Construction of a tyrP-lac operon fusion strain and its use in the isolation and analysis of mutants derepressed for tyrP expression.

Abstract:

:The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were transferred onto a plasmid vector by recombination in vivo. Restriction enzyme analysis of the resultant plasmids suggests that some of these mutants arose from either an insertion or a deletion of DNA occurring within the region of DNA that contains the tyrP promoter.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kasian PA,Pittard J

doi

10.1128/JB.160.1.175-183.1984

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

175-83

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

160

pub_type

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