Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei.

abstract：:A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA enco...

journal_title：Journal of bacteriology

authors： Wilson RL,Steiert PS,Stauffer GV

更新日期：1993-02-01 00:00:00

abstract：:Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl. The optimum incubation time was 1 h, and the optimum cell concen...

journal_title：Journal of bacteriology

authors： Ito H,Fukuda Y,Murata K,Kimura A

更新日期：1983-01-01 00:00:00

abstract：:MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix. This finding has led to a model in which the binding of ATP regulates the formation ...

journal_title：Journal of bacteriology

authors： Zhou H,Lutkenhaus J

更新日期：2003-08-01 00:00:00

abstract：:Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...

journal_title：Journal of bacteriology

authors： Eberz G,Eitinger T,Friedrich B

更新日期：1989-03-01 00:00:00

abstract：:Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature s...

journal_title：Journal of bacteriology

authors： Eller C,Ordal ZJ

更新日期：1972-09-01 00:00:00

abstract：:The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...

journal_title：Journal of bacteriology

authors： Huang L,Tsui P,Freundlich M

更新日期：1992-02-01 00:00:00

abstract：:The formation of recombinants in Hfr crosses was studied in Escherichia coli strains carrying combinations of genes known to affect recombination and DNA repair. Mutations in ruv and recG eliminate activities that have been shown to process Holliday junction intermediates by nuclease cleavage and/or branch migration. ...

journal_title：Journal of bacteriology

authors： Ryder L,Whitby MC,Lloyd RG

更新日期：1994-03-01 00:00:00

abstract：:Trichloroethylene (TCE) is the most frequently detected groundwater contaminant, and 1-naphthol is an important chemical manufacturing intermediate. Directed evolution was used to increase the activity of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 for both chlorinated ethenes and naphthalene oxidatio...

journal_title：Journal of bacteriology

authors： Canada KA,Iwashita S,Shim H,Wood TK

更新日期：2002-01-01 00:00:00

abstract：:A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

journal_title：Journal of bacteriology

authors： Hall BG

更新日期：1999-02-01 00:00:00

abstract：:Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

journal_title：Journal of bacteriology

authors： Fitz-James PC

更新日期：1971-03-01 00:00:00

abstract：:During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...

journal_title：Journal of bacteriology

authors： Severinova E,Severinov K

更新日期：2006-05-01 00:00:00

abstract：:The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treat...

journal_title：Journal of bacteriology

authors： Sonnenfeld EM,Koch AL,Doyle RJ

更新日期：1985-09-01 00:00:00

abstract：:We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 protein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environm...

journal_title：Journal of bacteriology

authors： Lee JH,Koh HY,Lee SG,Doyle S,Christner BC,Kim HJ

更新日期：2012-12-01 00:00:00

abstract：:Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...

journal_title：Journal of bacteriology

authors： Copeland JC

更新日期：1971-02-01 00:00:00

abstract：:Livers of uninfected guinea pigs and of guinea pigs infected with Coxiella burneti were fractionated into smooth endoplasmic reticulum, rough endoplasmic reticulum (RER), pellet, and cell sap fractions. The ribonucleic acid (RNA) and protein of each fraction were determined, and the phosphorylase, glucose-6-phosphatas...

journal_title：Journal of bacteriology

authors： Paretsky D,Stueckemann J

更新日期：1970-05-01 00:00:00

abstract：:lisRK encodes a two-component regulatory system in the food pathogen Listeria monocytogenes LO28. Following identification of the operon in an acid-tolerant Tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol c...

journal_title：Journal of bacteriology

authors： Cotter PD,Emerson N,Gahan CG,Hill C

更新日期：1999-11-01 00:00:00

abstract：:In wild-type Escherichia coli, fumarate reductase encoded by the frd operon is inducible by its substrate in the absence of molecular oxygen and nitrate. Synthesis of this enzyme under permissive conditions requires the fnr+ gene product, which is believed to be a pleiotropic regulatory protein that activates transcri...

journal_title：Journal of bacteriology

authors： Iuchi S,Kuritzkes DR,Lin EC

更新日期：1985-03-01 00:00:00

abstract：:Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

journal_title：Journal of bacteriology

authors： Tisa LS,Ensign JC

更新日期：1988-07-01 00:00:00

abstract：:The relationship between specific growth rate of Escherichia coli and the concentration of limiting nutrient (glucose or phosphate or tryptophan) has been determined for populations in a steady state. At high concentrations the specific growth rate is independent of the concentration of nutrient, but at low concentrat...

journal_title：Journal of bacteriology

authors： Shehata TE,Marr AG

更新日期：1971-07-01 00:00:00

abstract：:The protobranch bivalve Solemya velum Say (Mollusca: Bivalvia) houses chemoautotrophic symbionts intracellularly within its gills. These symbionts were characterized through sequencing of polymerase chain reaction-amplified 16S rRNA coding regions and hybridization of an Escherichia coli gene probe to S. velum genomic...

journal_title：Journal of bacteriology

authors： Eisen JA,Smith SW,Cavanaugh CM

更新日期：1992-05-01 00:00:00

abstract：:Salmonella virulence requires the initial invasion of host cells, followed by modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in Salmonella pathogenesis, we inadvertently identified a 5-kDa protein named YshB that is involved in the intracel...

journal_title：Journal of bacteriology

authors： Bomjan R,Zhang M,Zhou D

更新日期：2019-08-08 00:00:00

abstract：:The gene specifying a membrane-bound nucleoside diphosphate sugar hydrolase of Salmonella typhimurium was mapped near the metA locus by using intergeneric crosses between this bacterium and Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Minton N,Gunn J,Beacham IR

更新日期：1979-03-01 00:00:00

abstract：:Photobacterium leiognathi ATCC 25521 (the type strain and light-organ symbiont of ponyfish) is one of the few bacteria that produces a copper-zinc superoxide dismutase, termed bacteriocuprein. We enzymologically and immunologically characterized the bacteriocuprein superoxide dismutases in sonicates from the type stra...

journal_title：Journal of bacteriology

authors： Dunlap PV,Steinman HM

更新日期：1986-02-01 00:00:00

abstract：:Staphylococcus pseudintermedius is a member of the coagulase-positive staphylococci and is the commonest cause of canine pyoderma. We report the first genome sequence of S. pseudintermedius, which shows the presence of numerous virulence factors akin to those of the related human pathogen Staphylococcus aureus. ...

journal_title：Journal of bacteriology

authors： Tse H,Tsoi HW,Leung SP,Urquhart IJ,Lau SK,Woo PC,Yuen KY

更新日期：2011-04-01 00:00:00

abstract：:Mutation of a conserved His-157 in the second pore loop of KtrB drastically reduced the activity of the K+ transporter from Synechocystis sp. strain PCC 6803. This result suggests that His-157 plays an essential role in the K+ transport activity of the transporter system. ...

journal_title：Journal of bacteriology

authors： Zulkifli L,Uozumi N

更新日期：2006-11-01 00:00:00

abstract：:Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling stu...

journal_title：Journal of bacteriology

authors： Webb E,Claas K,Downs DM

更新日期：1997-07-01 00:00:00

abstract：:Cellulolytic strains of Bacillus stearothermophilus were isolated from nature and screened for the presence of activities associated with the degradation of plant cell walls. One isolate (strain XL-65-6) which exhibited strong activities with 4-methylumbelliferyl-beta-D-glucopyranoside (MUG) and 4-methylumbelliferyl-b...

journal_title：Journal of bacteriology

authors： Lai X,Ingram LO

更新日期：1993-10-01 00:00:00

abstract：:The catB and catC genes encode cis,cis-muconate lactonizing enzyme I (EC 5.5.1.1) and muconolactone isomerase (EC 5.3.3.4), respectively. These enzymes are required for the dissimilation of benzoate to beta-ketoadipate by Pseudomonas putida and are under coordinate transcriptional regulation. By deletion analysis and ...

journal_title：Journal of bacteriology

authors： Aldrich TL,Chakrabarty AM

更新日期：1988-03-01 00:00:00

abstract：:A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...

journal_title：Journal of bacteriology

authors： Jarvis BW,Dworkin M

更新日期：1989-09-01 00:00:00

abstract：:d-Desthiobiotin synthetase, an enzyme that catalyzes the synthesis of d-desthiobiotin from dl-7,8-diaminopelargonic acid and HCO(3) (-), was purified 100-fold from cells of a biotin mutant strain of Escherichia coli. Adenosine triphosphate and Mg(2+) were shown, especially in purified extracts, to be obligatory for en...

journal_title：Journal of bacteriology

authors： Cheeseman P,Pai CH

更新日期：1970-11-01 00:00:00