Cloning, sequencing, expression, and complementation analysis of the Escherichia coli K1 kps region 1 gene, kpsE, and identification of an upstream open reading frame encoding a protein with homology to GutQ.

abstract：:Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and...

journal_title：Journal of bacteriology

authors： Kline BC,Schoenhard DE

更新日期：1969-10-01 00:00:00

abstract：:Fine details of the internal and external morphology of Cryptococcus neoformans as seen in ultrathin sections are described and illustrated with electron micrographs. The capsule characteristic of this species contained microfibrils (30 to 40 A in diameter) that appeared to radiate from the cell wall and to coil and i...

journal_title：Journal of bacteriology

authors： Edwards MR,Gordon MA,Lapa EW,Ghiorse WC

更新日期：1967-09-01 00:00:00

abstract：:Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...

journal_title：Journal of bacteriology

authors： Kang J,Blaser MJ

更新日期：2008-06-01 00:00:00

abstract：:Catabolism of nonphenolic arenes is frequently initiated by dioxygenases, yielding single isomer products with two adjacent hydroxylated asymmetric centers. The next enzymic reaction dehydrogenates these cyclic cis-diols, with aromatization yielding catechols for ring cleavage. There are two stereochemical questions t...

journal_title：Journal of bacteriology

authors： Morawski B,Casy G,Illaszewicz C,Griengl H,Ribbons DW

更新日期：1997-06-01 00:00:00

abstract：:Bulen, William A. (Charles F. Kettering Foundation, Yellow Springs, Ohio). Effect of tungstate on the uptake and function of molybdate in Azotobacter agilis. J. Bacteriol. 82:130-134. 1961.-The reported competitive inhibition of molybdate by tungstate was investigated in an effort to elucidate molybdenum functions ass...

journal_title：Journal of bacteriology

authors： Bulen WA

更新日期：1961-07-01 00:00:00

abstract：:Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

journal_title：Journal of bacteriology

authors： ELLNER PD,BOHAN CD

更新日期：1962-02-01 00:00:00

abstract：:The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the...

journal_title：Journal of bacteriology

authors： Zupan J,Hackworth CA,Aguilar J,Ward D,Zambryski P

更新日期：2007-09-01 00:00:00

abstract：:An earlier study of the regulation of glutamate synthase (GOGAT) in Bacillus subtilis (Deshpande et al., Bichem. Biophys. Res. Commun. 95:55--60, 1980) revealed an inverse relationship between the specific activity of this essential ammonia-assimilatory enzyme and the intracellular pool of glutamine: GOGAT activity de...

journal_title：Journal of bacteriology

authors： Deshpande KL,Katze JR,Kane JF

更新日期：1981-02-01 00:00:00

abstract：:We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expr...

journal_title：Journal of bacteriology

authors： Membrillo-Hernández J,Kim SO,Cook GM,Poole RK

更新日期：1997-05-01 00:00:00

abstract：:Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...

journal_title：Journal of bacteriology

authors： Donald RG,Lapointe J,Ludwig RA

更新日期：1988-03-01 00:00:00

abstract：:Significant changes in the relative proportions of the individual polar lipids of two strains of Bacillus subtilis were observed when the pH of their chemostat cultures was varied. In phosphate- and magnesium-limited cultures of B. subtilis var. niger NCIB 8058. lysylphosphatidylglycerol was present in higher proporti...

journal_title：Journal of bacteriology

authors： Minnikin DE,Abdolrahimzadeh H

更新日期：1974-12-01 00:00:00

abstract：:Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization ...

journal_title：Journal of bacteriology

authors： Dietrich SM

更新日期：1976-09-01 00:00:00

abstract：:Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...

journal_title：Journal of bacteriology

authors： de Bruijn I,de Kock MJ,de Waard P,van Beek TA,Raaijmakers JM

更新日期：2008-04-01 00:00:00

abstract：:In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...

journal_title：Journal of bacteriology

authors： Ballesta JP,De Garcia CL,Schaechter M

更新日期：1973-10-01 00:00:00

abstract：:We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) and fastidious, obligately microaerophilic species, such as Magnetospirillum magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from strains MGT-1 and AMB-1 was used as ...

journal_title：Journal of bacteriology

authors： Burgess JG,Kawaguchi R,Sakaguchi T,Thornhill RH,Matsunaga T

更新日期：1993-10-01 00:00:00

abstract：:trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and transl...

journal_title：Journal of bacteriology

authors： Yang M,de Saizieu A,van Loon AP,Gollnick P

更新日期：1995-08-01 00:00:00

abstract：:Lipoprotein biogenesis in Gram-negative bacteria occurs by a conserved pathway, each step of which is considered essential. In contrast to this model, LoVullo and colleagues demonstrate that the N-acyl transferase Lnt is not required in Francisella tularensis or Neisseria gonorrhoeae. This suggests the existence of a ...

journal_title：Journal of bacteriology

authors： Chahales P,Thanassi DG

更新日期：2015-05-01 00:00:00

abstract：:The activity of the toluene-responsive sigma(54) Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when ame...

journal_title：Journal of bacteriology

authors： Cases I,de Lorenzo V

更新日期：2000-02-01 00:00:00

abstract：:RSF1010 plasmid DNA was introduced into Pseudomonas putida and P. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in Pseudomonas species. The number of copies of RSF1010 was 43 per chromosome equivalent in P. putida cells. ...

journal_title：Journal of bacteriology

authors： Nagahari K,Sakaguchi K

更新日期：1978-03-01 00:00:00

abstract：:Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...

journal_title：Journal of bacteriology

authors： Shao CP,Lo HR,Lin JH,Hor LI

更新日期：2011-05-01 00:00:00

abstract：:Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...

journal_title：Journal of bacteriology

authors： Oh HM,Giovannoni SJ,Ferriera S,Johnson J,Cho JC

更新日期：2009-04-01 00:00:00

abstract：:Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. Th...

journal_title：Journal of bacteriology

authors： Paustian ML,May BJ,Cao D,Boley D,Kapur V

更新日期：2002-12-01 00:00:00

abstract：:A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all ...

journal_title：Journal of bacteriology

authors： Plazinski J,Dart PJ,Rolfe BG

更新日期：1983-09-01 00:00:00

abstract：:It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....

journal_title：Journal of bacteriology

authors： Sikora AE,Zielke R,Datta K,Maddock JR

更新日期：2006-02-01 00:00:00

abstract：UNLABELLED:Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetyl...

journal_title：Journal of bacteriology

authors： Uhde A,Brühl N,Goldbeck O,Matano C,Gurow O,Rückert C,Marin K,Wendisch VF,Krämer R,Seibold GM

更新日期：2016-07-28 00:00:00

abstract：:Membrane vesicles isolated from cells of bacillus subtilis W23 accumulate manganese in the presence of an energy source. The artificial electron donor system ascorbate and phenazine methosulfate or reduced nicotinamide adenine dinucleotide and phenazine methosulfate can supply the energy for the uptake. D-Lactate in t...

journal_title：Journal of bacteriology

authors： Bhattacharyya P

更新日期：1975-07-01 00:00:00

abstract：:Glycerol:NAD+2-OXIDOREDUCTASE (EC 1.1.1.6) was purified to homogeneity from a mutant of Escherichia coli K12 that uses this enzyme, instead of ATP:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sul...

journal_title：Journal of bacteriology

authors： Tang CT,Ruch FE Jr,Lin CC

更新日期：1979-10-01 00:00:00

abstract：:A detailed study of the cultural characteristics and cellular fatty acid composition of 27 isolates of Corynebacterium acnes was performed to establish the properties by which this organism may be identified and characterized. The fatty acids were extracted directly from whole cells and examined as methyl esters by ga...

journal_title：Journal of bacteriology

authors： Moss CW,Dowell VR Jr,Lewis VJ,Schekter MA

更新日期：1967-11-01 00:00:00

abstract：:The major outer membrane lipoprotein (Lpp) of Escherichia coli possesses serine at position 2, which is thought to function as the outer membrane sorting signal, and lysine at the C terminus, through which Lpp covalently associates with peptidoglycan. Arginine (R) is present before the C-terminal lysine in the wild-ty...

journal_title：Journal of bacteriology

authors： Yakushi T,Tajima T,Matsuyama S,Tokuda H

更新日期：1997-05-01 00:00:00

abstract：:Integrons constitute a novel family of DNA elements which evolved by site-specific integration of discrete units between two conserved segments. On the In4 integron of Tn1696, a precisely inserted gene cassette of 1,549 bp conferring nonenzymatic chloramphenicol resistance (cmlA) is present between the streptomycin-sp...

journal_title：Journal of bacteriology

authors： Bissonnette L,Champetier S,Buisson JP,Roy PH

更新日期：1991-07-01 00:00:00