A HilA-independent pathway to Salmonella typhimurium invasion gene transcription.

Abstract:

:Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outside (SirA) SPI1. Two unique loci, sirB and sirC, which contribute to SPI1 gene transcription were defined. sirC is an SPI1-encoded transcription factor of the AraC family that contributes to the invasive phenotype. sirB is required for maximal expression of sirC and consists of two open reading frames located near kdsA, a gene involved in lipopolysaccharide biosynthesis. sirC expression, unlike expression of other SPI1 genes, does not require HilA. Overexpression of sirC or sirA restores expression of a subset of SPI1 genes, including invF and sspC, in the absence of HilA. These data define roles for SirC and SirA as part of a HilA-independent pathway to SPI1 gene expression. We postulate that HilA-independent activation of inv expression is important for efficient assembly and function of the SPI1 TTSS.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rakeman JL,Bonifield HR,Miller SI

doi

10.1128/JB.181.10.3096-3104.1999

keywords:

subject

Has Abstract

pub_date

1999-05-01 00:00:00

pages

3096-104

issue

10

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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