Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

abstract：:Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and her...

journal_title：Journal of bacteriology

authors： Gardner SP,Olson JW

更新日期：2018-10-10 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, IRE1 encodes a bifunctional protein with transmembrane kinase and endoribonuclease activities. HAC1 encodes a transcription factor which has a basic leucine zipper domain. Both gene products play a crucial role in the unfolded protein response. Mutants in which one of these genes...

journal_title：Journal of bacteriology

authors： Sugiyama M,Nikawa J

更新日期：2001-09-01 00:00:00

abstract：:Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...

journal_title：Journal of bacteriology

authors： Monjarás Feria J,García-Gómez E,Espinosa N,Minamino T,Namba K,González-Pedrajo B

更新日期：2012-11-01 00:00:00

abstract：:A novel sucrose hydrolase (SUH) from Xanthomonas axonopodis pv. glycines, a causative agent of bacterial pustule disease on soybeans, was studied at the functional and molecular levels. SUH was shown to act rather specifically on sucrose (K(m) = 2.5 mM) but not on sucrose-6-phosphate. Protein analysis of purified SUH ...

journal_title：Journal of bacteriology

authors： Kim HS,Park HJ,Heu S,Jung J

更新日期：2004-01-01 00:00:00

abstract：:Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

journal_title：Journal of bacteriology

authors： Stubbs JD,Stubbs EA

更新日期：1971-12-01 00:00:00

abstract：:The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, ...

journal_title：Journal of bacteriology

authors： Ullmann WW,Cameron JA

更新日期：1969-05-01 00:00:00

abstract：:The Escherichia coli K-12 hisT gene has been cloned, and its organization and expression have been analyzed on multicopy plasmids. The hisT gene, which encodes tRNA pseudouridine synthase I (PSUI), was isolated on a Clarke-Carbon plasmid known to contain the purF gene. The presence of the hisT gene on this plasmid was...

journal_title：Journal of bacteriology

authors： Marvel CC,Arps PJ,Rubin BC,Kammen HO,Penhoet EE,Winkler ME

更新日期：1985-01-01 00:00:00

abstract：:A method of assay of immunogenic potency of the cholera gut toxin is described; it is based on the relation of dose of antigen to neutralizing antibody titer produced in the rabbit under defined conditions and allows quantification of immunogenicity as immunogenic units per milligram of protein. Evidence, based on imm...

journal_title：Journal of bacteriology

authors： Kaur J,Burrows W,Cercavski L

更新日期：1969-11-01 00:00:00

abstract：:Bacillus thuringiensis var. thuringiensis Berliner grown at 14 C on a chemically defined liquid medium slowly produced spores and parasporal inclusions. Sporulation ultimately attained 50 to 100% of that of cultures grown at 30 C. ...

journal_title：Journal of bacteriology

authors： Aronson JN,Thompson FM

更新日期：1971-01-01 00:00:00

abstract：:The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were...

journal_title：Journal of bacteriology

authors： Kasian PA,Pittard J

更新日期：1984-10-01 00:00:00

abstract：:The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma ...

journal_title：Journal of bacteriology

authors： Donà V,Rodrigue S,Dainese E,Palù G,Gaudreau L,Manganelli R,Provvedi R

更新日期：2008-09-01 00:00:00

abstract：:Carotovoricin Er is a phage-tail-like bacteriocin produced by Erwinia carotovora subsp. carotovora strain Er, a causative agent for soft rot disease in plants. Here we studied binding and killing spectra of carotovoricin Er preparations for various strains of the bacterium (strains 645Ar, EC-2, N786, and P7) and found...

journal_title：Journal of bacteriology

authors： Nguyen HA,Tomita T,Hirota M,Kaneko J,Hayashi T,Kamio Y

更新日期：2001-11-01 00:00:00

abstract：:Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on ...

journal_title：Journal of bacteriology

authors： Tochikubo K

更新日期：1974-03-01 00:00:00

abstract：:Two primary alcohols (1-butanol and ethanol) are major fermentation products of several clostridial species. In addition to these two alcohols, the secondary alcohol 2-propanol is produced to a concentration of about 100 mM by some strains of Clostridium beijerinckii. An alcohol dehydrogenase (ADH) has been purified t...

journal_title：Journal of bacteriology

authors： Ismaiel AA,Zhu CX,Colby GD,Chen JS

更新日期：1993-08-01 00:00:00

abstract：:The pelA and pelC genes from Erwinia chrysanthemi EC16 were sequenced and overexpressed in Escherichia coli cells. These genes and two others from the same strain that were characterized previously encode catalytically related pectate lyase proteins that are involved with the maceration and soft-rotting of plant tissu...

journal_title：Journal of bacteriology

authors： Tamaki SJ,Gold S,Robeson M,Manulis S,Keen NT

更新日期：1988-08-01 00:00:00

abstract：:We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...

journal_title：Journal of bacteriology

authors： Mekalanos JJ,Sublett RD,Romig WR

更新日期：1979-09-01 00:00:00

abstract：:Dienelactone hydrolases have previously been shown to play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. Recently, the enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelact...

journal_title：Journal of bacteriology

authors： Schlömann M,Ngai KL,Ornston LN,Knackmuss HJ

更新日期：1993-05-01 00:00:00

abstract：:CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...

journal_title：Journal of bacteriology

authors： Heilpern AJ,Waldor MK

更新日期：2003-02-01 00:00:00

abstract：:The expression of the pur operon, which encodes enzymes of the purine biosynthetic pathway in Bacillus subtilis, is subject to control by the purR gene product (PurR) and phosphoribosylpyrophosphate. This control is also exerted on the purA and purR genes. A consensus sequence for the binding of PurR, named the PurBox...

journal_title：Journal of bacteriology

authors： Saxild HH,Brunstedt K,Nielsen KI,Jarmer H,Nygaard P

更新日期：2001-11-01 00:00:00

abstract：:Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

journal_title：Journal of bacteriology

authors： McCarthy D,Kupfer DM

更新日期：1987-02-01 00:00:00

abstract：:The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, which is catalyzed by 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase). Pathogenic Brucella species adapted to an intr...

journal_title：Journal of bacteriology

authors： Zylberman V,Klinke S,Haase I,Bacher A,Fischer M,Goldbaum FA

更新日期：2006-09-01 00:00:00

abstract：:The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

journal_title：Journal of bacteriology

authors： Mori H,Shimokawa N,Satoh Y,Ito K

更新日期：2004-06-01 00:00:00

abstract：:The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protop...

journal_title：Journal of bacteriology

authors： Jacobs NJ,Jacobs JM,Brent P

更新日期：1970-05-01 00:00:00

abstract：:Adaptation of Lactococcus lactis towards progressive carbon starvation is mediated by three different types of transcriptomic responses: (i) global responses, i.e., general decreases of functions linked to bacterial growth and lack of induction of the general stress response; (ii) specific responses functionally relat...

journal_title：Journal of bacteriology

authors： Redon E,Loubiere P,Cocaign-Bousquet M

更新日期：2005-05-01 00:00:00

abstract：:Pseudomonas aeruginosa and many other bacteria can utilize biogenic polyamines, including diaminopropane (DAP), putrescine (Put), cadaverine (Cad), and spermidine (Spd), as carbon and/or nitrogen sources. Transcriptome analysis in response to exogenous Put and Spd led to the identification of a list of genes encoding ...

journal_title：Journal of bacteriology

authors： Yao X,He W,Lu CD

更新日期：2011-08-01 00:00:00

abstract：:Dormant spores of Bacillus subtilis exhibit two prominent X-ray scattering peaks. These peaks persisted in spores lacking most alpha/beta-type small, acid-soluble protein or the CotE protein responsible for assembly of much spore coat protein, but they were absent from spores of strains lacking the late sporulation-sp...

journal_title：Journal of bacteriology

authors： Qiu X,Setlow P

更新日期：2009-12-01 00:00:00

abstract：:Archaea are abundant and drive critical microbial processes in the Earth's cold biosphere. Despite this, not enough is known about the molecular mechanisms of cold adaptation and no biochemical studies have been performed on stenopsychrophilic archaea (e.g., Methanogenium frigidum). This study examined the structural ...

journal_title：Journal of bacteriology

authors： Giaquinto L,Curmi PM,Siddiqui KS,Poljak A,DeLong E,DasSarma S,Cavicchioli R

更新日期：2007-08-01 00:00:00

abstract：:Ureolytic clinical isolates of Providencia stuartii, Salmonella spp., and some Escherichia coli strains contain large urease-encoding plasmids. Expression of urease activity from these isolates is induced at least 20-fold by urea. In order to facilitate studies on the regulatory mechanism controlling this urea-inducib...

journal_title：Journal of bacteriology

authors： D'Orazio SE,Collins CM

更新日期：1993-06-01 00:00:00

abstract：:To examine the role of the amino acid residues (between positions 258 and 275 and positions 297 and 298) of the alpha-subunit of RNA polymerase in TyrR-mediated activation of the mtr promoter, we have carried out in vitro transcription experiments using a set of mutant RNA polymerases with a supercoiled mtr template. ...

journal_title：Journal of bacteriology

authors： Yang J,Murakami K,Camakaris H,Fujita N,Ishihama A,Pittard AJ

更新日期：1997-10-01 00:00:00

abstract：:Analysis of two virulence mutants of Pseudomonas syringae B728a revealed that the Tn 5 sites of insertion were within the gidA open reading frame (ORF). These mutations were pleiotropic, affecting diverse phenotypic traits, such as lipodepsipeptide (syringomycin and syringopeptin) antibiotic production, swarming, pres...

journal_title：Journal of bacteriology

authors： Kinscherf TG,Willis DK

更新日期：2002-04-01 00:00:00