binding. Protoporphyrin and hemin were bound only by A+ strains (KdS of 0.41 and 0.63 microM, respectively). Furthermore, binding of these porphyrins was strongly inhibited by Congo red but weakly inhibited by hematoporphyrin. Purified A protein also bound protoporphyrin IX and hemin with affinities similar to those of A+ cells (KdS of 0.94 and 0.41 microM, respectively.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kay WW,Phipps BM,Ishiguro EE,Trust TJ

doi

10.1128/JB.164.3.1332-1336.1985

subject

Has Abstract

pub_date

1985-12-01 00:00:00

pages

1332-6

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

164

pub_type

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