Abstract:
:Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells by a kinetically distinct mechanism (Kd, 0.25 microM) which was absent in A- isogenic strains. Purified A-layer protein ("A protein") protein A also bound Congo red with similar affinity (Kd, 0.40 microM). Congo red binding was structurally specific; it was not influenced by a wide variety of compounds including amino acids and nucleotides and only weakly inhibited by structurally similar dyes. However, protoporphyrin IX and hemin were strong competitive inhibitors of Congo red binding. Protoporphyrin and hemin were bound only by A+ strains (KdS of 0.41 and 0.63 microM, respectively). Furthermore, binding of these porphyrins was strongly inhibited by Congo red but weakly inhibited by hematoporphyrin. Purified A protein also bound protoporphyrin IX and hemin with affinities similar to those of A+ cells (KdS of 0.94 and 0.41 microM, respectively.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kay WW,Phipps BM,Ishiguro EE,Trust TJdoi
10.1128/JB.164.3.1332-1336.1985subject
Has Abstractpub_date
1985-12-01 00:00:00pages
1332-6issue
3eissn
0021-9193issn
1098-5530journal_volume
164pub_type
杂志文章abstract::LéJohn, Herbert B. (Purdue University, Lafayette, Ind.), and James S. Lovett. Ribonucleic acid and protein synthesis in Rhizophlyctis rosea zoospores. J. Bacteriol. 91:709-717. 1966.-The uniflagellate zoospores of Rhizophlyctis rosea display active motility and a high endogenous respiratory metabolism, but neither gro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.709-717.1966
更新日期:1966-02-01 00:00:00
abstract::Arginine deiminase activity was induced during the vegetative growth of Clostridium sporogenes. The enzyme was sensitive to catabolite repression. The other enzymes of the arginine dihydrolase pathway, namely, ornithine carbamoyl-transferase and carbamate kinase, did not show such variation. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.693-695.1977
更新日期:1977-08-01 00:00:00
abstract::Our whole-genome microarray studies of Neisseria meningitidis MC58 previously identified a set of 153 genes whose transcription was activated during growth in iron. In this study, Fur-mediated regulation of the iron-activated nspA gene was confirmed, whereas iron-activated regulation of the secY gene was demonstrated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01638-06
更新日期:2007-01-01 00:00:00
abstract::Pathogenic Yersinia species employ a type III secretion system (TTSS) to target antihost factors, Yop proteins, into eukaryotic cells. The secretion machinery is constituted of ca. 20 Ysc proteins, nine of which show significant homology to components of the flagellar TTSS. A key event in flagellar assembly is the swi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.16.4500-4509.2002
更新日期:2002-08-01 00:00:00
abstract::We investigated the effect of time, temperature, and the presence of sodium chloride, nitrates, and nitrites in the medium on the growth and production of enterotoxin B by Staphylococcus aureus. Assays by the double gel-diffusion method showed that maximal enterotoxin B production occurs at the beginning of the statio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.4.1207-1211.1968
更新日期:1968-04-01 00:00:00
abstract::The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00622-08
更新日期:2008-09-01 00:00:00
abstract::The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.20.5885-5892.2000
更新日期:2000-10-01 00:00:00
abstract::Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.545-555.1976
更新日期:1976-02-01 00:00:00
abstract::Sequences essential to the chloramphenicol-inducible expression of cat-86, a chloramphenicol acetyltransferase gene, reside in a 144-base pair (bp) regulatory region that intervenes between the cat-86 coding sequence and its promoter. A key regulatory element within the 144-bp segment consists of a pair of inverted-re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.696-703.1985
更新日期:1985-11-01 00:00:00
abstract::Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies reveal that the plasmids coding for the production of heat stable and heat labile enteroxtoxins of Escherichia coli, regardless of their origin, have a majority of their polynucleotide sequences in common, but are not related in any significant way to t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.234-238.1975
更新日期:1975-01-01 00:00:00
abstract::Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00592-09
更新日期:2009-10-01 00:00:00
abstract::A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.2.476-482.2001
更新日期:2001-01-01 00:00:00
abstract::The content and distribution of autolysin were measured in temperature-sensitive morphological mutants of Bacillus subtilis. Strains RUB1000 and RUB1012 grew as rods at 30 C. At 45 C the mutants contained disproportionately less teichoic acid than peptidoglycan and grew as irregular spheres. The amount of enzyme that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.166-173.1976
更新日期:1976-01-01 00:00:00
abstract::Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.301-308.2001
更新日期:2001-01-01 00:00:00
abstract::We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.88) of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5567-5571.1989
更新日期:1989-10-01 00:00:00
abstract::Sequences of the 16S ribosomal DNA (rDNA) from psychrotolerant and mesophilic strains of the Bacillus cereus group revealed signatures which were specific for these two thermal groups of bacteria. Further analysis of the genomic DNA from a wide range of food and soil isolates showed that B. cereus group strains have b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2624-2630.1999
更新日期:1999-04-01 00:00:00
abstract::The position of the termination codon in lacZX90 was determined by isolation of a lac+ revertant. Lysine was found to replace tyrosine at position 1,012 of beta-galactosidase, indicating that X90 protein lacked the carboxyl-terminal 10 residues. A heat- and urea-sensitive hybrid enzyme was formed in vivo when supC, wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.694-697.1981
更新日期:1981-08-01 00:00:00
abstract::Sinorhizobium meliloti, the nitrogen-fixing symbiont of alfalfa, has the ability to catabolize myo-, scyllo-, and D-chiro-inositol. Functional inositol catabolism (iol) genes are required for growth on these inositol isomers, and they play a role during plant-bacterium interactions. The inositol catabolism genes compr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05371-11
更新日期:2011-10-01 00:00:00
abstract::Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens, such as Geobacter sulfurreducens and Shewanella oneidensis, are Gram negative. More recently, it has been reported that Gram-positive bacteria, such as L...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00029-20
更新日期:2020-03-11 00:00:00
abstract::Riemerella anatipestifer is a Gram-negative, rod-shaped bacterium associated with epizootic infections in poultry. R. anatipestifer strain RA-YM, belonging to the serotype 1 prevalent in China, is a clinically isolated strain with high-level virulence. Here, we report the first genome sequence of this species. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01445-10
更新日期:2011-03-01 00:00:00
abstract::Extracellular fibrils are involved in cell cohesion and cell development in Myxococcus xanthus. One group of social motility mutants, Dsp, is unable to produce extracellular fibrils; these mutants also lose the abilities to cohere and to develop. Extracellular fibrils isolated from vegetative wild-type cells and added...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7190-7196.1994
更新日期:1994-12-01 00:00:00
abstract::In Escherichia coli, nine essential cell division proteins are known to localize to the division septum. FtsL is a 13-kDa bitopic membrane protein with a short cytoplasmic N-terminal domain, a membrane-spanning segment, and a periplasmic domain that has a repeated heptad motif characteristic of leucine zippers. Here, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.116-129.2000
更新日期:2000-01-01 00:00:00
abstract::Lockart, Royce Z., Jr. (The University of Texas, Austin) and Barbara Horn. Interaction of an interferon with L cells. J. Bacteriol. 85:996-1002. 1963.-Data were presented on the effect of time of exposure and concentration of an interferon in provoking viral inhibition in L cells. Populations of L cells which made red...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.996-1002.1963
更新日期:1963-05-01 00:00:00
abstract::From a lysogen with lambda integrated in the leu operon, specialized transducing phages that carry the cell division, murein biosynthesis, and envelope permeability genes located about 0.5 min to the right of leu were isolated. These phages were used to identify the previously undiscovered cell division gene sep. A ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.91-100.1978
更新日期:1978-01-01 00:00:00
abstract::The morphology of nucleoids and mesosomes of Bacillus subtilis in stationary-and lag-phase cultures was studied by making three-dimensional cell reconstructions in plastic of electron micrographs of serial sections. In cells from stationary cultures, the dormant nucleoids are frequently, but not always, spherical and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.1189-1199.1975
更新日期:1975-03-01 00:00:00
abstract::Lectin activity in a host-mycoparasite relationship was demonstrated with Rhizoctonia solani and Trichoderma harzianum. Attachment of O but not A and B erythrocytes to hyphae occurred on R. solani but not on its mycoparasite. This phenomenon, which was Ca2+ and Mn2+ dependent, was prevented by galactose, present in T....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1431-1435.1983
更新日期:1983-06-01 00:00:00
abstract::Transcription of the archaeal trkA gene homolog in Methanosarcina mazei S-6 was studied at the optimal growth temperature of 37 degrees C and after heat shock at 45 degrees C. Northern (RNA) blotting results (transcript size) and data from primer extension experiments to map the transcription initiation site indicate ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6077-6082.1995
更新日期:1995-11-01 00:00:00
abstract::In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01063-08
更新日期:2009-01-01 00:00:00
abstract::PsaA, the subunit of the fimbria originally referred to as the "pH 6 antigen," is required for full virulence of Yersinia pestis during bubonic plague. The expression of psaA is dependent upon specific environmental signals, and while the signals (high temperature and acidic pH) are defined, the mechanisms underlying ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00217-19
更新日期:2019-07-24 00:00:00
abstract::To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.24.7449-7456.1999
更新日期:1999-12-01 00:00:00