Reproducible and rapid methods for the isolation and assay of a-factor, a yeast mating hormone.

abstract：:Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved...

journal_title：Journal of bacteriology

authors： De La Fuente L,Burr TJ,Hoch HC

更新日期：2007-10-01 00:00:00

abstract：:Chorismate mutase from Streptomyces aureofaciens was purified 12-fold. This enzyme preparation did not show any activity when tested for anthranilate synthetase, prephenate dehydrogenase, or prephenate dehydratase. The catalytic activity of chorismate mutase has a broad optimum between pH 7 and 8. The initial velocity...

journal_title：Journal of bacteriology

authors： Görisch H,Lingens F

更新日期：1973-05-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form biofilms is an important requirement for chronic colonization of human tissues and for persistence in implanted medical...

journal_title：Journal of bacteriology

authors： Vallet I,Diggle SP,Stacey RE,Cámara M,Ventre I,Lory S,Lazdunski A,Williams P,Filloux A

更新日期：2004-05-01 00:00:00

abstract：:Replication initiator proteins in bacteria not only allow DNA replication but also often regulate the rate of replication initiation as well. The regulation is mediated by limiting the synthesis or availability of initiator proteins. The applicability of this principle is demonstrated here for RctB, the replication in...

journal_title：Journal of bacteriology

authors： Pal D,Venkova-Canova T,Srivastava P,Chattoraj DK

更新日期：2005-11-01 00:00:00

abstract：UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...

journal_title：Journal of bacteriology

authors： Kitanovic S,Ames P,Parkinson JS

更新日期：2015-08-01 00:00:00

abstract：:A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...

journal_title：Journal of bacteriology

authors： Oosawa K,Mutoh N,Simon MI

更新日期：1988-06-01 00:00:00

abstract：:We isolated an unusual mutant in which the arabinose operon carried an araC lesion that led to a dominant fucose resistance while retaining recessive constitutivity. All previously characterized C(c) mutations have been recessive for both fucose resistance and constitutivity. ...

journal_title：Journal of bacteriology

authors： Nathanson NM,Schleif R

更新日期：1973-08-01 00:00:00

abstract：:SwmB is required for swimming motility in Synechococcus sp. strain WH8102. This highly repetitive 1.12-MDa polypeptide is associated with the cell surface, where it is arranged in a punctate manner. Inactivation of swmB does not affect the localization of SwmA, an S-layer protein also required for swimming. ...

journal_title：Journal of bacteriology

authors： McCarren J,Brahamsha B

更新日期：2007-02-01 00:00:00

abstract：:The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multip...

journal_title：Journal of bacteriology

authors： Lynd L,Kerby R,Zeikus JG

更新日期：1982-01-01 00:00:00

abstract：:After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by int...

journal_title：Journal of bacteriology

authors： Downard JS

更新日期：1988-10-01 00:00:00

abstract：:Billen, Daniel (University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Tex.), and Roger Hewitt. Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J. Bacteriol. 92:609-617. 1966.-A study has been made of the subsequent replicative ...

journal_title：Journal of bacteriology

authors： Billen D,Hewitt R

更新日期：1966-09-01 00:00:00

abstract：:As adhesion fimbriae are a major virulence factor for many pathogenic Gram-negative bacteria, they are also potential targets for antibodies. Fimbriae are commonly required for initiating the colonization that leads to disease, and their success as adhesion organelles lies in their ability to both initiate and sustain...

journal_title：Journal of bacteriology

authors： Singh B,Mortezaei N,Savarino SJ,Uhlin BE,Bullitt E,Andersson M

更新日期：2016-12-13 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:When incubated in the light under 100% oxygen, wild-type blue-green algae (Anacystis nidulans, Synechococcus cedrorum) die out rapidly at temperatures of 4 to 15 C, and at 35 C (or at 26 C in the case of S. cedrorum) in the absence of CO(2). Photosynthesis is impaired in these cells long before they die. Blocking of p...

journal_title：Journal of bacteriology

authors： Abeliovich A,Shilo M

更新日期：1972-09-01 00:00:00

abstract：:Members of Thermococcales harbor a number of genes encoding putative aminotransferase Class III enzymes. Here, we characterized the TK1211 protein from the hyperthermophilic archaeon Thermococcus kodakarensis The TK1211 gene was expressed in T. kodakarensis under the control of the strong, constitutive promoter of the...

journal_title：Journal of bacteriology

authors： Zheng RC,Lu XF,Tomita H,Hachisuka SI,Zheng YG,Atomi H

更新日期：2021-01-19 00:00:00

abstract：:Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

journal_title：Journal of bacteriology

authors： Ma Z,Masuda N,Foster JW

更新日期：2004-11-01 00:00:00

abstract：:A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1973-05-01 00:00:00

abstract：:Escherichia coli K-12 strains contain a cytoplasmic activity, peptidase N, capable of hydrolyzing alanine-p-nitroanilide. Mutations in the structural gene for the enzyme, pepN, were mapped, and the properties of mutant strains were examined. The pepN locus lay between ompF and asnS at approximately 20.8 min on the E. ...

journal_title：Journal of bacteriology

authors： McCaman MT,McPartland A,Villarejo MR

更新日期：1982-11-01 00:00:00

abstract：:The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made an...

journal_title：Journal of bacteriology

authors： Scheffers DJ,Robichon C,Haan GJ,den Blaauwen T,Koningstein G,van Bloois E,Beckwith J,Luirink J

更新日期：2007-10-01 00:00:00

abstract：:An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4...

journal_title：Journal of bacteriology

authors： Siegel EC

更新日期：1973-01-01 00:00:00

abstract：:PhoB is the response regulator of the Pho regulon. It is composed of two distinct domains, an N-terminal receiver domain and a C-terminal output domain that binds DNA and interacts with sigma(70) to activate transcription of the Pho regulon. Phosphorylation of the receiver domain is required for activation of the prot...

journal_title：Journal of bacteriology

authors： Ellison DW,McCleary WR

更新日期：2000-12-01 00:00:00

abstract：:A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Escherichia coli fabD mutant encoding a thermosensitive malonyl coenzyme A-acyl carrier protein transacylase. The B. subtilis genomic segment contains genes that encode three fatty acid synthetic proteins, malonyl coenzyme ...

journal_title：Journal of bacteriology

authors： Morbidoni HR,de Mendoza D,Cronan JE Jr

更新日期：1996-08-01 00:00:00

abstract：:Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...

journal_title：Journal of bacteriology

authors： Cybulska J,Jeljaszewicz J

更新日期：1966-03-01 00:00:00

abstract：:A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription f...

journal_title：Journal of bacteriology

authors： Murray EJ,Strauch MA,Stanley-Wall NR

更新日期：2009-11-01 00:00:00

abstract：:The characteristics of a system for the in vitro synthesis of mitochondrial deoxyribonucleic acid (mtDNA) in mitochondria isolated from Saccharomyces cerevisiae are described. In this system the exclusive product of the reaction is mtDNA. Under optimal conditions the initial rate of synthesis is close to the calculate...

journal_title：Journal of bacteriology

authors： Mattick JS,Hall RM

更新日期：1977-06-01 00:00:00

abstract：:The dctA gene, coding for the dicarboxylate transport protein, has an inducible promoter dependent on activation by the two-component sensor-regulator pair DctB and DctD. LacZ fusion analysis indicates that there is a single promoter for dctB and dctD. The dctA promoter is also induced by nitrogen limitation, an effec...

journal_title：Journal of bacteriology

authors： Reid CJ,Poole PS

更新日期：1998-05-01 00:00:00

abstract：:Numerous bacteria and mammalian cells harbor two enzymes, phosphopentomutase (PPM) and 2-deoxyribose 5-phosphate aldolase (DERA), involved in the interconversion between nucleosides and central carbon metabolism. In this study, we have examined the presence of this metabolic link in the hyperthermophilic archaeon, The...

journal_title：Journal of bacteriology

authors： Rashid N,Imanaka H,Fukui T,Atomi H,Imanaka T

更新日期：2004-07-01 00:00:00

abstract：:Corynebacterium diphtheriae, a Gram-positive, aerobic bacterium, is the causative agent of diphtheria and cutaneous infections. While mechanisms required for heme iron acquisition are well known in C. diphtheriae, systems involved in the acquisition of other metals such as zinc and manganese remain poorly characterize...

journal_title：Journal of bacteriology

authors： Peng ED,Oram DM,Battistel MD,Lyman LR,Freedberg DI,Schmitt MP

更新日期：2018-04-24 00:00:00

abstract：:A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

journal_title：Journal of bacteriology

authors： Hall BG

更新日期：1999-02-01 00:00:00

abstract：:Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...

journal_title：Journal of bacteriology

authors： Cho K,Fuqua C,Martin BS,Winans SC

更新日期：1996-04-01 00:00:00