Overproduction of tryptophanyl-tRNA synthetase relieves transcription termination at the Escherichia coli tryptophan operon attenuator.

abstract：:The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

journal_title：Journal of bacteriology

authors： Reniere ML

更新日期：2018-08-10 00:00:00

abstract：:The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs block...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ

更新日期：1974-06-01 00:00:00

abstract：:Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...

journal_title：Journal of bacteriology

authors： Wang J,Hofnung M,Charbit A

更新日期：2000-01-01 00:00:00

abstract：:The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...

journal_title：Journal of bacteriology

authors： Harding NE,Cleary JM,Smith DW,Michon JJ,Brusilow WS,Zyskind JW

更新日期：1982-12-01 00:00:00

abstract：:Two related killer strains of Saccharomyces cerevisiae were mutagenized and screened for nonkiller variants. About 20% of the mutants derived from one strain lacked all detectable double-straned ribonucleic acid (dsRNA). About 70% of the mutants from the other strain lacked one of the dsRNA species normally associated...

journal_title：Journal of bacteriology

authors： Vodkin M

更新日期：1977-10-01 00:00:00

abstract：:Acetyl coenzyme A synthetase (Acs) activates acetate to acetyl coenzyme A through an acetyladenylate intermediate; two other enzymes, acetate kinase (Ack) and phosphotransacetylase (Pta), activate acetate through an acetyl phosphate intermediate. We subcloned acs, the Escherichia coli open reading frame purported to e...

journal_title：Journal of bacteriology

authors： Kumari S,Tishel R,Eisenbach M,Wolfe AJ

更新日期：1995-05-01 00:00:00

abstract：:Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...

journal_title：Journal of bacteriology

authors： Skurray RA,Reeves P

更新日期：1974-01-01 00:00:00

abstract：:The uptake of iron into Pseudomonas aeruginosa is mediated by two major siderophores produced by the bacterium, pyoverdine and pyochelin. The bacterium is also able of utilize several heterologous siderophores of bacterial or fungal origin. In this work, we have investigated the iron uptake in P. aeruginosa PAO1 by th...

journal_title：Journal of bacteriology

authors： Hannauer M,Barda Y,Mislin GL,Shanzer A,Schalk IJ

更新日期：2010-03-01 00:00:00

abstract：:An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...

journal_title：Journal of bacteriology

authors： Ma Z,Richard H,Tucker DL,Conway T,Foster JW

更新日期：2002-12-01 00:00:00

abstract：:Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...

journal_title：Journal of bacteriology

authors： LYONS AJ Jr,PRIDHAM TG

更新日期：1962-02-01 00:00:00

abstract：:In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

journal_title：Journal of bacteriology

authors： Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

更新日期：2017-05-09 00:00:00

abstract：:We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...

journal_title：Journal of bacteriology

authors： Stults LW,Moshiri F,Maier RJ

更新日期：1986-06-01 00:00:00

abstract：:Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its ac...

journal_title：Journal of bacteriology

authors： Ramírez S,Moreno R,Zafra O,Castán P,Vallés C,Berenguer J

更新日期：2000-04-01 00:00:00

abstract：:The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion protei...

journal_title：Journal of bacteriology

authors： Yakushi T,Hattori N,Homma M

更新日期：2005-01-01 00:00:00

abstract：:To isolate genes from Escherichia coli which regulate the labile hydrogenase activity, a plasmid library was used to transform hydL mutants lacking the labile hydrogenase. A single type of gene, designated hydG, was isolated. This gene also partially restored the hydrogenase activity in hydF mutants (which are defecti...

journal_title：Journal of bacteriology

authors： Stoker K,Reijnders WN,Oltmann LF,Stouthamer AH

更新日期：1989-08-01 00:00:00

abstract：:During spore formation, Bacillus subtilis divides asymmetrically, resulting in two cells with different fates. Immediately after division, the transcription factor σ(F) becomes active in the smaller prespore, followed by activation of σ(E) in the larger mother cell. We recently showed that a delay in σ(E) activation r...

journal_title：Journal of bacteriology

authors： Xenopoulos P,Piggot PJ

更新日期：2011-06-01 00:00:00

abstract：:Attaching and effacing (AE) pathogens colonize the gut mucosa using a type three secretion system (T3SS) and a suite of effector proteins. The locus of enterocyte effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive...

journal_title：Journal of bacteriology

authors： Furniss RCD,Clements A

更新日期：2017-12-20 00:00:00

abstract：:Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

journal_title：Journal of bacteriology

authors： Bingle WH,Nomellini JF,Smit J

更新日期：1997-02-01 00:00:00

abstract：:Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridi...

journal_title：Journal of bacteriology

authors： Kimura A,Hirose K,Kariya Y,Nagai S

更新日期：1976-02-01 00:00:00

abstract：:There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1985-12-01 00:00:00

abstract：:Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...

journal_title：Journal of bacteriology

authors： Madduri K,Stuttard C,Vining LC

更新日期：1989-01-01 00:00:00

abstract：:Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

journal_title：Journal of bacteriology

authors： Short KA,Blakemore RP

更新日期：1986-08-01 00:00:00

abstract：:Proteus mirabilis, an agent of urinary tract infection, expresses at least four fimbrial types. Among these are the MR/P (mannose-resistant/Proteus-like) fimbriae. MrpA, the structural subunit, is optimally expressed at 37 degrees C in Luria broth cultured statically for 48 h by each of seven strains examined. Genes e...

journal_title：Journal of bacteriology

authors： Bahrani FK,Mobley HL

更新日期：1994-06-01 00:00:00

abstract：:We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The protein products of TraU+ clones were identified, and the nucleotide sequence of traU was determined. traU mapped betwee...

journal_title：Journal of bacteriology

authors： Moore D,Maneewannakul K,Maneewannakul S,Wu JH,Ippen-Ihler K,Bradley DE

更新日期：1990-08-01 00:00:00

abstract：:Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...

journal_title：Journal of bacteriology

authors： ISHIBASHI M,SUGINO Y,HIROTA Y

更新日期：1964-03-01 00:00:00

abstract：:To investigate the involvement of bacterial catalases of the symbiotic gram-negative bacterium Rhizobium meliloti in the development of Medicago-Rhizobium functional nodules, we cloned a putative kat gene by screening a cosmid library with a catalase-specific DNA probe amplified by PCR from the R. meliloti genome. Nuc...

journal_title：Journal of bacteriology

authors： Hérouart D,Sigaud S,Moreau S,Frendo P,Touati D,Puppo A

更新日期：1996-12-01 00:00:00

abstract：:The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same cu...

journal_title：Journal of bacteriology

authors： Hiraga S,Jaffé A,Ogura T,Mori H,Takahashi H

更新日期：1986-04-01 00:00:00

abstract：:Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...

journal_title：Journal of bacteriology

authors： Cook WR,MacAlister TJ,Rothfield LI

更新日期：1986-12-01 00:00:00

abstract：:Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...

journal_title：Journal of bacteriology

authors： Rismondo J,Schulz LM,Yacoub M,Wadhawan A,Hoppert M,Dionne MS,Gründling A

更新日期：2021-01-25 00:00:00

abstract：:To examine the role of the amino acid residues (between positions 258 and 275 and positions 297 and 298) of the alpha-subunit of RNA polymerase in TyrR-mediated activation of the mtr promoter, we have carried out in vitro transcription experiments using a set of mutant RNA polymerases with a supercoiled mtr template. ...

journal_title：Journal of bacteriology

authors： Yang J,Murakami K,Camakaris H,Fujita N,Ishihama A,Pittard AJ

更新日期：1997-10-01 00:00:00