Purification and characterization of NADP(+)-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Peptostreptococcus productus marburg.

abstract：:The gene encoding the major NAD(P)H-flavin oxidoreductase (flavin reductase) of the luminous bacterium Vibrio fischeri ATCC 7744 was isolated by using synthetic oligonucleotide probes corresponding to the N-terminal amino acid sequence of the enzyme. Nucleotide sequence analysis suggested that the major flavin reducta...

journal_title：Journal of bacteriology

authors： Zenno S,Saigo K,Kanoh H,Inouye S

更新日期：1994-06-01 00:00:00

abstract：:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

journal_title：Journal of bacteriology

authors： Livingston DM,Klein HL

更新日期：1977-01-01 00:00:00

abstract：:In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

journal_title：Journal of bacteriology

authors： Matsuyama T,Bhasin A,Harshey RM

更新日期：1995-02-01 00:00:00

abstract：:Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...

journal_title：Journal of bacteriology

authors： Liu L,Wu JF,Ma YF,Wang SY,Zhao GP,Liu SJ

更新日期：2007-04-01 00:00:00

abstract：:Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...

journal_title：Journal of bacteriology

authors： Kawamoto I,Oka T,Nara T

更新日期：1981-05-01 00:00:00

abstract：:The E. coli RelA protein is a ribosome-dependent (p)ppGpp synthetase that is activated in response to amino acid starvation. RelA can be dissected both functionally and physically into two domains: The N-terminal domain (NTD) (amino acids [aa] 1 to 455) contains the catalytic domain of RelA, and the C-terminal domain ...

journal_title：Journal of bacteriology

authors： Gropp M,Strausz Y,Gross M,Glaser G

更新日期：2001-01-01 00:00:00

abstract：:Transcriptional expression of the puc operon in Rhodobacter sphaeroides 2.4.1 is dependent on the partial pressure of oxygen. By using transcriptional fusions in trans of a promoterless fragment derived from the aminoglycoside-3'-phosphotransferase gene of Tn903 to puc operon-specific DNA containing a 629-bp 5' cis-ac...

journal_title：Journal of bacteriology

authors： Lee JK,Kaplan S

更新日期：1992-02-01 00:00:00

abstract：:Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...

journal_title：Journal of bacteriology

authors： Golub EE,Bronner F

更新日期：1974-09-01 00:00:00

abstract：:Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group of low-copy-number plasmids in gram-positive bacteria that replicate via a theta mechanism of replication. We studied the DNA region encoding the replication protein, RepN, of pLS32, and obtained the following results. Transcription of the repN...

journal_title：Journal of bacteriology

authors： Tanaka T,Ishida H,Maehara T

更新日期：2005-07-01 00:00:00

abstract：:Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...

journal_title：Journal of bacteriology

authors： Daumy GO,McColl AS,Andrews GC

更新日期：1980-01-01 00:00:00

abstract：:The structural gene for the F sex factor outer membrane surface exclusion protein ( traT ) was cloned onto a high-copy-number plasmid where it is expressed from the phage lambda promoter pL. Conditional control over expression was provided by a temperature-sensitive lambda cI repressor. Induction of pL produced large ...

journal_title：Journal of bacteriology

authors： Minkley EG Jr

更新日期：1984-05-01 00:00:00

abstract：:Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...

journal_title：Journal of bacteriology

authors： Schrader JA,Holmes DS

更新日期：1988-09-01 00:00:00

abstract：:Synthesis of formate dehydrogenase coupled to formate hydrogenlyase activity in Escherichia coli was found to require the product of the fhlA gene. Transcription of fdhF, the gene coding for the 80-kilodalton (kDa) selenopeptide of formate dehydrogenase, was not detected in an fhlA genetic background. Mutations in the...

journal_title：Journal of bacteriology

authors： Sankar P,Lee JH,Shanmugam KT

更新日期：1988-12-01 00:00:00

abstract：:Caldicellulosiruptor bescii efficiently degrades cellulose, xylan, and native grasses at high temperatures above 70°C under anaerobic conditions. C. bescii extracellularly secretes multidomain glycoside hydrolases along with proteins of unknown function. In this study, we analyzed the C. bescii proteins that bind to t...

journal_title：Journal of bacteriology

authors： Yokoyama H,Yamashita T,Morioka R,Ohmori H

更新日期：2014-11-01 00:00:00

abstract：:Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown...

journal_title：Journal of bacteriology

authors： Ball WJ Jr,Atkinson DE

更新日期：1975-03-01 00:00:00

abstract：:A superfamily of transport proteins, which includes the lactose permease of Escherichia coli, contains a highly conserved motif, G-X-X-X-D/E-R/K-X-G-R/K-R/K, in the loops that connect transmembrane segments 2 and 3 and transmembrane segments 8 and 9. Previous analysis of this motif in the lactose permease (A. E. Jesse...

journal_title：Journal of bacteriology

authors： Jessen-Marshall AE,Parker NJ,Brooker RJ

更新日期：1997-04-01 00:00:00

abstract：:tolC mutants, which are resistant to colicin E1 and also highly sensitive to detergents and dyes, were shown to lack the OmpF outer membrane protein. There was little effect on transcription as judged by the use of an ompF-lac operon fusion strain, and the tolC effect was probably due to a post-transcriptional effect....

journal_title：Journal of bacteriology

authors： Morona R,Reeves P

更新日期：1982-06-01 00:00:00

abstract：:A systematic survey for the presence of plasmids in 17 different xenobiotic-degrading Sphingomonas strains was performed. In almost all analyzed strains, two to five plasmids with sizes of about 50 to 500 kb were detected by using pulsed-field gel electrophoresis. A comparison of plasmid preparations untreated or trea...

journal_title：Journal of bacteriology

authors： Basta T,Keck A,Klein J,Stolz A

更新日期：2004-06-01 00:00:00

abstract：:The virulence of Staphylococcus aureus is controlled by the accessory gene regulator (agr) system, including an extracellular inducer encoded by agrD. Variable agr PCR restriction fragment length polymorphism (RFLP) patterns of unique S. aureus strains (n = 192) were determined for a region comprising agrD and parts o...

journal_title：Journal of bacteriology

authors： van Leeuwen W,van Nieuwenhuizen W,Gijzen C,Verbrugh H,van Belkum A

更新日期：2000-10-01 00:00:00

abstract：UNLABELLED:Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for thymineless death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the...

journal_title：Journal of bacteriology

authors： Itsko M,Schaaper RM

更新日期：2016-05-13 00:00:00

abstract：:Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complemen...

journal_title：Journal of bacteriology

authors： Jeng YH,Doi RH

更新日期：1974-08-01 00:00:00

abstract：:The O-repeating unit of the Escherichia coli O7-specific lipopolysaccharide is made of galactose, mannose, rhamnose, 4-acetamido-4,6-dideoxyglucose, and N-acetyglucosamine. We have recently characterized the genes involved in the biosynthesis of the sugar precursor GDP-mannose occurring in the E. coli O7:K1 strain VW1...

journal_title：Journal of bacteriology

authors： Marolda CL,Valvano MA

更新日期：1995-10-01 00:00:00

abstract：:The structural genes of ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) from Salmonella typhimurium LT2 were cloned on a 5.8-kilobase-pair insert in the SalI site of pBR322. A single strand specific radioactive probe containing the N terminus of the Escherichia coli K-12 glgC gene in M13mp8 was used t...

journal_title：Journal of bacteriology

authors： Leung PS,Preiss J

更新日期：1987-09-01 00:00:00

abstract：:The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3' ...

journal_title：Journal of bacteriology

authors： Healy FG,Krasnoff SB,Wach M,Gibson DM,Loria R

更新日期：2002-04-01 00:00:00

abstract：:Agrobacterium tumefaciens and Rhizobium meliloti carry related genetic loci which have important roles in virulence and symbiosis. Previously, it was shown that two virulence loci of A. tumefaciens, chvA and chvB, are related to two R. meliloti symbiosis loci, ndvA and ndvB, respectively (T. Dylan, L. Ielpi, S. Stanfi...

journal_title：Journal of bacteriology

authors： Marks JR,Lynch TJ,Karlinsey JE,Thomashow MF

更新日期：1987-12-01 00:00:00

abstract：:Colicin E2 was partially purified from Escherichia coli W3110. This preparation was remarkably stabilized by bovine serum albumin in a solution at neutral pH, as shown by dilution experiments and tests on heat stability of colicin. One killing unit of colicin E2 was estimated to correspond to one molecule of colicin E...

journal_title：Journal of bacteriology

authors： Mitsui E,Mizuno D

更新日期：1969-11-01 00:00:00

abstract：:Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...

journal_title：Journal of bacteriology

authors： Costello GM,Vipond R,MacIntyre S

更新日期：1996-03-01 00:00:00

abstract：:The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase ...

journal_title：Journal of bacteriology

authors： Curd H,Liu D,Reeves PR

更新日期：1998-02-01 00:00:00

abstract：:Poly(U)- and poly(UG)-programmed cell-free systems were developed from the extreme thermophilic, anaerobic eubacterium Thermotoga maritima, and their susceptibility to aminoglycoside and other antibiotics was assayed at a temperature (75 degrees C) close to the physiological optimum (80 degrees C) for cell growth and ...

journal_title：Journal of bacteriology

authors： Londei P,Altamura S,Huber R,Stetter KO,Cammarano P

更新日期：1988-09-01 00:00:00

abstract：:Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...

journal_title：Journal of bacteriology

authors： Cook WR,MacAlister TJ,Rothfield LI

更新日期：1986-12-01 00:00:00