Cell wall turnover in batch and chemostat cultures of Bacillus subtilis.

abstract：:Hoffman, Heiner (New York University, New York, N.Y.), Jon Valdina, and Michael E. Frank. Effects of high incubation temperature upon the cell wall of Escherichia coli. J. Bacteriol. 91:1635-1637. 1966.-Escherichia coli cells grown at 45 C were found to swell when suspended in distilled water, and were broken down whe...

journal_title：Journal of bacteriology

authors： Hoffman H,Valdina J,Frank ME

更新日期：1966-04-01 00:00:00

abstract：:Bacteria use three distinct systems for iron-sulfur (Fe/S) cluster biogenesis: the ISC, SUF, and NIF machineries. The ISC and SUF systems are widely distributed, and many bacteria possess both of them. In Escherichia coli, ISC is the major and constitutive system, whereas SUF is induced under iron starvation and/or ox...

journal_title：Journal of bacteriology

authors： Albrecht AG,Netz DJ,Miethke M,Pierik AJ,Burghaus O,Peuckert F,Lill R,Marahiel MA

更新日期：2010-03-01 00:00:00

abstract：:The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetracycline resistance we...

journal_title：Journal of bacteriology

authors： McNicholas P,Chopra I,Rothstein DM

更新日期：1992-12-01 00:00:00

abstract：:Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...

journal_title：Journal of bacteriology

authors： Dubourdieu M,DeMoss JA

更新日期：1992-02-01 00:00:00

abstract：:Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complemen...

journal_title：Journal of bacteriology

authors： Jeng YH,Doi RH

更新日期：1974-08-01 00:00:00

abstract：:A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...

journal_title：Journal of bacteriology

authors： Maniloff J,Quinlan DC

更新日期：1974-10-01 00:00:00

abstract：:A number of mutations (45) expressed as cold-sensitive conditional lethal pheno-types were screened by transduction for their linkage to the streptomycin-resistance locus; 7 showed such linkage. Of these, two were studied in greater detail. The sedimentation profiles of ribosomes from cultures grown at low temperature...

journal_title：Journal of bacteriology

authors： Tai PC,Kessler DP,Ingraham J

更新日期：1969-03-01 00:00:00

abstract：:Clinical isolates of the fungal respiratory and systemic pathogen Histoplasma capsulatum have been placed in several different classes by using genomic restriction fragment length polymorphisms (RFLPs), but in general have not been distinguished further. We report here that a polymerase chain reaction (PCR)-based DNA ...

journal_title：Journal of bacteriology

authors： Kersulyte D,Woods JP,Keath EJ,Goldman WE,Berg DE

更新日期：1992-11-01 00:00:00

abstract：:Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...

journal_title：Journal of bacteriology

authors： Jürgens UJ,Weckesser J

更新日期：1985-10-01 00:00:00

abstract：:Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Fur...

journal_title：Journal of bacteriology

authors： Kusuya Y,Kurokawa K,Ishikawa S,Ogasawara N,Oshima T

更新日期：2011-06-01 00:00:00

abstract：:The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

journal_title：Journal of bacteriology

authors： de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

更新日期：1989-12-01 00:00:00

abstract：:A draft genome sequence of Tsukamurella sp., an aerobic bacterium isolated from a human sputum specimen, is described here. A new virus or provirus, TPA4, was characterized. ...

journal_title：Journal of bacteriology

authors： Oulmi L,Gorlas A,Gimenez G,Robert C,Boulahrouf A,Raoult D,Roux V

更新日期：2012-10-01 00:00:00

abstract：:A 22-kb segment of chromosomal DNA from Enterococcus faecalis OG1RF containing the pyrimidine biosynthesis genes pyrC and pyrD was previously detected as complementing Escherichia coli pyrC and pyrD mutations. In the present study, it was found that the E. faecalis pyrimidine biosynthetic genes in this clone (designat...

journal_title：Journal of bacteriology

authors： Li X,Weinstock GM,Murray BE

更新日期：1995-12-01 00:00:00

abstract：:The cytochrome c(3) of Desulfovibrio desulfuricans and that of D. vulgaris were purified to homogeneity as judged by disc gel electrophoresis and by ultracentrifugation. Both cytochromes had an oxidation-reduction potential of -205 +/- 5 mv at pH 7.0 and showed characteristic absorption bands at 525 and 553 nm in the ...

journal_title：Journal of bacteriology

authors： Drucker H,Campbell LL

更新日期：1969-10-01 00:00:00

abstract：:An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...

journal_title：Journal of bacteriology

authors： Frazier BA,Pfeifer JD,Russell DG,Falk P,Olsén AN,Hammar M,Westblom TU,Normark SJ

更新日期：1993-02-01 00:00:00

abstract：:The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

journal_title：Journal of bacteriology

authors： Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

更新日期：1989-05-01 00:00:00

abstract：:D-Ribulose 1,5-bisphosphate (RuBP) carboxylase has been purified from the photosynthetic extreme halophile Ectothiorhodospira halophila. Despite a growth requirement for almost saturating sodium chloride in the medium, both crude and homogeneous preparations of RuBP carboxylase obtained from this organism were inhibit...

journal_title：Journal of bacteriology

authors： Tabita FR,McFadden BA

更新日期：1976-06-01 00:00:00

abstract：:In a previous search for mutants of Salmonella typhimurium that are defective in heme synthesis, one class that is apparently defective in 5-aminolevulinic acid (ALA) uptake (alu) was found. Here, I describe the characterization of these mutations. The mutations all map to a single locus near 77.5 min on the genetic m...

journal_title：Journal of bacteriology

authors： Elliott T

更新日期：1993-01-01 00:00:00

abstract：:Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...

journal_title：Journal of bacteriology

authors： Wilson CR,Baldwin NJ

更新日期：1978-10-01 00:00:00

abstract：:Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...

journal_title：Journal of bacteriology

authors： Cho K,Fuqua C,Martin BS,Winans SC

更新日期：1996-04-01 00:00:00

abstract：:Peptidoglycan hydrolase, LytF (CwlE), was determined to be identical to YhdD (deduced cell wall binding protein) by zymography after insertional inactivation of the yhdD gene. YhdD exhibits high sequence similarity with CwlF (PapQ, LytE) and p60 of Listeria monocytogenes. The N-terminal region of YhdD has a signal seq...

journal_title：Journal of bacteriology

authors： Ohnishi R,Ishikawa S,Sekiguchi J

更新日期：1999-05-01 00:00:00

abstract：:The mutualistic symbiont Vibrio fischeri builds a symbiotic biofilm during colonization of squid hosts. Regulation of the exopolysaccharide component, termed Syp, has been examined in strain ES114, where production is controlled by a phosphorelay that includes the inner membrane hybrid histidine kinase RscS. Most stra...

journal_title：Journal of bacteriology

authors： Rotman ER,Bultman KM,Brooks JF 2nd,Gyllborg MC,Burgos HL,Wollenberg MS,Mandel MJ

更新日期：2019-04-09 00:00:00

abstract：:The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 4.1.1.1) was deleted from the Saccharomyces cerevisiae genome. The resulting pdc1(0) mutants were able to grow on glucose and still contained 60 to 70% of the wild-type PDC activity. Two DNA fragments with sequences homologous to that of the PDC1 gene were clo...

journal_title：Journal of bacteriology

authors： Seeboth PG,Bohnsack K,Hollenberg CP

更新日期：1990-02-01 00:00:00

abstract：:Exposure of Escherichia coli strains deficient in molybdopterin biosynthesis (moa) to the purine base N-6-hydroxylaminopurine (HAP) is mutagenic and toxic. We show that moa mutants exposed to HAP also exhibit elevated mutagenesis, a hyperrecombination phenotype, and increased SOS induction. The E. coli rdgB gene encod...

journal_title：Journal of bacteriology

authors： Burgis NE,Brucker JJ,Cunningham RP

更新日期：2003-05-01 00:00:00

abstract：:The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increa...

journal_title：Journal of bacteriology

authors： Kajitani M,Kato A,Wada A,Inokuchi Y,Ishihama A

更新日期：1994-01-01 00:00:00

abstract：:A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...

journal_title：Journal of bacteriology

authors： Sabaty M,Kaplan S

更新日期：1996-01-01 00:00:00

abstract：:Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 l...

journal_title：Journal of bacteriology

authors： Ralph D,McClelland M,Welsh J,Baranton G,Perolat P

更新日期：1993-02-01 00:00:00

abstract：:Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...

journal_title：Journal of bacteriology

authors： Karunakaran KP,Noguchi Y,Read TD,Cherkasov A,Kwee J,Shen C,Nelson CC,Brunham RC

更新日期：2003-03-01 00:00:00

abstract：:A new osmotically inducible gene in Escherichia coli, osmY, was induced 8- to 10-fold by hyperosmotic stress and 2- to 3-fold by growth in complex medium. The osmY gene product is a periplasmic protein which migrates with an apparent molecular mass of 22 kDa on sodium dodecyl sulfate-polyacrylamide gels. A genetic fus...

journal_title：Journal of bacteriology

authors： Yim HH,Villarejo M

更新日期：1992-06-01 00:00:00

abstract：:Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. Th...

journal_title：Journal of bacteriology

authors： Paustian ML,May BJ,Cao D,Boley D,Kapur V

更新日期：2002-12-01 00:00:00