Abstract:
:Autotransporter biogenesis is dependent upon BamA, a central component of the β-barrel assembly machinery (BAM) complex. In this report, we detail the role of the other BAM components (BamB-E). We identify the importance of BamD in autotransporter biogenesis and show that BamB, BamC, and BamE are not required.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rossiter AE,Leyton DL,Tveen-Jensen K,Browning DF,Sevastsyanovich Y,Knowles TJ,Nichols KB,Cunningham AF,Overduin M,Schembri MA,Henderson IRdoi
10.1128/JB.00192-11subject
Has Abstractpub_date
2011-08-01 00:00:00pages
4250-3issue
16eissn
0021-9193issn
1098-5530pii
JB.00192-11journal_volume
193pub_type
杂志文章abstract::Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, during vegetative growth. The CwlF protein was extracted from B. subtilis cwlB sigD mutant cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequencing revealed that its sequence is completely identical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.9.2549-2555.1998
更新日期:1998-05-01 00:00:00
abstract::Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.1281-1284.1968
更新日期:1968-10-01 00:00:00
abstract::Plasmid R1 is a low-copy-number plasmid that is present at a level of about four or five copies per average cell. The copy number is controlled posttranscriptionally at the level of synthesis of the rate-limiting initiator protein RepA. In addition to this, R1 has an auxiliary system that derepresses a second promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.1.207-211.2004
更新日期:2004-01-01 00:00:00
abstract::Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.1225-1235.1967
更新日期:1967-10-01 00:00:00
abstract::Antiserum was made against a single 30S protein, 30S-7. The amount of complement fixed by total 30S protein in the presence of this antiserum indicated that protein 30S-7 was the major antigen in the mixture of proteins. Each of the 30S ribosomal proteins was tested for cross-reactivity with anti-30S-7. This was done ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.2.910-915.1971
更新日期:1971-11-01 00:00:00
abstract::Under starvation conditions, a swarm of Myxococcus xanthus cells will undergo development, a multicellular process culminating in the formation of many aggregates called fruiting bodies, each of which contains up to 100,000 spores. The mechanics of symmetry breaking and the self-organization of cells into fruiting bod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05188-11
更新日期:2011-10-01 00:00:00
abstract::The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00655-07
更新日期:2007-08-01 00:00:00
abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02169-12
更新日期:2013-07-01 00:00:00
abstract::The kps locus for polysialic acid capsule expression in Escherichia coli K1 is composed of a central group of biosynthetic neu genes, designated region 2, flanked on either side by region 1 or region 3 kps genes with poorly defined functions. Chromosomal mutagenesis with MudJ and subsequent complementation analysis, m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.8018-8023.1993
更新日期:1993-12-01 00:00:00
abstract::The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.16.4970-4978.1993
更新日期:1993-08-01 00:00:00
abstract::Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2160-2166.1998
更新日期:1998-04-01 00:00:00
abstract::The Salmonella effector protein SopA is translocated into host cells via the SPI-1 type III secretion system (TTSS) and contributes to enteric disease. We found that the chaperone InvB binds to SopA and slightly stabilizes it in the bacterial cytosol and that it is required for its transport via the SPI-1 TTSS. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1215-1219.2004
更新日期:2004-02-01 00:00:00
abstract::The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA). Compared with isogenic rep(+) strains, rep mutants show the following characteristics: larger cell size, more DNA per cell, and a slightly lower DNA/mass ratio. The replicating rep ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.805-814.1974
更新日期:1974-11-01 00:00:00
abstract::Upon induction, Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills B. megaterium and a few other bacterial species. The DNA region responsible for megacinogeny was cloned in B. megaterium. The nucleotide sequence of a 5,494-bp-long subfragment was determ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00557-08
更新日期:2008-10-01 00:00:00
abstract::Shewanella putrefaciens strain 200 respires a wide range of compounds as terminal electron acceptor. The respiratory versatility of Shewanella is attributed in part to a set of c-type cytochromes with widely varying midpoint redox potentials (E'(0)). A point mutant of S. putrefaciens, originally designated Urr14 and h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01249-06
更新日期:2007-02-01 00:00:00
abstract::We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00044-07
更新日期:2007-05-01 00:00:00
abstract::Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.10.3438-3444.2005
更新日期:2005-05-01 00:00:00
abstract::An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.24.7001-7012.2002
更新日期:2002-12-01 00:00:00
abstract::A second synchrony induction treatment, half-way through the deoxyribonucleic acid replication process of an already synchronized culture, resulted in defective subsequent cell growth. This supports a previous explanation for the partial loss of viability after synchrony induction. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.583-584.1971
更新日期:1971-08-01 00:00:00
abstract::Prokaryotic organisms occupy the most diverse set of environments and conditions on our planet. Their ability to sense and respond to a broad range of external cues remain key research areas in modern microbiology, central to behaviors that underlie beneficial and pathogenic interactions of bacteria with multicellular...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00439-19
更新日期:2019-09-20 00:00:00
abstract::A denitrifying Pseudomonas sp. is able to oxidize aromatic compounds compounds completely to CO2, both aerobically and anaerobically. It is shown that benzoate is aerobically oxidized by a new degradation pathway via benzoyl-coenzyme A (CoA) and 3-hydroxybenzoyl-CoA. The organism grew aerobically with benzoate, 3-hydr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4851-4858.1993
更新日期:1993-08-01 00:00:00
abstract::Type 1 fimbriae and flagella have been previously shown to contribute to the virulence of uropathogenic Escherichia coli (UPEC) within the urinary tract. In this study, the relationship between motility and type 1 fimbrial expression was tested for UPEC strain CFT073 by examining the phenotypic effect of fimbrial expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00434-07
更新日期:2007-08-01 00:00:00
abstract::NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.19.6661-6666.2004
更新日期:2004-10-01 00:00:00
abstract::Using a quantitative assay for measuring chemotaxis, we tested a variety of sugars and sugar derivatives for their ability to attract Escherichia coli bacteria. The most effective attractants, i.e., those that have thresholds near 10(-5) M or below, are N-acetyl-d-glucosamine, 6-deoxy-d-glucose, d-fructose, d-fucose, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.824-847.1973
更新日期:1973-09-01 00:00:00
abstract::Expression of the Bacillus licheniformis alpha-amylase gene, amyL, was temporally activated and subject to catabolite repression both in its natural host and when cloned on a 3.55-kilobase fragment in Bacillus subtilis. A subclone from which the promoter region of amyL and sequences upstream from the promoter were del...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2435-2442.1989
更新日期:1989-05-01 00:00:00
abstract::The origin of replication of the plasmid R1162 contains an initiation site for the synthesis of each DNA strand. When one of these sites (oriL) is deleted, synthesis on the corresponding strand is no longer initiated efficiently in vitro by the R1162-encoded replication proteins, and the plasmid is no longer stably ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.16.4870-4876.1996
更新日期:1996-08-01 00:00:00
abstract::Lambda Xis, which is required for site-specific excision of phage lambda from the bacterial chromosome, has a much shorter functional half-life than Int, which is required for both integration and excision (R. A. Weisberg and M. E. Gottesman, p. 489-500, in A. D. Hershey, ed., The Bacteriophage Lambda, 1971). We found...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.6.1573-1577.1998
更新日期:1998-03-01 00:00:00
abstract::The glnB gene from Bradyrhizobium japonicum, the endosymbiont of soybeans (Glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. The B. japonicum glnB gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnB gene product...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5638-5645.1989
更新日期:1989-10-01 00:00:00
abstract::Several characteristics of Mycobacterium tuberculosis (e.g., conserved genome and low growth rate) have severely restricted the study of the microorganism. The discovery of IS6110 raised hopes of overcoming these obstacles. However, our knowledge of this IS element is relatively limited; even its two basic characteris...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.18.5279-5284.2001
更新日期:2001-09-01 00:00:00
abstract::The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05862-11
更新日期:2011-10-01 00:00:00