The Clostridium difficile cpr locus is regulated by a noncontiguous two-component system in response to type A and B lantibiotics.

Abstract:

:The intestinal pathogen Clostridium difficile is known to grow only within the intestines of mammals, yet little is known about how the bacterium subsists in this environment. In the intestine, C. difficile must contend with innate defenses within the host, such as cationic antimicrobial peptides (CAMPs) produced by the host and the indigenous microbiota. In this study, we investigated the mechanism of activation and regulation of the CprABC transporter system, which provides resistance to multiple CAMPs and shows homology to the immunity systems of bacterial antimicrobial peptide producers. The CprABC system proved to be controlled by a noncontiguous two-component system consisting of the CprK sensor kinase and an orphan response regulator (CD3320; CprR). The CprK-CprR regulators were shown to activate cprABCK transcription in a manner similar to that by lantibiotic regulatory systems. Unlike lantibiotic producer regulation, regulation by CprK-CprR was activated by multiple lantibiotics produced by diverse Gram-positive bacteria. We identified a motif within these lantibiotics that is likely required for activation of cpr. Based on the similarities between the Cpr system and lantibiotic systems, we propose that the CprABC transporter and its regulators are relatives of lantibiotic systems that evolved to recognize multiple substrates to defend against toxins made by the intestinal microbiota.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Suárez JM,Edwards AN,McBride SM

doi

10.1128/JB.00166-13

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

2621-31

issue

11

eissn

0021-9193

issn

1098-5530

pii

JB.00166-13

journal_volume

195

pub_type

杂志文章
  • Glycosylation is required for outer membrane localization of the lectin LecB in Pseudomonas aeruginosa.

    abstract::The fucose-/mannose-specific lectin LecB from Pseudomonas aeruginosa is transported to the outer membrane; however, the mechanism used is not known so far. Here, we report that LecB is present in the periplasm of P. aeruginosa in two variants of different sizes. Both were functional and could be purified by their affi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01507-10

    authors: Bartels KM,Funken H,Knapp A,Brocker M,Bott M,Wilhelm S,Jaeger KE,Rosenau F

    更新日期:2011-03-01 00:00:00

  • Purification and characterization of the beta-galactosidase of Aeromonas formicans.

    abstract::Rohlfing, S. R. (Western Reserve University, Cleveland, Ohio), and I. P. Crawford. Purification and characterization of the beta-galactosidase of Aeromonas formicans. J. Bacteriol. 91:1085-1097. 1966.-The beta-galactosidase of Aeromonas formicans was purified by diethylaminoethyl cellulose chromatography and gel filtr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1085-1097.1966

    authors: Rohlfing SR,Crawford IP

    更新日期:1966-03-01 00:00:00

  • Dynamics of two Phosphorelays controlling cell cycle progression in Caulobacter crescentus.

    abstract::In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00992-09

    authors: Chen YE,Tsokos CG,Biondi EG,Perchuk BS,Laub MT

    更新日期:2009-12-01 00:00:00

  • The surfactant of Legionella pneumophila Is secreted in a TolC-dependent manner and is antagonistic toward other Legionella species.

    abstract::When Legionella pneumophila grows on agar plates, it secretes a surfactant that promotes flagellum- and pilus-independent "sliding" motility. We isolated three mutants that were defective for surfactant. The first two had mutations in genes predicted to encode cytoplasmic enzymes involved in lipid metabolism. These ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05405-11

    authors: Stewart CR,Burnside DM,Cianciotto NP

    更新日期:2011-11-01 00:00:00

  • Generic identification of L-forms by polyacrylamide gel electrophoretic comparison of extracts from parent strains and their derived L-forms.

    abstract::A polyacrylamide gel electrophoretic method was used for identification of L-forms in the genera Streptococcus, Staphylococcus, and Proteus, by comparing phenol-acetic acid-water extracts of homologous parent L-form pairs to one another and to other pairs. The method requires only milligram quantities of material for ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.71-77.1969

    authors: King JR,Theodore TS,Cole RM

    更新日期:1969-10-01 00:00:00

  • Replication Rapidly Recovers and Continues in the Presence of Hydroxyurea in Escherichia coli.

    abstract::In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00713-17

    authors: Nazaretyan SA,Savic N,Sadek M,Hackert BJ,Courcelle J,Courcelle CT

    更新日期:2018-02-23 00:00:00

  • Evolution of genomic content in the stepwise emergence of Escherichia coli O157:H7.

    abstract::Genome comparisons have demonstrated that dramatic genetic change often underlies the emergence of new bacterial pathogens. Evolutionary analysis of Escherichia coli O157:H7, a pathogen that has emerged as a worldwide public health threat in the past two decades, has posited that this toxin-producing pathogen evolved ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.5.1783-1791.2005

    authors: Wick LM,Qi W,Lacher DW,Whittam TS

    更新日期:2005-03-01 00:00:00

  • Differential expression of two catechol 1,2-dioxygenases in Burkholderia sp. strain TH2.

    abstract::Burkholderia sp. strain TH2, a 2-chlorobenzoate (2CB)-degrading bacterium, metabolizes benzoate (BA) and 2CB via catechol. Two different gene clusters for the catechol ortho-cleavage pathway (cat1 and cat2) were cloned from TH2 and analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.20.5714-5722.2002

    authors: Suzuki K,Ichimura A,Ogawa N,Hasebe A,Miyashita K

    更新日期:2002-10-01 00:00:00

  • Structure of the HopA1(21-102)-ShcA chaperone-effector complex of Pseudomonas syringae reveals conservation of a virulence factor binding motif from animal to plant pathogens.

    abstract::Pseudomonas syringae injects numerous bacterial proteins into host plant cells through a type 3 secretion system (T3SS). One of the first such bacterial effectors discovered, HopA1, is a protein that has unknown functions in the host cell but possesses close homologs that trigger the plant hypersensitive response in r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01621-12

    authors: Janjusevic R,Quezada CM,Small J,Stebbins CE

    更新日期:2013-02-01 00:00:00

  • Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation.

    abstract::When allyl alcohol was used as a suicide substrate, spontaneous mutants and UV light- and nitrous acid-generated mutants of Methylobacterium organophilum XX were selected which grew on methylamine but not on methanol. There was no detectable methanol dehydrogenase (MDH) activity in crude extracts of these mutants, yet...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.141-148.1988

    authors: Machlin SM,Tam PE,Bastien CA,Hanson RS

    更新日期:1988-01-01 00:00:00

  • Role of cell wall in Saccharomyces cerevisiae mutants resistant to Hg2+.

    abstract::Hg2+-resistant mutants were isolated from Saccharomyces cerevisiae. Although they were very much like the parental strains in terms of colony-forming ability, they grew faster than the parental strains in the presence of sublethal doses of Hg2+. The Hg2+-resistant mutations were dominant. They were centromere linked a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5877-5882.1988

    authors: Ono B,Ohue H,Ishihara F

    更新日期:1988-12-01 00:00:00

  • Genetic analysis of the chitinase system of Serratia marcescens 2170.

    abstract::To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7111-7117.1997

    authors: Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

    更新日期:1997-11-01 00:00:00

  • Pseudomonas cepacia mutants blocked in the Entner-Doudoroff pathway.

    abstract::Pseudomonas cepacia mutants deficient in either 6-phosphogluconate (6PGA) dehydratase (Edd-) or 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase (Eda-) failed to utilize glucose or gluconate despite the prominence of of 6-phosphogluconate dehydrogenase (6PGAD) ii this bacterium and the potential for utilizing the pen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.3.1340-1347.1982

    authors: Allenza P,Lessie TG

    更新日期:1982-06-01 00:00:00

  • General stress transcription factor sigmaB and its role in acid tolerance and virulence of Listeria monocytogenes.

    abstract::The gene encoding the general stress transcription factor sigmaB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification. Evidence for gene identification includes the following: (i) phylogenetic analyses of reported amino acid sequences for ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.14.3650-3656.1998

    authors: Wiedmann M,Arvik TJ,Hurley RJ,Boor KJ

    更新日期:1998-07-01 00:00:00

  • Complementation of a Rhodopseudomonas sphaeroides ribulose bisphosphate carboxylase-oxygenase regulatory mutant from a genomic library.

    abstract::A genomic library containing HindIII partial digests of Rhodopseudomonas sphaeroides HR DNA was constructed in the broad-host-range cosmid cloning vector pVK102. With a portion of this library as donor in complementation studies with the ribulose 1,5-bisphosphate carboxylase-oxygenase regulatory mutant R. sphaeroides ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.147-154.1985

    authors: Weaver KE,Tabita FR

    更新日期:1985-10-01 00:00:00

  • Mutations in HlyD, part of the type 1 translocator for hemolysin secretion, affect the folding of the secreted toxin.

    abstract::HlyD, a member of the membrane fusion protein family, is essential for the secretion of the RTX hemolytic toxin HlyA from Escherichia coli. Random point mutations affecting HlyA secretion were obtained, distributed in most periplasmic regions of the HlyD molecule. Analysis of the secretion phenotypes of different muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.21.7471-7480.2005

    authors: Pimenta AL,Racher K,Jamieson L,Blight MA,Holland IB

    更新日期:2005-11-01 00:00:00

  • Structural genes for flagellar hook-associated proteins in Salmonella typhimurium.

    abstract::The flaW, flaU, and flaV genes of Salmonella typhimurium LT2 were cloned into pBR322. These genes were mapped on the cloned DNA fragments by restriction endonuclease analysis and construction of the deletion derivatives. Their gene products were identified, by the minicell method, as proteins whose molecular weights w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.2.464-471.1985

    authors: Homma M,Kutsukake K,Iino T

    更新日期:1985-08-01 00:00:00

  • Choline fermentation by Desulfovibrio desulfuricans.

    abstract::Baker, F. D. (Western Reserve University, Cleveland, Ohio), H. R. Papiska, and L. Leon Campbell. Choline fermentation by Desulfovibrio desulfuricans. J. Bacteriol. 84:973-978. 1962-Hayward and Stadtman pointed out that the organism they described as Vibrio cholinicus is closely related to Desulfovibrio desulfuricans. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.5.973-978.1962

    authors: BAKER FD,PAPISKA HR,CAMPBELL LL

    更新日期:1962-11-01 00:00:00

  • Formation of Streptococcus pneumoniae non-phase-variable colony variants is due to increased mutation frequency present under biofilm growth conditions.

    abstract::In this report, we show that biofilm formation by Streptococcus pneumoniae serotype 19 gives rise to variants (the small mucoid variant [SMV] and the acapsular small-colony variant [SCV]) differing in capsule production, attachment, and biofilm formation compared to wild-type strains. All biofilm-derived variants harb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00707-08

    authors: Allegrucci M,Sauer K

    更新日期:2008-10-01 00:00:00

  • KINETICS OF THE ACTION OF AMPICILLIN ON ESCHERICHIA COLI.

    abstract::Seligman, Stephen J. (University of California, Los Angeles) and William L. Hewitt. Kinetics of the action of ampicillin on Escherichia coli. J. Bacteriol. 85:1160-1164. 1963.-The curve of the number of viable Escherichia coli after exposure to ampicillin can be divided into three phases: a lag phase, a rapid bacteric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.5.1160-1164.1963

    authors: SELIGMAN SJ,HEWITT WL

    更新日期:1963-05-01 00:00:00

  • Evolutionary and diagnostic implications of intragenomic heterogeneity in the 16S rRNA gene in Aeromonas strains.

    abstract::Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.18.6561-6564.2005

    authors: Morandi A,Zhaxybayeva O,Gogarten JP,Graf J

    更新日期:2005-09-01 00:00:00

  • Cloning, sequencing, and expression of the gene coding for bile acid 7 alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708.

    abstract::Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.15.4558-4569.1991

    authors: Baron SF,Franklund CV,Hylemon PB

    更新日期:1991-08-01 00:00:00

  • Protein export by a gram-negative bacterium: production of aerolysin by Aeromonas hydrophila.

    abstract::The synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium Aeromonas hydrophila, was studied by pulse-labeling with [35S]methionine. The toxin was synthesized as a higher-molecular-weight precursor. This was processed cotranslationally, resulting in the appearance wit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.3.1118-1124.1985

    authors: Howard SP,Buckley JT

    更新日期:1985-03-01 00:00:00

  • Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis.

    abstract::We determined the nucleotide sequence of a 1.9-kilobase fragment of Pseudomonas paucimobilis SYK6 chromosomal DNA that included genes encoding protocatechuate 4,5-dioxygenase, the enzyme responsible for the aromatic ring fission of protocatechuate. Two open reading frames of 417 and 906 base pairs were found that had ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2704-2709.1990

    authors: Noda Y,Nishikawa S,Shiozuka K,Kadokura H,Nakajima H,Yoda K,Katayama Y,Morohoshi N,Haraguchi T,Yamasaki M

    更新日期:1990-05-01 00:00:00

  • Transcriptional activation of the Listeria monocytogenes hemolysin gene in Bacillus subtilis.

    abstract::The prfA gene of Listeria monocytogenes was recently reported to be required for expression of hly, which encodes a pore-forming hemolysin essential for pathogenicity (M. Leimeister-Wachter, C. Haffner, E. Domann, W. Goebel, and T. Chakraborty, Proc. Natl. Acad. Sci. USA 87:8336-8340, 1990). We demonstrate here that a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.4.1293-1298.1992

    authors: Freitag NE,Youngman P,Portnoy DA

    更新日期:1992-02-01 00:00:00

  • Genome-based analysis and gene dosage studies provide new insight into 3-hydroxy-4-methylvalerate biosynthesis in Ralstonia eutropha.

    abstract::Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02474-14

    authors: Saika A,Ushimaru K,Mizuno S,Tsuge T

    更新日期:2015-04-01 00:00:00

  • Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon.

    abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.245-254.1991

    authors: Eddy CK,Keshav KF,An H,Utt EA,Mejia JP,Ingram LO

    更新日期:1991-01-01 00:00:00

  • Tumor induction by Agrobacterium tumefaciens: specific transfer of bacterial deoxyribonucleic acid to plant tissue.

    abstract::When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.973-979.1971

    authors: Yajko DM,Hegeman GD

    更新日期:1971-12-01 00:00:00

  • Regulation of breakdown and synthesis of L-glutamate decarboxylase in Clostridium perfringens.

    abstract::L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1115-1123.1975

    authors: Cozzani I,Barsacchi R,Dibenedetto G,Saracchi L,Falcone G

    更新日期:1975-09-01 00:00:00

  • Bacillus subtilis pellicle formation proceeds through genetically defined morphological changes.

    abstract::Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00157-07

    authors: Kobayashi K

    更新日期:2007-07-01 00:00:00