Escherichia coli strains blocked in Tat-dependent protein export exhibit pleiotropic defects in the cell envelope.

Abstract:

:The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. Mutant strains are also hypersensitive to hydrophobic drugs and to lysis by lysozyme in the absence of EDTA, and they leak periplasmic enzymes, characteristics that are consistent with an outer membrane defect. Both phenotypes are similar to those displayed by strains carrying point mutations in the lpxC (envA) gene. The phenotype was not replicated by mutations affecting synthesis and/or activity of all known or predicted Tat substrates.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Stanley NR,Findlay K,Berks BC,Palmer T

doi

10.1128/JB.183.1.139-144.2001

keywords:

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

139-44

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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