The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.

Abstract:

:Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA may facilitate initiation in the absence of formylation. In this report we show, by using trmA strains of Escherichia coli (defective for rT modification) and a sensitive in vivo initiation assay system, that the lack of rT modification in the initiators is not sufficient to effect formylation-independent initiation of protein synthesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Thanedar S,Dineshkumar TK,Varshney U

doi

10.1128/JB.183.24.7397-7402.2001

keywords:

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

7397-402

issue

24

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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