Electron microscopy of single-stranded structures in the DNA of competent Haemophilus influenzae cells.

Abstract:

:Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence development. The generation of gaps was dependent on the rec-2 function. Since the rec-2 mutant is defective in the translocation of donor DNA, it was inferred that the gaps were involved in the translocation step of transformation. The generation of single-stranded tails was independent of the rec-1 and rec-2 genes. Therefore, these structures were assumed to play no direct role in the interaction of donor and recipient DNAs during transformation. Gaps were preferentially associated with a readily denaturable, possibly A + T-rich fraction of the genome. This finding raised the possibility that hot spots for transformation might be associated with A + T-rich DNA.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

McCarthy D,Kupfer DM

doi

10.1128/jb.169.2.565-571.1987

subject

Has Abstract

pub_date

1987-02-01 00:00:00

pages

565-71

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

杂志文章
  • Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842.

    abstract::pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1058-1062.1976

    authors: Cornelis G,Bennett PM,Grinsted J

    更新日期:1976-09-01 00:00:00

  • SpoVM, a small protein essential to development in Bacillus subtilis, interacts with the ATP-dependent protease FtsH.

    abstract::The spoVM gene encodes a 26-amino-acid polypeptide that is essential for spore formation in Bacillus subtilis. A transposon insertion within the spoVM open reading frame has been shown to encode a chimeric protein which is biologically inactive and produces a phenotype identical to that of a deletion and insertion mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.17.5534-5542.1997

    authors: Cutting S,Anderson M,Lysenko E,Page A,Tomoyasu T,Tatematsu K,Tatsuta T,Kroos L,Ogura T

    更新日期:1997-09-01 00:00:00

  • NIC, a conjugative nicotine-nicotinate degradative plasmid in Pseudomonas convexa.

    abstract::The plasmid nature of genes specifying degradation of nicotine and nicotinate in Pseudomas convexa strain 1 (Pc1) is indicated by mitomycin curing and conjugational transfer to other strains. The NIC plasmid appears to be compatible with other metabolic plasmids in Pseudomonas putida. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.1.289-290.1978

    authors: Thacker R,Rørvig O,Kahlon P,Gunsalus IC

    更新日期:1978-07-01 00:00:00

  • Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.

    abstract::A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.12.4166-4168.1992

    authors: Witkin EM,Roegner-Maniscalco V

    更新日期:1992-06-01 00:00:00

  • Isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12.

    abstract::We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.568-573.1979

    authors: Pacelli LZ,Edmiston SH,Mount DW

    更新日期:1979-01-01 00:00:00

  • Functional analysis of the three TATA binding protein homologs in Methanosarcina acetivorans.

    abstract::The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches. Although tbp2 and tbp3 deletion mutants were readily obtained, a tbp1 mutant was not obtained, and the growth of a conditional tbp1 expre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01165-09

    authors: Reichlen MJ,Murakami KS,Ferry JG

    更新日期:2010-03-01 00:00:00

  • Draft genome sequence of Sporolactobacillus inulinus strain CASD, an efficient D-lactic acid-producing bacterium with high-concentration lactate tolerance capability.

    abstract::Sporolactobacillus inulinus CASD is an efficient D-lactic acid producer with high optical purity. Here we report for the first time the draft genome sequence of S. inulinus (2,930,096 bp). The large number of annotated two-component system genes makes it possible to explore the mechanism of extraordinary lactate toler...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05934-11

    authors: Yu B,Su F,Wang L,Xu K,Zhao B,Xu P

    更新日期:2011-10-01 00:00:00

  • Effect of Lipidation on the Localization and Activity of a Lysozyme Inhibitor in Neisseria gonorrhoeae.

    abstract::The Gram-negative pathogen Neisseria gonorrhoeae (gonococcus [Gc]) colonizes lysozyme-rich mucosal surfaces. Lysozyme hydrolyzes peptidoglycan, leading to bacterial lysis. Gc expresses two proteins, SliC and NgACP, that bind and inhibit the enzymatic activity of lysozyme. SliC is a surface-exposed lipoprotein, while N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00633-19

    authors: Ragland SA,Gray MC,Melson EM,Kendall MM,Criss AK

    更新日期:2020-03-26 00:00:00

  • Direction of deoxyribonucleic acid transfer and replication in a derivative of plasmid R100-1.

    abstract::The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1105-1108.1979

    authors: Legrand P,Bouche JP,Louarn JM

    更新日期:1979-12-01 00:00:00

  • Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among.

    abstract::Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.409-414.1962

    authors: WEISS E,DRESSLER HR

    更新日期:1962-02-01 00:00:00

  • Phospho-beta-glucosidases and beta-glucoside permeases in Streptococcus, Bacillus, and Staphylococcus.

    abstract::The presence of phospho-beta-glucosidases and beta-glucoside permeases was found in strains of Streptococcus, Bacillus, and Staphylococcus. In streptococci, the phospho-beta-glucosidase activity depends on the antigenic group. The highest activity was found in strains of group D. In group D strains, phospho-beta-gluco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.2.434-440.1969

    authors: Schaefler S,Malamy A,Green I

    更新日期:1969-08-01 00:00:00

  • Suppressor analysis reveals a role for SecY in the SecA2-dependent protein export pathway of Mycobacteria.

    abstract::All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00630-13

    authors: Ligon LS,Rigel NW,Romanchuk A,Jones CD,Braunstein M

    更新日期:2013-10-01 00:00:00

  • Mutations partially inactivating the lactose repressor of Escherichia coli.

    abstract::After treatment with N-methyl-N'-nitro-N-nitrosoguanidine, 133 independent mutants of a haploid strain of Escherichia coli able to use phenyl-beta-galactoside as a carbon source were obtained. The galactoside was specific in selecting for mutants with increases in their uninduced levels of beta-galactosidase. Virtuall...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.500-507.1974

    authors: Shineberg B

    更新日期:1974-08-01 00:00:00

  • Possible role of lectins in mycoparasitism.

    abstract::Lectin activity in a host-mycoparasite relationship was demonstrated with Rhizoctonia solani and Trichoderma harzianum. Attachment of O but not A and B erythrocytes to hyphae occurred on R. solani but not on its mycoparasite. This phenomenon, which was Ca2+ and Mn2+ dependent, was prevented by galactose, present in T....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.3.1431-1435.1983

    authors: Elad Y,Barak R,Chet I

    更新日期:1983-06-01 00:00:00

  • Genomic insights into the convergence and pathogenicity factors of Campylobacter jejuni and Campylobacter coli species.

    abstract::Whether or not bacteria form coherent evolutionary groups via means of genetic exchange and, hence, elicit distinct species boundaries remains an unsettled issue. A recent report implied that not only may the former be true but also, in fact, the clearly distinct Campylobacter jejuni and Campylobacter coli species may...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00519-09

    authors: Caro-Quintero A,Rodriguez-Castaño GP,Konstantinidis KT

    更新日期:2009-09-01 00:00:00

  • Gut Microbiota: the Emerging Link to Lung Homeostasis and Disease.

    abstract::The gut microbiota plays a crucial role in the development of the immune system and confers benefits or disease susceptibility to the host. Emerging studies have indicated the gut microbiota could affect pulmonary health and disease through cross talk between the gut microbiota and the lungs. Gut microbiota dysbiosis c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00454-20

    authors: Zhou A,Lei Y,Tang L,Hu S,Yang M,Wu L,Yang S,Tang B

    更新日期:2021-01-25 00:00:00

  • Genetic evidence for a repressor of synthesis of cytosine deaminase and purine biosynthesis enzymes in Escherichia coli.

    abstract::Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.2124-2127.1989

    authors: Kilstrup M,Meng LM,Neuhard J,Nygaard P

    更新日期:1989-04-01 00:00:00

  • Sporulation in D-glucosamine auxotrophs of Saccharomyces cerevisiae: meiosis with defective ascospore wall formation.

    abstract::Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1545-1557.1975

    authors: Whelan WL,Ballou CE

    更新日期:1975-12-01 00:00:00

  • Purification of the Escherichia coli purine regulon repressor and identification of corepressors.

    abstract::The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was required for binding o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5637-5642.1990

    authors: Rolfes RJ,Zalkin H

    更新日期:1990-10-01 00:00:00

  • Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima.

    abstract::Phosphate acetyltransferase (PTA) and acetate kinase (AK) of the hyperthermophilic eubacterium Thermotoga maritima have been purified 1,500- and 250-fold, respectively, to apparent homogeneity. PTA had an apparent molecular mass of 170 kDa and was composed of one subunit with a molecular mass of 34 kDa, suggesting a h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.6.1861-1867.1999

    authors: Bock AK,Glasemacher J,Schmidt R,Schönheit P

    更新日期:1999-03-01 00:00:00

  • Thermal denaturation of mesophilic and thermophilic 5S ribonucleic acids.

    abstract::The Tm of Bacillus stearothermophilus 5S ribonucleic acid (RNA) is 1.5 +/- 0.5 C higher than that of 5S RNAs from B. subtilis and Escherichia coli. Melting in 50% methanol and in formaldehyde indicate that both base stacking and helical regions are involved in the slightly increased thermal stability of B. stearotherm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.850-854.1976

    authors: Varricchio F,Marotta CA

    更新日期:1976-03-01 00:00:00

  • TWO LYTIC FACTORS INVOLVED IN INDUCED SYNERGISTIC HEMOLYSIS IN NORMALLY NONHEMOLYTIC STAPHYLOCOCCI.

    abstract::Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.6.1700-1704.1964

    authors: SMITH DC,FOLTZ VD,LORD TH

    更新日期:1964-12-01 00:00:00

  • A novel deaminase involved in chloronitrobenzene and nitrobenzene degradation with Comamonas sp. strain CNB-1.

    abstract::Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01762-06

    authors: Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

    更新日期:2007-04-01 00:00:00

  • Draft genome sequence of Clostridium sporogenes PA 3679, the common nontoxigenic surrogate for proteolytic Clostridium botulinum.

    abstract::Clostridium sporogenes PA 3679 is widely used as a nontoxigenic surrogate for proteolytic strains of Clostridium botulinum in the derivation and validation of thermal processes in food. Here we report the draft assembly and annotation of the C. sporogenes PA 3679 genome. Preliminary analysis demonstrates a high degree...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06765-11

    authors: Bradbury M,Greenfield P,Midgley D,Li D,Tran-Dinh N,Vriesekoop F,Brown JL

    更新日期:2012-03-01 00:00:00

  • Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1.

    abstract::The genes coding for two structurally different isocitrate dehydrogenase isozymes (IDH-I and IDH-II) of a psychrophilic bacterium, Vibrio sp. strain ABE-1, were cloned and sequenced. Open reading frames of the genes (icdI and icdII) are 1,248 and 2,229 bp in length, respectively. The amino acid sequences predicted fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.21.6873-6880.1993

    authors: Ishii A,Suzuki M,Sahara T,Takada Y,Sasaki S,Fukunaga N

    更新日期:1993-11-01 00:00:00

  • Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase.

    abstract::Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.10.2583-2589.1998

    authors: Katayama Y,Matsushita Y,Kaneko M,Kondo M,Mizuno T,Nyunoya H

    更新日期:1998-05-01 00:00:00

  • Conjugative transfer of the naturally occurring plasmids of Acetobacter xylinum by IncP-plasmid-mediated mobilization.

    abstract::Broad-host-range plasmids and cloning vectors were conjugatively transferred to Acetobacter xylinum. One of the plasmids, RP4::Mu cts61, was used for the insertion of Tn1 into the 16-, 44-, and 64-kilobase-pair plasmids of A. xylinum. The Tn1-labeled plasmids could be mobilized by a helper plasmid. Many of the Tn1 ins...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.1.336-339.1986

    authors: Valla S,Coucheron DH,Kjosbakken J

    更新日期:1986-01-01 00:00:00

  • Incompatibility in Podospora anserina: comparative properties of the antagonistic cytoplasmic factors of a nonallelic system.

    abstract::In the fungus Podospora anserina, the interaction between the nonallelic incompatible R and V genes has two consequences: a lytic reaction due to the synthesis of specific proteolytic enzymes, and a quenching in protein and ribonucleic acid synthesis. The incompatibility reaction when vegetative or sexual R and V cell...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.854-860.1974

    authors: Labarére J,Bègueret J,Bernet J

    更新日期:1974-11-01 00:00:00

  • Replication Rapidly Recovers and Continues in the Presence of Hydroxyurea in Escherichia coli.

    abstract::In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00713-17

    authors: Nazaretyan SA,Savic N,Sadek M,Hackert BJ,Courcelle J,Courcelle CT

    更新日期:2018-02-23 00:00:00

  • Implication of the VirD4 coupling protein of the Lvh type 4 secretion system in virulence phenotypes of Legionella pneumophila.

    abstract::The genome of the Philadelphia-1 strain of Legionella pneumophila, the causative organism of Legionnaires' disease, encodes two virulence-associated type 4 secretion systems (T4SSs), the Dot/Icm type 4B (T4BSS) and the Lvh type 4A (T4ASS). Broth stationary-phase cultures of most dot/icm mutants are defective in entry ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00430-13

    authors: Bandyopadhyay P,Lang EA,Rasaputra KS,Steinman HM

    更新日期:2013-08-01 00:00:00