Halocin S8: a 36-amino-acid microhalocin from the haloarchaeal strain S8a.

abstract：:We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

journal_title：Journal of bacteriology

authors： Flache P,Baumeister R,Hillen W

更新日期：1992-04-01 00:00:00

abstract：:Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...

journal_title：Journal of bacteriology

authors： Kloft N,Forchhammer K

更新日期：2005-10-01 00:00:00

abstract：:The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

journal_title：Journal of bacteriology

authors： Amikam D,Benziman M

更新日期：1989-12-01 00:00:00

abstract：:DNA involved in the mobilization of broad-host-range plasmid R1162 was localized to a region of 2.7 kilobases within coordinates 3.4 to 6.1 kilobases on the R1162 map. By examining the transfer properties of plasmids containing cloned fragments of DNA from within this region, we showed that at least four trans-active ...

journal_title：Journal of bacteriology

authors： Brasch MA,Meyer RJ

更新日期：1986-08-01 00:00:00

abstract：:E2 (dihydrolipoamide acetyltransferase) and E3 (dihydrolipoamide dehydrogenase) of the Clostridium magnum acetoin dehydrogenase enzyme system were copurified in a three-step procedure from acetoin-grown cells. The denatured E2-E3 preparation comprised two polypeptides with M(r)s of 49,000 and 67,000, respectively. Mic...

journal_title：Journal of bacteriology

authors： Krüger N,Oppermann FB,Lorenzl H,Steinbüchel A

更新日期：1994-06-01 00:00:00

abstract：:The hook-basal body complex of Salmonella typhimurium, a major component of its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. The study was facilitated by the development of an improved protocol for isolation of the complexes in high yield and purity. Nine prot...

journal_title：Journal of bacteriology

authors： Aizawa SI,Dean GE,Jones CJ,Macnab RM,Yamaguchi S

更新日期：1985-03-01 00:00:00

abstract：:The bacterium Corynebacterium pseudotuberculosis is of major veterinary importance because it affects livestock, particularly sheep, goats, and horses, in several countries, including Australia, Brazil, the United States, and Canada, resulting in significant economic losses. In the present study, we describe the compl...

journal_title：Journal of bacteriology

authors： Ramos RT,Silva A,Carneiro AR,Pinto AC,Soares Sde C,Santos AR,Almeida SS,Guimarães LC,Aburjaile FF,Barbosa EG,Dorella FA,Rocha FS,Cerdeira LT,Barbosa MS,Tauch A,Edman J,Spier S,Miyoshi A,Schneider MP,Azevedo V

更新日期：2012-12-01 00:00:00

abstract：:Lipoprotein biogenesis in Gram-negative bacteria occurs by a conserved pathway, each step of which is considered essential. In contrast to this model, LoVullo and colleagues demonstrate that the N-acyl transferase Lnt is not required in Francisella tularensis or Neisseria gonorrhoeae. This suggests the existence of a ...

journal_title：Journal of bacteriology

authors： Chahales P,Thanassi DG

更新日期：2015-05-01 00:00:00

abstract：:Replication initiation depends on origin recognition, helicase, and primase activities. In phage P4, a second DNA region, the cis replication region (crr), is also required for replication initiation. The multifunctional alpha protein of phage P4, which is essential for DNA replication, combines the three aforemention...

journal_title：Journal of bacteriology

authors： Ziegelin G,Calendar R,Lurz R,Lanka E

更新日期：1997-07-01 00:00:00

abstract：:Studies by R. Lin et al. (J. Bacteriol. 174:1948-1955, 1992) suggested that the Escherichia coli leu operon might be a member of the Lrp regulon. Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lacking leucine, there was little difference in leu operon expression between ...

journal_title：Journal of bacteriology

authors： Landgraf JR,Boxer JA,Calvo JM

更新日期：1999-10-01 00:00:00

abstract：:The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...

journal_title：Journal of bacteriology

authors： Kang L,Keeler ML,Dunlop PC,Roon RJ

更新日期：1982-07-01 00:00:00

abstract：:The highly purified lipoprotein of the outer membrane of Escherichia coli forms paracrystals. The ultrastructures of these paracrystals were examined by electron microscopy. The needle-shaped paracrystals show several different band patterns, depending on conditions of paracrystallization. Models are presented to expl...

journal_title：Journal of bacteriology

authors： DeMartini M,Inouye S,Inouye M

更新日期：1976-07-01 00:00:00

abstract：:Two isozymes of chorismate mutase (CA mutase(1) and CA mutase(2)) and two isozymes of prephenate dehydratase (PPA dehydratase(1) and PPA dehydratase(2)) have been found in Pseudomonas aeruginosa. The activities CA mutase(2)-PPA dehydratase(2) catalyzing phenylalanine biosynthesis have been purified almost 40-fold and ...

journal_title：Journal of bacteriology

authors： Ahmed SI,Campbell JJ

更新日期：1973-07-01 00:00:00

abstract：:Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

journal_title：Journal of bacteriology

authors： Kosugi A,Murashima K,Doi RH

更新日期：2001-12-01 00:00:00

abstract：:Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that th...

journal_title：Journal of bacteriology

authors： MacPhee KG,Nelson RE,Schuster SM

更新日期：1983-10-01 00:00:00

abstract：:Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...

journal_title：Journal of bacteriology

authors： Mokrousov I,Vyazovaya A,Narvskaya O

更新日期：2014-05-01 00:00:00

abstract：:The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To...

journal_title：Journal of bacteriology

authors： Takeuchi K,Taguchi F,Inagaki Y,Toyoda K,Shiraishi T,Ichinose Y

更新日期：2003-11-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...

journal_title：Journal of bacteriology

authors： Chang AK,Kim HY,Park JE,Acharya P,Park IS,Yoon SM,You HJ,Hahm KS,Park JK,Lee JS

更新日期：2005-10-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide...

journal_title：Journal of bacteriology

authors： Lee CA,Grossman AD

更新日期：2007-10-01 00:00:00

abstract：:Most bacteria are likely to face osmotic challenges, but there is yet much to learn about how such environmental changes affect the architecture of bacterial cells. Here, we report a cell-biological study in model organisms of the genus Streptomyces, which are actinobacteria that grow in a highly polarized fashion to ...

journal_title：Journal of bacteriology

authors： Fuchino K,Flärdh K,Dyson P,Ausmees N

更新日期：2016-12-13 00:00:00

abstract：:An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K. ...

journal_title：Journal of bacteriology

authors： Matney TS,MacDougall NL,Butler MA,Suit JC

更新日期：1970-10-01 00:00:00

abstract：:The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...

journal_title：Journal of bacteriology

authors： Hommes NG,Sayavedra-Soto LA,Arp DJ

更新日期：2001-02-01 00:00:00

abstract：:Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

journal_title：Journal of bacteriology

authors： Joenje H,Konings WN,Venema G

更新日期：1974-09-01 00:00:00

abstract：:The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

journal_title：Journal of bacteriology

authors： Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

更新日期：2005-04-01 00:00:00

abstract：:Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...

journal_title：Journal of bacteriology

authors： Martinez RJ,Gordee EZ

更新日期：1966-02-01 00:00:00

abstract：:Alysiella bovis adheres to surfaces by means of short, ruthenium red-staining, rod-like fimbriae. The fimbriae remain associated with the cell envelope of A. bovis, even when sonicated or exposed sequentially to toluene, Triton X-100, lysozyme, ribonuclease, and deoxyribonuclease. Adhesion of outer membrane-derived ce...

journal_title：Journal of bacteriology

authors： Irvin RT,To M,Costerton JW

更新日期：1984-11-01 00:00:00

abstract：:CluA is a cell surface-presented protein that causes cell aggregation and is essential for a high-efficiency conjugation process in Lactococcus lactis. We know from previous work that in addition to promoting cell-to-cell contact, CluA is involved in sex factor DNA transfer. To define the CluA domains involved in aggr...

journal_title：Journal of bacteriology

authors： Stentz R,Gasson M,Shearman C

更新日期：2006-03-01 00:00:00

abstract：:Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...

journal_title：Journal of bacteriology

authors： Krebs MP,Khorana HG

更新日期：1993-03-01 00:00:00

abstract：:The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequence affording iron regulation of a iucA'-'lacZ gene fusion. A 78-base-pair (bp) region containing the main (P1) promoter retained...

journal_title：Journal of bacteriology

authors： de Lorenzo V,Wee S,Herrero M,Neilands JB

更新日期：1987-06-01 00:00:00