Method for restricting incorporation of radioactivity from 3 H-thymidine into deoxyribonucleic acid only during outgrowth of spores of Bacillus cereus T.

Abstract:

:When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine into DNA only. This was accomplished by labeling the cells with (3)H-thymidine in the presence of 2 mg of 2-deoxyadenosine per ml, 250 mug each of uracil, cytosine, and guanosine per ml, and 500 mug of adenosine per ml. Under these conditions, 97% of the radioactivity incorporated into cold trichloroacetic acid-insoluble material was associated with DNA only. In the absence of these compounds, DNA contained only 72% of the total radioactivity incorporated into cold acid-insoluble material.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rana RS,Halvorson HO

doi

10.1128/JB.109.2.599-605.1972

subject

Has Abstract

pub_date

1972-02-01 00:00:00

pages

599-605

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

109

pub_type

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