Abstract:
:Deletion of the citC gene, coding for isocitrate dehydrogenase, arrests sporulation of Bacillus subtilis at stage I after bipolar localization of the cell division protein FtsZ but before formation of the asymmetric septum. A spontaneous extragenic suppressor mutation that overcame the stage I block was found to map within the spoVG gene. The suppressing mutation and other spoVG loss-of-function mutations enabled citC mutant cells to form asymmetric septa and to activate the forespore-specific sigma factor sigmaF. However, little induction of mother cell-specific, sigmaE-dependent sporulation genes was observed in a citC spoVG double mutant, indicating that there is an additional defect(s) in compartmentalized gene expression in the citC mutant. These other defects could be partially overcome by reducing the synthesis of citrate, by buffering the medium, or by adding excess MnCl2. Overexpression of the spoVG gene in wild-type cells significantly delayed sigmaF activation. Increased expression and stability of SpoVG in citC mutant cells may contribute to the citC mutant phenotype. Inactivation of the spoVG gene caused a population of otherwise wild-type cells to produce a small number of minicells during growth and caused sporulating cells to complete asymmetric septation more rapidly than normal. Unlike the case for inactivation of the cell division inhibitor gene minD, many of these minicells contained DNA and appeared only when the primary sporulation signal transduction pathway, the Spo0A phosphorelay, was active. These results suggest that SpoVG interferes with or is a negative regulator of the pathway leading to asymmetric septation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Matsuno K,Sonenshein ALdoi
10.1128/JB.181.11.3392-3401.1999keywords:
subject
Has Abstractpub_date
1999-06-01 00:00:00pages
3392-401issue
11eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.222-232.1992
更新日期:1992-01-01 00:00:00
abstract::A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7138-7141.1993
更新日期:1993-11-01 00:00:00
abstract::Shewanella oneidensis MR-1 is a facultatively anaerobic bacterium capable of using soluble and insoluble forms of manganese [Mn(III/IV)] and iron [Fe(III)] as terminal electron acceptors during anaerobic respiration. To assess the structural association of two outer membrane-associated c-type decaheme cytochromes (i.e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01966-05
更新日期:2006-07-01 00:00:00
abstract::Heterocyst differentiation is orchestrated by the N control transcriptional regulator NtcA and the differentiation-specific factor HetR. In Anabaena sp. strain PCC 7120, the devBCA operon is expressed from two different promoters activated upon N stepdown. The distal devB promoter (transcription start point [TSP] loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05994-11
更新日期:2012-06-01 00:00:00
abstract::Significant changes in the relative proportions of the individual polar lipids of two strains of Bacillus subtilis were observed when the pH of their chemostat cultures was varied. In phosphate- and magnesium-limited cultures of B. subtilis var. niger NCIB 8058. lysylphosphatidylglycerol was present in higher proporti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.3.999-1003.1974
更新日期:1974-12-01 00:00:00
abstract::Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.673-684.1973
更新日期:1973-11-01 00:00:00
abstract::Caldwell, D. R. (U.S. Department of Agriculture, Beltsville, Md.), D. C. White, M. P. Bryant, and R. N. Doetsch. Specificity of the heme requirement for growth of Bacteroides ruminicola. J. Bacteriol. 90:1645-1654. 1965.-Previous studies suggested that most strains of Bacteroides ruminicola subsp. ruminicola require h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.6.1645-1654.1965
更新日期:1965-12-01 00:00:00
abstract::soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.9.2864-2871.1991
更新日期:1991-05-01 00:00:00
abstract::We have previously cloned the genes for synthesis of capsular polysaccharide (cps) and slime from Erwinia stewartii in cosmid pES2144. In this study, pES2144 was shown to complement 14 spontaneous cps mutants. These mutants were characterized by probing Southern blots of mutant genomic DNA with pES2144; insertions wer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.865-871.1988
更新日期:1988-02-01 00:00:00
abstract::Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.895-900.1963
更新日期:1963-04-01 00:00:00
abstract::Two bacteriophage BF23 late genes, genes 24 and 25, were isolated on a 7.4-kb PstI fragment from the phage DNA, and their nucleotide sequences were determined. Gene 24 encodes a minor tail protein with the expected M(r) of 34,309, and gene 25 located 4 bp upstream of gene 24 encodes a major tail protein with the expec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7280-7290.1994
更新日期:1994-12-01 00:00:00
abstract::Mycoplasma showing at least two colony types were isolated from the nares and oropharynx of New Zealand white rabbits. Two strains were purified by single-colony passages and characterized. Morphology by phase-contrast and electron microscopy was typical of Mycoplasmataceae. Both grew anaerobically as well as aerobica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.4.1416-1424.1967
更新日期:1967-04-01 00:00:00
abstract::The bacteriophage lambda cIII gene product regulates the lysogenic pathway. The cIII gene is located in the leftward operon, which is transcribed from the pL promoter. We have previously shown (S. Altuvia and A. B. Oppenheim, J. Bacteriol. 167:415-419, 1986) that mutations that show elevated expression lie within the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2563-2572.1989
更新日期:1989-05-01 00:00:00
abstract::Short-chain acid-soluble polyphosphates were extracted from growing cultures of Saccharomyces cerevisiae, and the changes in the levels of these compounds were determined. The production of acid-soluble polyphosphates correlated with the mitochondrial activities since it occurred in two bursts in respiration-competent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.710-714.1980
更新日期:1980-08-01 00:00:00
abstract::The discovery of a novel multicistronic operon that encodes phosphofructokinase, pyruvate kinase, and lactate dehydrogenase in the lactic acid bacterium Lactococcus lactis is reported. The three genes in the operon, designated pfk, pyk, and ldh, contain 340, 502, and 325 codons, respectively. The intergenic distances ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2541-2551.1993
更新日期:1993-05-01 00:00:00
abstract::Chlamydia trachomatis lacks the canonical genes required for the biosynthesis of p-aminobenzoate (pABA), a component of essential folate cofactors. Previous studies revealed a single gene from C. trachomatis, the CT610 gene, that rescues Escherichia coli ΔpabA, ΔpabB, and ΔpabC mutants, which are otherwise auxotrophic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00319-20
更新日期:2020-09-23 00:00:00
abstract::Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.468-478.1979
更新日期:1979-11-01 00:00:00
abstract::The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.20.6286-6294.1994
更新日期:1994-10-01 00:00:00
abstract::Strains missing several genes required for chemotaxis toward amino acids, peptides, and certain sugars were tethered and their rotational behavior was analyzed. Null strains (called gutted) were deleted for genes that code for the transducers Tsr, Tar, Tap, and Trg and for the cytoplasmic proteins CheA, CheW, CheR, Ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.1878-1885.1987
更新日期:1987-05-01 00:00:00
abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00373-15
更新日期:2016-01-11 00:00:00
abstract::Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06603-11
更新日期:2012-03-01 00:00:00
abstract::To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.1.280-283.1997
更新日期:1997-01-01 00:00:00
abstract::We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopalin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.903-905.1991
更新日期:1991-01-01 00:00:00
abstract::EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.8.3110-3115.2006
更新日期:2006-04-01 00:00:00
abstract::The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.20.5533-5544.2002
更新日期:2002-10-01 00:00:00
abstract::Adenosine 5'-triphosphate (ATP) synthesis driven by an artificially imposed membrane potential in right-side-out membrane vesicles of Escherichia coli was investigated. Membrane vesicles prepared in the presence of adenosine diphosphate were loaded with K+ by incubation with 0.5 M potassium phosphate. Addition of vali...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.154-161.1976
更新日期:1976-07-01 00:00:00
abstract::Autotransporter biogenesis is dependent upon BamA, a central component of the β-barrel assembly machinery (BAM) complex. In this report, we detail the role of the other BAM components (BamB-E). We identify the importance of BamD in autotransporter biogenesis and show that BamB, BamC, and BamE are not required. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00192-11
更新日期:2011-08-01 00:00:00
abstract::The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100 and purified as de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1752-1758.1988
更新日期:1988-04-01 00:00:00
abstract::Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.1.219-223.1977
更新日期:1977-10-01 00:00:00
abstract::We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2612-2619.1992
更新日期:1992-04-01 00:00:00